lnc‑NLC1‑C inhibits migration, invasion and autophagy of glioma cells by targeting miR‑383 and regulating PRDX‑3 expression

Xu,Zhou; Chen,Qianxue; Zeng,Xingruo; Li,Mingchang; Liao,Jianming

doi:10.3892/ol.2021.12901

lnc‑NLC1‑C inhibits migration, invasion and autophagy of glioma cells by targeting miR‑383 and regulating PRDX‑3 expression

Authors:
- Zhou Xu
- Qianxue Chen
- Xingruo Zeng
- Mingchang Li
- Jianming Liao
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Affiliations: Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China, Department of Nephrology and Rheumatology, The Central Hospital of Wuhan, Huazhong University of Science and Technology, Wuhan, Hubei 430014, P.R. China
Published online on: July 7, 2021 https://doi.org/10.3892/ol.2021.12901
Article Number: 640

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Abstract

Long non‑coding RNAs (lncRNAs) serve an important role in tumor progression, and their abnormal expression is associated with tumor development. The lncRNA narcolepsy candidate region 1 gene C (lnc‑NLC1‑C) is involved in numerous types of cancer, but its biological function in glioma remains unknown. In the present study, lnc‑NLC1‑C expression was detected using reverse transcription‑quantitative (RT‑q)PCR in U251, SHG44, U87MG and U118MG glioma cells. U87MG cells were transfected with lnc‑NLC1‑C overexpression or interference vectors. Cell proliferation was detected using a Cell Counting Kit‑8 assay. Cell migration and invasion were examined using a Transwell assay, while apoptosis, cell cycle and reactive oxygen species production were evaluated using flow cytometry, and the expression levels of lnc‑NLC1‑C, microRNA (miR)‑383 and peroxiredoxin 3 (PRDX‑3) were measured using western blotting and RT‑qPCR. Rescue experiments were performed to verify the function of the lnc‑NLC1‑C/miR‑383/PRDX‑3 axis. The highest expression levels of lnc‑NLC1‑C were identified in U87MG glioma cells. Overexpression of lnc‑NLC1‑C expression promoted cell proliferation, G1 phase blocking, migration and invasion, while inhibiting apoptosis and autophagy in U87MG cells. Mechanistically, miR‑383 could bind to lnc‑NLC1‑C to regulate PRDX‑3 expression and improve its oncogenic effect. Rescue experiments confirmed that the lnc‑NLC1‑C/miR‑383/PRDX‑3 axis was involved in the molecular mechanism of glioma progression. Therefore, lnc‑NLC1‑C may be a tumor promoter that affects multiple biological functions, such as migration, invasion and autophagy, in glioma cells.

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