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si‑MALAT1 attenuates thymic cancer cell proliferation and promotes apoptosis via the miR‑145‑5p/HMGA2 pathway

  • Authors:
    • Sheng Tan
    • Jili Chen
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Surgery, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, P.R. China, Department of Ophthalmology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, P.R. China
    Copyright: © Tan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 585
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    Published online on: June 3, 2021
       https://doi.org/10.3892/ol.2021.12846
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Abstract

Metastasis‑associated‑lung‑adenocarcinoma-transcript‑1 (MALAT1) is a long non‑coding RNA that is considered a potential tumor marker. The present study aimed to investigate the effect and mechanism of MALAT1 on cell proliferation and apoptosis in thymic cancer cells. IU‑TAB‑1, A549, HCT‑116 and 293T cells were screened by reverse transcription‑quantitative PCR to assess high‑mobility group AT‑hook 2 (HMGA2) expression in various types of cancer cells and were transfected with small interfering (si)RNA targeting MALAT1 (si‑MALAT1). Cell proliferation was evaluated by Cell Counting Kit‑8 assay. Cell apoptosis and cell cycle were examined using flow cytometry. The protein expression of cyclin D1, cyclin E, Bax, Bcl‑2 and HMGA2 was determined by western blot analysis, while the associations between MALAT1 and microRNA (miR)‑145‑5p and between HMGA2 and miR‑145‑5p were determined by luciferase reporter assay. Among the four cell lines evaluated, IU‑TAB‑1 showed the highest expression of MALAT1; thus, IU‑TAB‑1 cells were selected for subsequent experiments. Compared with the findings in the control group, si‑MALAT1 significantly decreased the cell proliferation of IU‑TAB‑1 cells, whereas the apoptosis levels and number of cells in G2 phase were increased. The protein expression levels of cyclin D1, cyclin E, Bcl‑2 and HMGA2 were significantly decreased in the si‑MALAT1 group compared with those in the control group, while Bax levels were significantly increased. After treatment with si‑MALAT1 in combination with miR‑145‑5p mimics or inhibitors, cell proliferation and apoptosis were respectively enhanced and inhibited in IU‑TAB‑1 cells. miR‑145‑5p inhibited the luciferase activity of IU‑TAB‑1 cells transfected with the MALAT1 or HMGA2 3' untranslated region. In conclusion, si‑MALAT1 significantly attenuated cell proliferation and apoptosis via the miR‑145‑5p/HMGA2 pathway in thymic cancer cells.
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1 

Fink C, Henzler T, Shirinova A, Apfaltrer P and Wasser K: Thoracic magnetic resonance imaging: Pulmonary thromboembolism. J Thorac Imaging. 28:171–177. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Qureshi S, West M and Kirk AJB: Surgical resection for thymic malignancy. Lung Cancer. 63 (Suppl):S382009. View Article : Google Scholar

3 

Gutschner T, Monika H and Diederichs S: MALAT1-a paradigm for long noncoding RNA function in cancer. J Mol Med (Berl). 91:791–801. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Gutschner T, Hämmerle M, Eissmann M, Hsu J, Kim Y, Hung G, Revenko A, Arun G, Stentrup M, Gross M, et al: The Noncoding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells. Cancer Res. 73:1180–1189. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Hirata H, Hinoda Y, Shahryari V, Deng G, Nakajima K, Tabatabai ZL, Ishii N and Dahiya R: Long noncoding RNA MALAT1 promotes aggressive renal cell carcinoma through Ezh2 and interacts with miR-205. Cancer Res. 75:1322–1331. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Hu L, Wu Y, Tan D, Meng H, Wang K, Bai Y and Yang K: Up-regulation of long noncoding RNA MALAT1 contributes to proliferation and metastasis in esophageal squamous cell carcinoma. J Exp Clin Cancer Res. 34:72015. View Article : Google Scholar : PubMed/NCBI

7 

Zhang Y, Wang T, Huang HQ, Li W, Cheng XL and Yang J: Human MALAT-1 long non-coding RNA is overexpressed in cervical cancer metastasis and promotes cell proliferation, invasion and migration. J BUON. 20:1497–1503. 2016.

