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Antimicrobial peptide 17BIPHE2 inhibits the proliferation of lung cancer cells in vitro and in vivo by regulating the ERK signaling pathway

  • Authors:
    • Tingting Yang
    • Jun Li
    • Qinqin Jia
    • Shisheng Zhan
    • Qiannan Zhang
    • Yarong Wang
    • Xiuqing Wang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Yinchuan Maternal and Child Health Care Hospital, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China, Department of Clinical Laboratory, Tangshan Gongren Hospital, Tangshan, Hebei 063000, P.R. China, Department of Laboratory Medicine, Health Center, Chun Rong, Gansu 745211, P.R. China, Department of Clinical Laboratory, Hebei Yanda Lu Daopei Hospital, Langfang, Hebei 065200, P.R. China, Department of Laboratory Medicine, College of Clinical Medicine, Shuangyi Campus, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region 750004, P.R. China, Department of Pathology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences, Tianjin 300020, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 501
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    Published online on: April 28, 2021
       https://doi.org/10.3892/ol.2021.12762
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Abstract

In 2018, there were 18.1 million new cancer cases and 9.6 million cancer‑related deaths worldwide, among which the incidence rate of lung cancer (11.6%) and fatality rate (18.4%) both ranked first. The antimicrobial peptide LL‑37 is an important component of the natural immune system and possesses several biological properties, including antibacterial, antiviral and anticancer effects. The antimicrobial peptide 17BIPHE2, the shortest synthetic peptide derivative of LL‑37, exhibits biological activities similar to those of LL‑37. The objective of the present study was to investigate the mechanism of action of exogenous 17BIPHE2 against lung cancer cells. The human lung adenocarcinoma cell line A549 was treated with 17BIPHE2. Changes in cell proliferation, migration, invasion, mitochondrial membrane potential (ΔΨm), and the levels of reactive oxygen species (ROS), Ca2+ and apoptosis‑related proteins, including BAX, BCL‑2 and ERK, were detected using flow cytometry, transmission electron microscopy and western blotting. The results showed that 17BIPHE2 significantly increased the apoptosis rate of A549 cells and elevated BAX expression, ERK phosphorylation, and ROS and Ca2+ levels, but decreased the expression of BCL‑2, ERK and Ki67. In addition, the peptide reduced ΔΨm and the cell migration ability of A549 cells and inhibited tumor growth. ERK inhibition significantly attenuated the anticancer effect of 17BIPHE2. The present observations suggested that 17BIPHE2 can effectively inhibit cancer cells by regulating the ERK signaling pathway.
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Spandidos Publications style
Yang T, Li J, Jia Q, Zhan S, Zhang Q, Wang Y and Wang X: Antimicrobial peptide 17BIPHE2 inhibits the proliferation of lung cancer cells <em>in vitro</em> and <em>in vivo</em> by regulating the ERK signaling pathway. Oncol Lett 22: 501, 2021.
APA
Yang, T., Li, J., Jia, Q., Zhan, S., Zhang, Q., Wang, Y., & Wang, X. (2021). Antimicrobial peptide 17BIPHE2 inhibits the proliferation of lung cancer cells <em>in vitro</em> and <em>in vivo</em> by regulating the ERK signaling pathway. Oncology Letters, 22, 501. https://doi.org/10.3892/ol.2021.12762
MLA
Yang, T., Li, J., Jia, Q., Zhan, S., Zhang, Q., Wang, Y., Wang, X."Antimicrobial peptide 17BIPHE2 inhibits the proliferation of lung cancer cells <em>in vitro</em> and <em>in vivo</em> by regulating the ERK signaling pathway". Oncology Letters 22.1 (2021): 501.
Chicago
Yang, T., Li, J., Jia, Q., Zhan, S., Zhang, Q., Wang, Y., Wang, X."Antimicrobial peptide 17BIPHE2 inhibits the proliferation of lung cancer cells <em>in vitro</em> and <em>in vivo</em> by regulating the ERK signaling pathway". Oncology Letters 22, no. 1 (2021): 501. https://doi.org/10.3892/ol.2021.12762
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Spandidos Publications style
Yang T, Li J, Jia Q, Zhan S, Zhang Q, Wang Y and Wang X: Antimicrobial peptide 17BIPHE2 inhibits the proliferation of lung cancer cells <em>in vitro</em> and <em>in vivo</em> by regulating the ERK signaling pathway. Oncol Lett 22: 501, 2021.
APA
Yang, T., Li, J., Jia, Q., Zhan, S., Zhang, Q., Wang, Y., & Wang, X. (2021). Antimicrobial peptide 17BIPHE2 inhibits the proliferation of lung cancer cells <em>in vitro</em> and <em>in vivo</em> by regulating the ERK signaling pathway. Oncology Letters, 22, 501. https://doi.org/10.3892/ol.2021.12762
MLA
Yang, T., Li, J., Jia, Q., Zhan, S., Zhang, Q., Wang, Y., Wang, X."Antimicrobial peptide 17BIPHE2 inhibits the proliferation of lung cancer cells <em>in vitro</em> and <em>in vivo</em> by regulating the ERK signaling pathway". Oncology Letters 22.1 (2021): 501.
Chicago
Yang, T., Li, J., Jia, Q., Zhan, S., Zhang, Q., Wang, Y., Wang, X."Antimicrobial peptide 17BIPHE2 inhibits the proliferation of lung cancer cells <em>in vitro</em> and <em>in vivo</em> by regulating the ERK signaling pathway". Oncology Letters 22, no. 1 (2021): 501. https://doi.org/10.3892/ol.2021.12762
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