Long non‑coding RNA OIP5‑AS1 promotes the progression of esophageal cancer by regulating miR‑30a/VOPP1 expression

Xu,Jiajun; Chen,Zhixi; Fang,Zheng; Chen,Shixiong; Guo,Ying; Liu,Xianfeng; Chen,Kai; Chen,Shengjia

doi:10.3892/ol.2021.12912

Long non‑coding RNA OIP5‑AS1 promotes the progression of esophageal cancer by regulating miR‑30a/VOPP1 expression

Authors:
- Jiajun Xu
- Zhixi Chen
- Zheng Fang
- Shixiong Chen
- Ying Guo
- Xianfeng Liu
- Kai Chen
- Shengjia Chen
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Affiliations: Department of Cardiothoracic Surgery, Affiliated Jiujiang Hospital of Nanchang University, Jiujiang, Jianxi 332000, P.R. China, College of Pharmacy, Gannan Medical University, Ganzhou, Jianxi 341000, P.R. China
Published online on: July 9, 2021 https://doi.org/10.3892/ol.2021.12912
Article Number: 651
Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNAs (lncRNAs) serve an important role in the development of esophageal cancer (EC), which is the eighth most common type of cancer worldwide. lncRNA opa‑interacting protein 5 antisense transcript 1 (OIP5‑AS1) is associated with human malignancy. However, the biological roles of OIP5‑AS1 in the development of EC remain unclear. In the present study, transfection was conducted, and reverse transcription‑quantitative PCR and western blot analysis were used for the detection of mRNA and protein expression, respectively. Furthermore, dual‑luciferase reporter and RNA immunoprecipitation assays were used to study the interaction between miRNA and lncRNA or genes. The results revealed that OIP5‑AS1 expression in EC tissues and cultured EC cells was upregulated, microRNA‑30a (miR‑30a) expression was downregulated. OIP5‑AS1‑knockdown suppressed the proliferation, migration and invasion of EC9706 and EC109 cells. miR‑30a was confirmed to interact with OIP5‑AS1, and miR‑30a‑mimics transfection ameliorated the effects of OIP5‑AS1 in EC cells. Vesicular overexpressed in cancer prosurvival protein 1 (VOPP1) was verified as the direct target of miR‑30a. VOPP1 expression was positively correlated with OIP5‑AS1 expression in EC cells. Overexpression of VOPP1 ameliorated the negative effects of OIP5‑AS1‑knockdown on EC9706 and EC109 cells. In conclusion, OIP5‑AS1 promoted the proliferation, migration and invasion of EC cells by increasing VOPP1 expression by sponging miR‑30a.

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