8 

Han Y, Wu Z, Wu T, Huang Y, Cheng Z, Li X, Sun T, Xie X, Zhou Y and Du Z: Tumor-suppressive function of long noncoding RNA MALAT1 in glioma cells by downregulation of MMP2 and inactivation of ERK/MAPK signaling. Cell Death Dis. 7:e21232016. View Article : Google Scholar : PubMed/NCBI

9 

Konishi H, Ichikawa D, Yamamoto Y, Arita T, Shoda K, Hiramoto H, Hamada J, Itoh H, Fujita Y, Komatsu S, et al: Plasma level of metastasis-associated lung adenocarcinoma transcript 1 is associated with liver damage and predicts development of hepatocellular carcinoma. Cancer Sci. 107:149–154. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Narita M, Narita M, Krizhanovsky V, Nuñez S, Chicas A, Hearn SA, Myers MP and Lowe SW: A novel role for high-mobility group a proteins in cellular senescence and heterochromatin formation. Cell. 126:503–514. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Di Cello F, Hillion J, Hristov A, Wood LJ, Mukherjee M, Schuldenfrei A, Kowalski J, Bhattacharya R, Ashfaq R and Resar LM: HMGA2 participates in transformation in human lung cancer. Mol Cancer Res. 6:743–750. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Malek A, Bakhidze E, Noske A, Sers C, Aigner A, Schäfer R and Tchernitsa O: HMGA2 gene is a promising target for ovarian cancer silencing therapy. Int J Cancer. 123:348–356. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Huang ML, Chen CC and Chang LC: Gene expressions of HMGI-C and HMGI(Y) are associated with stage and metastasis in colorectal cancer. Int J Colorectal Dis. 24:1281–1286. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Hristov AC, Cope L, Reyes MD, Singh M, Iacobuzio-Donahue C, Maitra A and Resar LM: HMGA2 protein expression correlates with lymph node metastasis and increased tumor grade in pancreatic ductal adenocarcinoma. Mod Pathol. 22:43–49. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Bartuma H, Panagopoulos I, Collin A, Trombetta D, Domanski HA, Mandahl N and Mertens F: Expression levels of HMGA2 in adipocytic tumors correlate with morphologic and cytogenetic subgroups. Mol Cancer. 8:362009. View Article : Google Scholar : PubMed/NCBI

16 

Livak KJ and Schmittgen TDL: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Shi D and Zhang Y, Lu R and Zhang Y: The long non-coding RNA MALAT1 interacted with miR-218 modulates choriocarcinoma growth by targeting Fbxw8. Biomed Pharmacother. 97:543–550. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Bai RM and Wang CX: Progress of long non-coding RNA MALAT1 in tumor research. J Clin Oncol. 21:1139–1145. 2016.

19 

Liu S, Song L, Zeng S and Zhang L: MALAT1-miR-124-RBG2 axis is involved in growth and invasion of HR-HPV-positive cervical cancer cells. Tumour Biol. 37:633–640. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Wang SH, Zhang WJ, Wu XC, Zhang MD, Weng MZ, Zhou D, Wang JD and Quan ZW: Long non-coding RNA Malat1 promotes gallbladder cancer development by acting as a molecular sponge to regulate miR-206. Oncotarget. 7:37857–37867. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Yang F, Yi F, Han X, Du Q and Liang Z: MALAT-1 interacts with hnRNP C in cell cycle regulation. FEBS Lett. 587:3175–3181. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Miyagawa R, Tano K, Mizuno R, Nakamura Y, Ijiri K, Rakwal R, Shibato J, Masuo Y, Mayeda A, Hirose T and Akimitsu N: Identification of cis- and trans-acting factors involved in the localization of MALAT-1 noncoding RNA to nuclear speckles. RNA. 18:738–751. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Zhang YJ, Tang XM, Shi MM, Wen CL and Shen BY: MiR-216a decreases MALAT1 expression, induces G2/M arrest and apoptosis in pancreatic cancer cells. Biochem Biophys Res Commun. 483:816–822. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Yao K, Zhang GJ, Huang ZM and Yao GX: Expression and function of long non-coding RNA-MALAT1 in liver cancer. Chin J Gen Surg. 1:90–96. 2016.

