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Pseudogene legumain promotes thyroid carcinoma progression via the microRNA‑495/autophagy pathway

  • Authors:
    • Jie Sun
    • Yicheng Peng
    • Jianxia Liu
    • Hao Zhou
    • Liang Sun
    • Qin He
    • Enqiao Yu
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 616
    |
    Published online on: June 24, 2021
       https://doi.org/10.3892/ol.2021.12877
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Abstract

The pseudogene legumain (LGMN) has been reported to regulate cancer cell biology. However, the role of LGMN in thyroid carcinoma remains unknown. Herein, Cell Counting Kit 8 and Transwell assays were performed to evaluate cellular proliferation and invasion capacity, respectively. In addition, a tube formation assay was performed to assess HUVEC angiogenesis. The results showed that LGMN depletion attenuated cellular proliferation, invasion and tube formation ability, and that LGMN expression was dysregulated in thyroid carcinoma tumors. Furthermore, patients with high LGMN expression levels exhibited a lower overall survival rate than those with low expression levels. LGMN and microRNA (miR)‑495 modulated the expression levels of autophagy‑related gene 3 (ATG3) and p62. Finally, ATG3 overexpression rescued the LGMN‑regulated thyroid carcinoma phenotype. In conclusion, LGMN was found to promote thyroid carcinoma progression via the miR‑495/autophagy axis, thus providing novel insights for understanding the pathogenesis of thyroid carcinoma.
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1 

Borran S, Ahmadi G, Rezaei S, Anari MM, Modabberi M, Azarash Z, Razaviyan J, Derakhshan M, Akhbari M and Mirzaei H: Circular RNAs: New players in thyroid cancer. Pathol Res Pract. 216:1532172020. View Article : Google Scholar : PubMed/NCBI

2 

Rossi ED, Faquin WC and Pantanowitz L: Cytologic features of aggressive variants of follicular-derived thyroid carcinoma. Cancer Cytopathol. 127:432–446. 2019. View Article : Google Scholar : PubMed/NCBI

3 

Tang KT and Lee CH: BRAF mutation in papillary thyroid carcinoma: Pathogenic role and clinical implications. J Chin Med Assoc. 73:113–128. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Ito Y, Jikuzono T, Higashiyama T, Asahi S, Tomoda C, Takamura Y, Miya A, Kobayashi K, Matsuzuka F, Kuma K and Miyauchi A: Clinical significance of lymph node metastasis of thyroid papillary carcinoma located in one lobe. World J Surg. 30:1821–1828. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Zhao J, Xu C, Yao J, Yu C, Liao L and Dong J: Statins and thyroid carcinoma: A meta-analysis. Cell Physiol Biochem. 47:1422–1431. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Stewart GL, Enfield KSS, Sage AP, Martinez VD, Minatel BC, Pewarchuk ME, Marshall EA and Lam WL: Aberrant expression of pseudogene-derived lncRNAs as an alternative mechanism of cancer gene regulation in lung adenocarcinoma. Front Genet. 10:1382019. View Article : Google Scholar : PubMed/NCBI

7 

Shih JH, Chen HY, Lin SC, Yeh YC, Shen R, Lang YD, Wu DC, Chen CY, Chen RH, Chou TY and Jou YS: Integrative analyses of noncoding RNAs reveal the potential mechanisms augmenting tumor malignancy in lung adenocarcinoma. Nucleic Acids Res. 48:1175–1191. 2020. View Article : Google Scholar : PubMed/NCBI

8 

Poliseno L: Pseudogenes: Newly discovered players in human cancer. Sci Signal. 5:re52012. View Article : Google Scholar : PubMed/NCBI

9 

Xiao-Jie L, Ai-Mei G, Li-Juan J and Jiang X: Pseudogene in cancer: Real functions and promising signature. J Med Genet. 52:17–24. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Lin JD, Fu SS, Chen JY, Lee CH, Chau WK, Cheng CW, Wang YH, Lin YF, Fang WF and Tang KT: Clinical manifestations and gene expression in patients with conventional papillary thyroid carcinoma carrying the BRAF(V600E) mutation and BRAF pseudogene. Thyroid. 26:691–704. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Huang JL, Cao SW, Ou QS, Yang B, Zheng SH, Tang J, Chen J, Hu YW, Zheng L and Wang Q: The long non-coding RNA PTTG3P promotes cell growth and metastasis via up-regulating PTTG1 and activating PI3K/AKT signaling in hepatocellular carcinoma. Mol Cancer. 17:932018. View Article : Google Scholar : PubMed/NCBI

