Open Access

Triptolide inhibits matrix metalloproteinase-9 expression and invasion of breast cancer cells through the inhibition of NF-κB and AP-1 signaling pathways

  • Authors:
    • On-Yu Hong
    • Hye-Yeon Jang
    • Kwang-Hyun Park
    • Young-Ju Jeong
    • Jong-Suk Kim
    • Hee Suk Chae
  • View Affiliations

  • Published online on: May 27, 2021     https://doi.org/10.3892/ol.2021.12823
  • Article Number: 562
  • Copyright: © Hong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Triptolide is a diterpenoid epoxide that is endogenously produced by the thunder god vine, Tripterygium wilfordii Hook F. Triptolide has demonstrated a variety of biological activities, including anticancer activities, in previous studies. Invasion and metastasis are the leading causes of mortality for patients with breast cancer, and the increased expression of matrix metalloproteinase-9 (MMP-9) has been shown to be associated with breast cancer invasion. Therefore, the aim of the present study was to investigate the effect of triptolide on 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced cell invasion and MMP-9 expression in breast cancer cells. The expression of signal molecules was examined by western blotting, zymography and quantitative polymerase chain reaction; an electrophoretic mobility gel shift assay was also used, and cell invasiveness was measured by an in vitro Matrigel invasion assay. The MCF-7 human breast cancer cell line was treated with triptolide at the highest concentrations at which no marked cytotoxicity was evident. The results demonstrated that triptolide decreased the expression of MMP-9 through inhibition of the TPA-induced phosphorylation of extracellular signal-regulated kinase (ERK) and the downregulation of nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) activity. In addition, a Transwell assay revealed that triptolide reduced the ability of MCF-7 cells to invade Matrigel. These data demonstrate that the anti-invasive effect of triptolide is associated with the inhibition of ERK signaling and NF-κB and AP-1 activation, and suggest that triptolide may be a promising drug for breast cancer.
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July-2021
Volume 22 Issue 1

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Spandidos Publications style
Hong O, Jang H, Park K, Jeong Y, Kim J and Chae HS: Triptolide inhibits matrix metalloproteinase-9 expression and invasion of breast cancer cells through the inhibition of NF-κB and AP-1 signaling pathways. Oncol Lett 22: 562, 2021
APA
Hong, O., Jang, H., Park, K., Jeong, Y., Kim, J., & Chae, H.S. (2021). Triptolide inhibits matrix metalloproteinase-9 expression and invasion of breast cancer cells through the inhibition of NF-κB and AP-1 signaling pathways. Oncology Letters, 22, 562. https://doi.org/10.3892/ol.2021.12823
MLA
Hong, O., Jang, H., Park, K., Jeong, Y., Kim, J., Chae, H. S."Triptolide inhibits matrix metalloproteinase-9 expression and invasion of breast cancer cells through the inhibition of NF-κB and AP-1 signaling pathways". Oncology Letters 22.1 (2021): 562.
Chicago
Hong, O., Jang, H., Park, K., Jeong, Y., Kim, J., Chae, H. S."Triptolide inhibits matrix metalloproteinase-9 expression and invasion of breast cancer cells through the inhibition of NF-κB and AP-1 signaling pathways". Oncology Letters 22, no. 1 (2021): 562. https://doi.org/10.3892/ol.2021.12823