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Long non‑coding RNA OIP5‑AS1 promotes the progression of esophageal cancer by regulating miR‑30a/VOPP1 expression

  • Authors:
    • Jiajun Xu
    • Zhixi Chen
    • Zheng Fang
    • Shixiong Chen
    • Ying Guo
    • Xianfeng Liu
    • Kai Chen
    • Shengjia Chen
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, Affiliated Jiujiang Hospital of Nanchang University, Jiujiang, Jianxi 332000, P.R. China, College of Pharmacy, Gannan Medical University, Ganzhou, Jianxi 341000, P.R. China
    Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 651
    |
    Published online on: July 9, 2021
       https://doi.org/10.3892/ol.2021.12912
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Abstract

Long non‑coding RNAs (lncRNAs) serve an important role in the development of esophageal cancer (EC), which is the eighth most common type of cancer worldwide. lncRNA opa‑interacting protein 5 antisense transcript 1 (OIP5‑AS1) is associated with human malignancy. However, the biological roles of OIP5‑AS1 in the development of EC remain unclear. In the present study, transfection was conducted, and reverse transcription‑quantitative PCR and western blot analysis were used for the detection of mRNA and protein expression, respectively. Furthermore, dual‑luciferase reporter and RNA immunoprecipitation assays were used to study the interaction between miRNA and lncRNA or genes. The results revealed that OIP5‑AS1 expression in EC tissues and cultured EC cells was upregulated, microRNA‑30a (miR‑30a) expression was downregulated. OIP5‑AS1‑knockdown suppressed the proliferation, migration and invasion of EC9706 and EC109 cells. miR‑30a was confirmed to interact with OIP5‑AS1, and miR‑30a‑mimics transfection ameliorated the effects of OIP5‑AS1 in EC cells. Vesicular overexpressed in cancer prosurvival protein 1 (VOPP1) was verified as the direct target of miR‑30a. VOPP1 expression was positively correlated with OIP5‑AS1 expression in EC cells. Overexpression of VOPP1 ameliorated the negative effects of OIP5‑AS1‑knockdown on EC9706 and EC109 cells. In conclusion, OIP5‑AS1 promoted the proliferation, migration and invasion of EC cells by increasing VOPP1 expression by sponging miR‑30a.
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Copy and paste a formatted citation
Spandidos Publications style
Xu J, Chen Z, Fang Z, Chen S, Guo Y, Liu X, Chen K and Chen S: Long non‑coding RNA OIP5‑AS1 promotes the progression of esophageal cancer by regulating miR‑30a/VOPP1 expression. Oncol Lett 22: 651, 2021.
APA
Xu, J., Chen, Z., Fang, Z., Chen, S., Guo, Y., Liu, X. ... Chen, S. (2021). Long non‑coding RNA OIP5‑AS1 promotes the progression of esophageal cancer by regulating miR‑30a/VOPP1 expression. Oncology Letters, 22, 651. https://doi.org/10.3892/ol.2021.12912
MLA
Xu, J., Chen, Z., Fang, Z., Chen, S., Guo, Y., Liu, X., Chen, K., Chen, S."Long non‑coding RNA OIP5‑AS1 promotes the progression of esophageal cancer by regulating miR‑30a/VOPP1 expression". Oncology Letters 22.3 (2021): 651.
Chicago
Xu, J., Chen, Z., Fang, Z., Chen, S., Guo, Y., Liu, X., Chen, K., Chen, S."Long non‑coding RNA OIP5‑AS1 promotes the progression of esophageal cancer by regulating miR‑30a/VOPP1 expression". Oncology Letters 22, no. 3 (2021): 651. https://doi.org/10.3892/ol.2021.12912
Copy and paste a formatted citation
x
Spandidos Publications style
Xu J, Chen Z, Fang Z, Chen S, Guo Y, Liu X, Chen K and Chen S: Long non‑coding RNA OIP5‑AS1 promotes the progression of esophageal cancer by regulating miR‑30a/VOPP1 expression. Oncol Lett 22: 651, 2021.
APA
Xu, J., Chen, Z., Fang, Z., Chen, S., Guo, Y., Liu, X. ... Chen, S. (2021). Long non‑coding RNA OIP5‑AS1 promotes the progression of esophageal cancer by regulating miR‑30a/VOPP1 expression. Oncology Letters, 22, 651. https://doi.org/10.3892/ol.2021.12912
MLA
Xu, J., Chen, Z., Fang, Z., Chen, S., Guo, Y., Liu, X., Chen, K., Chen, S."Long non‑coding RNA OIP5‑AS1 promotes the progression of esophageal cancer by regulating miR‑30a/VOPP1 expression". Oncology Letters 22.3 (2021): 651.
Chicago
Xu, J., Chen, Z., Fang, Z., Chen, S., Guo, Y., Liu, X., Chen, K., Chen, S."Long non‑coding RNA OIP5‑AS1 promotes the progression of esophageal cancer by regulating miR‑30a/VOPP1 expression". Oncology Letters 22, no. 3 (2021): 651. https://doi.org/10.3892/ol.2021.12912
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