Effect and mechanism of action of SLP‑2 on the apoptosis and autophagy of gastric cancer cells

Yang,Shengsen; Huang,Yun; Zhang,Hongyan; Wang,Fang; Shao,Liangui; Wang,Xuehong

doi:10.3892/ol.2021.12968

Effect and mechanism of action of SLP‑2 on the apoptosis and autophagy of gastric cancer cells

Authors:
- Shengsen Yang
- Yun Huang
- Hongyan Zhang
- Fang Wang
- Liangui Shao
- Xuehong Wang
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Affiliations: Department of Gastroenterology, Affiliated Hospital of Qinghai University, Xining, Qianghai 810001, P.R. China
Published online on: August 4, 2021 https://doi.org/10.3892/ol.2021.12968
Article Number: 707
Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

This study was designed to investigate the effect of stomatin‑like protein 2 (SLP‑2) on the apoptosis and autophagy of gastric cancer cells and its underlying mechanism. The expression of SLP‑2 was detected in human gastric cancer cell lines (AGS, MKN‑45 and NCI‑N87) and a human gastric epithelial cell line (GES‑1) using reverse transcription‑quantitative PCR and western blot analysis. SLP‑2‑specific small interfering RNA (siRNA) was transfected into NCI‑N87 cells. Cell Counting Kit‑8 was used to detect cell proliferation. Apoptosis rates were measured using flow cytometry. Autophagosomes were observed by transmission electron microscopy. The expression levels of Annexin A2 (ANXA2), β‑catenin, Bcl‑2, Bax, Beclin‑1 and LC3‑II/I were also measured. The results demonstrated that SLP‑2 siRNA transfection significantly reduced cell proliferation and increased cell apoptosis. The mitochondria were severely damaged, and a large number of autophagosomes were seen in SLP‑2 siRNA‑transfected NCI‑N87 cells. Furthermore, the expression levels of ANXA2, β‑catenin and Bcl‑2 were downregulated, whereas those of Bax, Beclin‑1 and LC3‑II/I were upregulated following SLP‑2 siRNA transfection. In conclusion, SLP‑2 silencing can inhibit proliferation and induce apoptosis and autophagy of gastric cancer cells, and this effect may be related to the inhibition of ANXA2/β‑catenin signaling pathway.

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