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MicroRNA‑665 regulates the proliferation, apoptosis and adhesion of gastric cancer cells by binding to cadherin 3

  • Authors:
    • Xinhui Fang
    • Yangqiu Bai
    • Lida Zhang
    • Songze Ding
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology and Hepatology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, Henan 450003, P.R. China
    Copyright: © Fang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 494
    |
    Published online on: April 26, 2021
       https://doi.org/10.3892/ol.2021.12755
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Abstract

Numerous studies have reported that abnormal cadherin 3 (CDH3) and microRNA (miRNA/miR)‑665 expression can induce the progression of gastric cancer (GC). However, the mechanism of interaction between miR‑665 and CDH3 in GC requires further investigation. The present study aimed to investigate the influence of miR‑665 and CDH3 in GC development. The effect of miR‑665 and CDH3 on GC tissues and cell lines was examined using reverse transcription‑quantitative PCR. Subsequently, CDH3 protein expression in GC cell lines was detected using western blotting. To confirm the association between miR‑665 and CDH3, a dual‑luciferase reporter assay system was employed. Cell proliferation and adhesion were analyzed using BrdU ELISA, MTT and cell adhesion assays. Finally, caspase‑3 activity assay kit and FITC apoptosis detection kit were used for the determination of apoptosis of GC cells. The current findings confirmed the upregulation of CDH3 expression in GC cell lines and tissues. Experimental results indicated that CDH3 overexpression increased cell proliferation and adhesion, but decreased the apoptosis level of the cells. Similarly, the miR‑665 inhibitor enhanced cell proliferation and adhesion, but inhibited apoptosis of GC cells. Additionally, it was observed that CDH3 was a direct target of miR‑665 in GC cells and that miR‑665 inhibited CDH3 expression, thereby repressing the progression of GC. In conclusion, the present study suggested that by targeting CDH3, miR‑665 suppressed the progression of GC. These findings may provide a significant theoretical basis for future GC clinical therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Fang X, Bai Y, Zhang L and Ding S: MicroRNA‑665 regulates the proliferation, apoptosis and adhesion of gastric cancer cells by binding to cadherin 3. Oncol Lett 21: 494, 2021.
APA
Fang, X., Bai, Y., Zhang, L., & Ding, S. (2021). MicroRNA‑665 regulates the proliferation, apoptosis and adhesion of gastric cancer cells by binding to cadherin 3. Oncology Letters, 21, 494. https://doi.org/10.3892/ol.2021.12755
MLA
Fang, X., Bai, Y., Zhang, L., Ding, S."MicroRNA‑665 regulates the proliferation, apoptosis and adhesion of gastric cancer cells by binding to cadherin 3". Oncology Letters 21.6 (2021): 494.
Chicago
Fang, X., Bai, Y., Zhang, L., Ding, S."MicroRNA‑665 regulates the proliferation, apoptosis and adhesion of gastric cancer cells by binding to cadherin 3". Oncology Letters 21, no. 6 (2021): 494. https://doi.org/10.3892/ol.2021.12755
Copy and paste a formatted citation
x
Spandidos Publications style
Fang X, Bai Y, Zhang L and Ding S: MicroRNA‑665 regulates the proliferation, apoptosis and adhesion of gastric cancer cells by binding to cadherin 3. Oncol Lett 21: 494, 2021.
APA
Fang, X., Bai, Y., Zhang, L., & Ding, S. (2021). MicroRNA‑665 regulates the proliferation, apoptosis and adhesion of gastric cancer cells by binding to cadherin 3. Oncology Letters, 21, 494. https://doi.org/10.3892/ol.2021.12755
MLA
Fang, X., Bai, Y., Zhang, L., Ding, S."MicroRNA‑665 regulates the proliferation, apoptosis and adhesion of gastric cancer cells by binding to cadherin 3". Oncology Letters 21.6 (2021): 494.
Chicago
Fang, X., Bai, Y., Zhang, L., Ding, S."MicroRNA‑665 regulates the proliferation, apoptosis and adhesion of gastric cancer cells by binding to cadherin 3". Oncology Letters 21, no. 6 (2021): 494. https://doi.org/10.3892/ol.2021.12755
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