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PLEK2 promotes osteosarcoma tumorigenesis and metastasis by activating the PI3K/AKT signaling pathway

  • Authors:
    • Yang Liu
    • Siting Yang
    • Feng Wang
    • Zheng Zhou
    • Wenjing Xu
    • Jingjing Xie
    • Linhui Qiao
    • Yanglin Gu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, The Affiliated Wuxi No. 2 People's Hospital of Nanjing Medical University, Wuxi, Jiangsu 214002, P.R. China, Department of Anesthesiology and Nursing, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Analysis Center, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China, Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Ultrasound, Wuxi Xishan People's Hospital, Wuxi, Jiangsu 214000, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 534
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    Published online on: May 18, 2021
       https://doi.org/10.3892/ol.2021.12795
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Abstract

Increasing evidence suggest that pleckstrin‑2 (PLEK2) acts as an oncogene in several malignancies. The present study aimed to investigate the effects of PLEK2 on osteosarcoma (OS) tumorigenesis and metastasis. PLEK2 expression in OS was analyzed via bioinformatics, reverse transcription‑quantitative PCR, western blot and immunohistochemistry analyses. The Cell Counting Kit‑8 (CCK‑8), colony formation and EdU assays were performed to assess the role of PLEK2 in OS cell proliferation. The pro‑metastatic effects of PLEK2 were assessed via the Transwell and wound healing assays. In addition, the PLEK2 downstream pathway was analyzed via bioinformatics analysis and verified via western blot analysis. The results demonstrated that PLEK2 expression was upregulated in both OS cell lines and specimens. The results of the CCK‑8, colony formation and EdU assays demonstrated that PLEK2 promoted OS cell proliferation in vitro. The in vivo experiments further demonstrated that PLEK2 knockdown significantly suppressed OS growth. In addition, the Transwell and wound healing assays indicated that PLEK2 promoted OS invasiveness in vitro, which was induced by the activation of the epithelial‑to‑mesenchymal transition process. Bioinformatics analysis revealed that PLEK2 can activate the phosphoinositide 3‑kinase (PI3K)/protein kinase B (AKT)/mechanistic target of rapamycin (mTOR) pathway, which was verified via western blot analysis. Taken together, the results of the present study suggest that PLEK2 may play a tumor‑promoting role in OS via the PI3K/AKT signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Yang S, Wang F, Zhou Z, Xu W, Xie J, Qiao L and Gu Y: PLEK2 promotes osteosarcoma tumorigenesis and metastasis by activating the PI3K/AKT signaling pathway. Oncol Lett 22: 534, 2021.
APA
Liu, Y., Yang, S., Wang, F., Zhou, Z., Xu, W., Xie, J. ... Gu, Y. (2021). PLEK2 promotes osteosarcoma tumorigenesis and metastasis by activating the PI3K/AKT signaling pathway. Oncology Letters, 22, 534. https://doi.org/10.3892/ol.2021.12795
MLA
Liu, Y., Yang, S., Wang, F., Zhou, Z., Xu, W., Xie, J., Qiao, L., Gu, Y."PLEK2 promotes osteosarcoma tumorigenesis and metastasis by activating the PI3K/AKT signaling pathway". Oncology Letters 22.1 (2021): 534.
Chicago
Liu, Y., Yang, S., Wang, F., Zhou, Z., Xu, W., Xie, J., Qiao, L., Gu, Y."PLEK2 promotes osteosarcoma tumorigenesis and metastasis by activating the PI3K/AKT signaling pathway". Oncology Letters 22, no. 1 (2021): 534. https://doi.org/10.3892/ol.2021.12795
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Yang S, Wang F, Zhou Z, Xu W, Xie J, Qiao L and Gu Y: PLEK2 promotes osteosarcoma tumorigenesis and metastasis by activating the PI3K/AKT signaling pathway. Oncol Lett 22: 534, 2021.
APA
Liu, Y., Yang, S., Wang, F., Zhou, Z., Xu, W., Xie, J. ... Gu, Y. (2021). PLEK2 promotes osteosarcoma tumorigenesis and metastasis by activating the PI3K/AKT signaling pathway. Oncology Letters, 22, 534. https://doi.org/10.3892/ol.2021.12795
MLA
Liu, Y., Yang, S., Wang, F., Zhou, Z., Xu, W., Xie, J., Qiao, L., Gu, Y."PLEK2 promotes osteosarcoma tumorigenesis and metastasis by activating the PI3K/AKT signaling pathway". Oncology Letters 22.1 (2021): 534.
Chicago
Liu, Y., Yang, S., Wang, F., Zhou, Z., Xu, W., Xie, J., Qiao, L., Gu, Y."PLEK2 promotes osteosarcoma tumorigenesis and metastasis by activating the PI3K/AKT signaling pathway". Oncology Letters 22, no. 1 (2021): 534. https://doi.org/10.3892/ol.2021.12795
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