Open Access

Changes in SLIT2 expression are associated with the migration of human ovarian clear cell carcinoma cells

  • Authors:
    • Cuei-Jyuan Lin
    • Way-Ren Huang
    • Chia-Zhen Wu
    • Ruo-Chia Tseng
  • View Affiliations

  • Published online on: May 24, 2021     https://doi.org/10.3892/ol.2021.12812
  • Article Number: 551
  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Ovarian clear cell carcinoma (OCCC) is characterized by a poor survival of patients, which is mainly due to metastasis and treatment failure. Slit guidance ligand 2 (SLIT2), a secreted protein, has been reported to modulate the migration of neural cells and human cancer cells. However, the effect of changes in SLIT2 expression on the regulation of cell migration in OCCC remains unknown. The present study examined alterations in SLIT2 expression using OCCC cell models, including low‑ and high‑mobility SKOV3 cells, as well as OCCC tissues. DNA methylation analysis suggested that promoter hypermethylation was responsible for the low expression levels of SLIT2 in OCCC cells. The demethylating agent 5‑Aza‑deoxycytosine was able to restore SLIT2 expression at both the mRNA and protein levels in high‑mobility SKOV3 cells that harbored the relevant methylated promoter. Overexpression of SLIT2 inhibited the migration of high‑mobility OCCC cells, as well as decreased the protein expression levels of β‑catenin, phosphorylated (p)AKT and snail family transcriptional repressor 1 (SNAI1). On the other hand, knockdown of SLIT2 increased the migration of low‑mobility OCCC cells, and enhanced the protein expression levels of β‑catenin, pAKT and SNAI1. Overall, the results of the present study provided evidence that low expression levels of SLIT2 were associated with increased OCCC cell migration, and that SLIT2 may act as a suppressor gene of cancer cell migration.
View Figures
View References

Related Articles

Journal Cover

July-2021
Volume 22 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

The Cancer Story
Copy and paste a formatted citation
x
Spandidos Publications style
Lin C, Huang W, Wu C and Tseng R: Changes in SLIT2 expression are associated with the migration of human ovarian clear cell carcinoma cells. Oncol Lett 22: 551, 2021
APA
Lin, C., Huang, W., Wu, C., & Tseng, R. (2021). Changes in SLIT2 expression are associated with the migration of human ovarian clear cell carcinoma cells. Oncology Letters, 22, 551. https://doi.org/10.3892/ol.2021.12812
MLA
Lin, C., Huang, W., Wu, C., Tseng, R."Changes in SLIT2 expression are associated with the migration of human ovarian clear cell carcinoma cells". Oncology Letters 22.1 (2021): 551.
Chicago
Lin, C., Huang, W., Wu, C., Tseng, R."Changes in SLIT2 expression are associated with the migration of human ovarian clear cell carcinoma cells". Oncology Letters 22, no. 1 (2021): 551. https://doi.org/10.3892/ol.2021.12812