12 

Kong Y, Zhang L, Huang Y, He T, Zhang L, Zhao X, Zhou X, Zhou D, Yan Y, Zhou J, et al: Pseudogene PDIA3P1 promotes cell proliferation, migration and invasion, and suppresses apoptosis in hepatocellular carcinoma by regulating the p53 pathway. Cancer Lett. 407:76–83. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Sun M, Nie FQ, Zang C, Wang Y, Hou J, Wei C, Li W, He X and Lu KH: The pseudogene DUXAP8 promotes non-small-cell lung cancer cell proliferation and invasion by epigenetically silencing EGR1 and RHOB. Mol Ther. 25:739–751. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Liao K, Qian Z, Zhang S, Chen B, Li Z, Huang R, Cheng L, Wang T, Yang R, Lan J, et al: The LGMN pseudogene promotes tumor progression by acting as a miR-495-3p sponge in glioblastoma. Cancer Lett. 490:111–123. 2020. View Article : Google Scholar : PubMed/NCBI

15 

Kim YW, Kim EY, Jeon D, Liu JL, Kim HS, Choi JW and Ahn WS: Differential microRNA expression signatures and cell type-specific association with Taxol resistance in ovarian cancer cells. Drug Des Devel Ther. 8:293–314. 2014.PubMed/NCBI

16 

Duan W, Kong X, Li J, Li P, Zhao Y, Liu T, Binang HB, Wang Y, Du L and Wang C: LncRNA AC010789.1 promotes colorectal cancer progression by targeting microRNA-432-3p/ZEB1 axis and the Wnt/β-catenin signaling pathway. Front Cell Dev Biol. 8:5653552020. View Article : Google Scholar : PubMed/NCBI

17 

Kafshdooz L, Pourfathi H, Akbarzadeh A, Kafshdooz T, Razban Z, Sheervalilou R, Ebrahimi Sadr N, Khalilov R, Saghfi S, Kavetskyy T, et al: The role of microRNAs and nanoparticles in ovarian cancer: A review. Artif Cells Nanomed Biotechnol. 46:241–247. 2018. View Article : Google Scholar : PubMed/NCBI

18 

Han D, Li J, Wang H, Su X, Hou J, Gu Y, Qian C, Lin Y, Liu X, Huang M, et al: Circular RNA circMTO1 acts as the sponge of microRNA-9 to suppress hepatocellular carcinoma progression. Hepatology. 66:1151–1164. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Ye M, Dong S, Hou H, Zhang T and Shen M: Oncogenic role of long noncoding RNAMALAT1 in thyroid cancer progression through regulation of the miR-204/IGF2BP2/m6A-MYC signaling. Mol Ther Nucleic Acids. 23:1–12. 2021. View Article : Google Scholar : PubMed/NCBI

20 

Shu T, Yang L, Sun L, Lu J and Zhan X: CircHIPK3 promotes thyroid cancer tumorigenesis and invasion through the Mirna-338-3p/RAB23 axis. Med Princ Pract. Oct 26–2020.(Epub ahead of Print). View Article : Google Scholar

21 

Qian J, Garg A, Li F, Shen Q and Xiao K: LncRNA LUNAR1 accelerates colorectal cancer progression by targeting the miR4953p/MYCBP axis. Int J Oncol. 57:1157–1168. 2020.PubMed/NCBI

22 

Levy JMM, Towers CG and Thorburn A: Targeting autophagy in cancer. Nat Rev Cancer. 17:528–542. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Pagotto A, Pilotto G, Mazzoldi EL, Nicoletto MO, Frezzini S, Pastò A and Amadori A: Autophagy inhibition reduces chemoresistance and tumorigenic potential of human ovarian cancer stem cells. Cell Death Dis. 8:e29432017. View Article : Google Scholar : PubMed/NCBI

24 

Chude CI and Amaravadi RK: Targeting autophagy in cancer: Update on clinical trials and novel inhibitors. Int J Mol Sci. 18:12792017. View Article : Google Scholar : PubMed/NCBI

25 

Amaravadi R, Kimmelman AC and White E: Recent insights into the function of autophagy in cancer. Genes Dev. 30:1913–1930. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Wei W, Hardin H and Luo QY: Targeting autophagy in thyroid cancers. Endocr Relat Cancer. 26:R181–R194. 2019. View Article : Google Scholar : PubMed/NCBI

27 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Yan L, Yue C, Xu Y, Jiang X, Zhang L and Wu J: Identification of potential diagnostic and prognostic pseudogenes in hepatocellular carcinoma based on pseudogene-miRNA-mRNA competitive network. Med Sci Monit. 26:e9218952020. View Article : Google Scholar : PubMed/NCBI