25 

Kangboonruang K, Wongtrakoongate P, Lertsuwan K, Khachonkham S, Changkaew P, Tangboonduangjit P, Siripoon T, Ngamphaiboon N and Chairoungdua A: MALAT1 decreases the sensitivity of head and neck squamous cell carcinoma cells to radiation and cisplatin. Anticancer Res. 40:2645–2655. 2020. View Article : Google Scholar : PubMed/NCBI

26 

Esquela-Kerscher A and Slack FJ: Oncomirs-microRNAs with a role in cancer. Nat Rev Cancer. 6:259–269. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Li Y, Li Y, Liu J, Fan Y, Li X, Dong M, Liu H and Chen J: Expression levels of microRNA-145 and microRNA-10b are associated with metastasis in non-small cell lung cancer. Cancer Biol Ther. 17:272–279. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Ding Y, Zhang C, Zhang J, Zhang N, Li T, Fang J, Zhang Y, Zuo F, Tao Z, Tang S, et al: miR-145 inhibits proliferation and migration of breast cancer cells by directly or indirectly regulating TGF-β1 expression. Int J Oncol. 50:1701–1710. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Zhou X, Yue Y, Wang R, Gong B and Duan Z: MicroRNA-145 inhibits tumorigenesis and invasion of cervical cancer stem cells. Int J Oncol. 50:853–862. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Xie SG, Xie YY, Zhang LL and Huang Q: Effect of miR-145 targeting DAB2 on the migration and invasion of prostate cancer PC3 cells. Heredity. 36:50–57. 2014.PubMed/NCBI

31 

Sun R, Qin C, Jiang B, Fang S, Pan X, Peng L, Liu Z, Li W, Li Y and Li G: Down-regulation of MALAT1 inhibits cervical cancer cell invasion and metastasis by inhibition of epithelial-mesenchymal transition. Mol Biosyst. 12:952–962. 2016. View Article : Google Scholar : PubMed/NCBI

32 

Xu S, Sui S, Zhang J, Bai N, Shi Q, Zhang G, Gao S, You Z, Zhan C, Liu F and Pang D: Downregulation of long noncoding RNA MALAT1 induces epithelial-to-mesenchymal transition via the PI3K-AKT pathway in breast cancer. Int J Clin Exp Pathol. 8:4881–4891. 2015.PubMed/NCBI

33 

Rong F, Liu L, Zou C, Zeng J and Xu Y: MALAT1 promotes cell tumorigenicity through regulating miR-515-5p/EEF2 axis in non-small cell lung cancer. Cancer Manag Res. 12:7691–7701. 2020. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Tan S and Chen J: si‑MALAT1 attenuates thymic cancer cell proliferation and promotes apoptosis via the miR‑145‑5p/HMGA2 pathway. Oncol Lett 22: 585, 2021.
APA
Tan, S., & Chen, J. (2021). si‑MALAT1 attenuates thymic cancer cell proliferation and promotes apoptosis via the miR‑145‑5p/HMGA2 pathway. Oncology Letters, 22, 585. https://doi.org/10.3892/ol.2021.12846
MLA
Tan, S., Chen, J."si‑MALAT1 attenuates thymic cancer cell proliferation and promotes apoptosis via the miR‑145‑5p/HMGA2 pathway". Oncology Letters 22.2 (2021): 585.
Chicago
Tan, S., Chen, J."si‑MALAT1 attenuates thymic cancer cell proliferation and promotes apoptosis via the miR‑145‑5p/HMGA2 pathway". Oncology Letters 22, no. 2 (2021): 585. https://doi.org/10.3892/ol.2021.12846
Copy and paste a formatted citation
x
Spandidos Publications style
Tan S and Chen J: si‑MALAT1 attenuates thymic cancer cell proliferation and promotes apoptosis via the miR‑145‑5p/HMGA2 pathway. Oncol Lett 22: 585, 2021.
APA
Tan, S., & Chen, J. (2021). si‑MALAT1 attenuates thymic cancer cell proliferation and promotes apoptosis via the miR‑145‑5p/HMGA2 pathway. Oncology Letters, 22, 585. https://doi.org/10.3892/ol.2021.12846
MLA
Tan, S., Chen, J."si‑MALAT1 attenuates thymic cancer cell proliferation and promotes apoptosis via the miR‑145‑5p/HMGA2 pathway". Oncology Letters 22.2 (2021): 585.
Chicago
Tan, S., Chen, J."si‑MALAT1 attenuates thymic cancer cell proliferation and promotes apoptosis via the miR‑145‑5p/HMGA2 pathway". Oncology Letters 22, no. 2 (2021): 585. https://doi.org/10.3892/ol.2021.12846
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