Low LINC01272 predicts poor prognosis of non‑small cell lung cancer and its biological function in tumor cells by inhibiting miR‑1303

Zhang,Sue; Zhou,Jielu

doi:10.3892/ol.2021.12913

Low LINC01272 predicts poor prognosis of non‑small cell lung cancer and its biological function in tumor cells by inhibiting miR‑1303

Authors:
- Sue Zhang
- Jielu Zhou
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Affiliations: Anesthesiology Department, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China
Published online on: July 9, 2021 https://doi.org/10.3892/ol.2021.12913
Article Number: 652
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Non‑small cell lung cancer (NSCLC) is a malignant tumor associated with poor prognosis. The clinical value of long non‑coding RNAs (lncRNAs) in the pathomechanism of various types of human malignancy has attracted increasing attention. The present study aimed to investigate the expression of LINC01272 in NSCLC and to determine its prognostic value and biological role. Tumor and adjacent non‑tumor tissues from 108 patients with NSCLC and NSCLC cell lines were used in this study. The expression levels of LINC01272 and microRNA (miR)‑1303 in tissues of patients and NSCLC cell lines were evaluated by reverse transcription quantitative PCR. The relationship between LINC01272 and the overall survival of patients with NSCLC was analyzed by Kaplan‑Meier survival curve and log‑rank test. Cox regression analysis confirmed the prognostic value of LINC01272 in patients with NSCLC. Cell Counting Kit‑8 assay was used to evaluate the proliferation of NSCLC cells. The migration and invasion of NSCLC cells were determined using Transwell assays. The interaction between LINC01272 and miR‑1303 in NSCLC was confirmed by dual‑luciferase reporter assay. LINC01272 downregulation in NSCLC tissues was associated with worse overall survival in patients based on bioinformatics analysis. Furthermore, LINC01272 expression, which was decreased in NSCLC tumor tissues and NSCLC cells, was considered as an independent prognostic biomarker in NSCLC. In addition, LINC01272 overexpression inhibited NSCLC cell proliferation, migration and invasion. miR‑1303 expression, which was increased in tumor tissues, was sponged by LINC01272 and negatively correlated with LINC01272 expression. miR‑1303 expression reversed the inhibitory effects of LINC01272 on NSCLC cell function. In summary, the findings from this study suggested that LINC01272 expression, which was decreased in NSCLC tumor tissues and NSCLC cells, may be used as an independent prognostic biomarker for patients with NSCLC and that its overexpression may suppress NSCLC cell proliferation, migration and invasion by inhibiting miR‑1303.

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