29 

Hou Z, Wang Y, Xia N, Lv T, Yuan X and Song Y: Pseudogene KRT17P3 drives cisplatin resistance of human NSCLC cells by modulating miR-497-5p/mTOR. Cancer Sci. 112:275–286. 2021. View Article : Google Scholar : PubMed/NCBI

30 

Li W, Yang Y, Hou X, Zhuang H, Wu Z, Li Z, Guo R, Chen H, Lin C, Zhong W, et al: MicroRNA-495 regulates starvation-induced autophagy by targeting ATG3. FEBS Lett. 590:726–738. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Somuncu E, Karatas A, Ferahman S, Saygili N, Yilmaz E, Ozturk O and Kapan M: The investigation of foxe1 variations in papillary thyroid carcinoma. Int J Clin Exp Pathol. 8:13458–13464. 2015.PubMed/NCBI

32 

Li J, Jiang L, Liu Z, Li Y, Xu Y and Liu H: Oncogenic pseudogene DUXAP10 knockdown suppresses proliferation and invasion and induces apoptosis of papillary thyroid carcinoma cells by inhibition of Akt/mTOR pathway. Clin Exp Pharmacol Physiol. 47:1473–1483. 2020. View Article : Google Scholar : PubMed/NCBI

33 

Eun JW, Kim HS, Shen Q, Yang HD, Kim SY, Yoon JH, Park WS, Lee JY and Nam SW: MicroRNA-495-3p functions as a tumor suppressor by regulating multiple epigenetic modifiers in gastric carcinogenesis. J Pathol. 244:107–119. 2018. View Article : Google Scholar : PubMed/NCBI

34 

Chen X, Xu Y, Liao X, Liao R, Zhang L, Niu K, Li T, Li D, Chen Z, Duan Y and Sun J: Plasma miRNAs in predicting radiosensitivity in non-small cell lung cancer. Tumour Biol. 37:11927–11936. 2016. View Article : Google Scholar : PubMed/NCBI

35 

Halvorsen AR, Sandhu V, Sprauten M, Flote VG, Kure EH, Brustugun OT and Helland Å: Circulating microRNAs associated with prolonged overall survival in lung cancer patients treated with nivolumab. Acta Oncol. 57:1225–1231. 2018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun J, Peng Y, Liu J, Zhou H, Sun L, He Q and Yu E: Pseudogene legumain promotes thyroid carcinoma progression via the microRNA‑495/autophagy pathway. Oncol Lett 22: 616, 2021.
APA
Sun, J., Peng, Y., Liu, J., Zhou, H., Sun, L., He, Q., & Yu, E. (2021). Pseudogene legumain promotes thyroid carcinoma progression via the microRNA‑495/autophagy pathway. Oncology Letters, 22, 616. https://doi.org/10.3892/ol.2021.12877
MLA
Sun, J., Peng, Y., Liu, J., Zhou, H., Sun, L., He, Q., Yu, E."Pseudogene legumain promotes thyroid carcinoma progression via the microRNA‑495/autophagy pathway". Oncology Letters 22.2 (2021): 616.
Chicago
Sun, J., Peng, Y., Liu, J., Zhou, H., Sun, L., He, Q., Yu, E."Pseudogene legumain promotes thyroid carcinoma progression via the microRNA‑495/autophagy pathway". Oncology Letters 22, no. 2 (2021): 616. https://doi.org/10.3892/ol.2021.12877
Copy and paste a formatted citation
x
Spandidos Publications style
Sun J, Peng Y, Liu J, Zhou H, Sun L, He Q and Yu E: Pseudogene legumain promotes thyroid carcinoma progression via the microRNA‑495/autophagy pathway. Oncol Lett 22: 616, 2021.
APA
Sun, J., Peng, Y., Liu, J., Zhou, H., Sun, L., He, Q., & Yu, E. (2021). Pseudogene legumain promotes thyroid carcinoma progression via the microRNA‑495/autophagy pathway. Oncology Letters, 22, 616. https://doi.org/10.3892/ol.2021.12877
MLA
Sun, J., Peng, Y., Liu, J., Zhou, H., Sun, L., He, Q., Yu, E."Pseudogene legumain promotes thyroid carcinoma progression via the microRNA‑495/autophagy pathway". Oncology Letters 22.2 (2021): 616.
Chicago
Sun, J., Peng, Y., Liu, J., Zhou, H., Sun, L., He, Q., Yu, E."Pseudogene legumain promotes thyroid carcinoma progression via the microRNA‑495/autophagy pathway". Oncology Letters 22, no. 2 (2021): 616. https://doi.org/10.3892/ol.2021.12877
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