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Article

FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells

  • Authors:
    • Chengfei Jiang
    • Binbin Yuan
    • Bo Hang
    • Jian-Hua Mao
    • Xiaoping Zou
    • Pin Wang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA
  • Article Number: 712
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    Published online on: August 5, 2021
       https://doi.org/10.3892/ol.2021.12973
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Abstract

Gastric cancer (GC) is one of the main causes of cancer‑associated morbidity and mortality worldwide. The present study aimed to investigate the role of the gene encoding formin homology 2 domain containing 1 (FHOD1) protein in GC development. Data from The Cancer Genome Atlas were firstly analyzed, and immunohistochemistry was conducted on GC tissues. The results demonstrated that FHOD1 expression in GC tissues was significantly increased compared with adjacent non‑tumor tissues. Furthermore, the expression level of FHOD1 was negatively associated with the overall survival of patients with GC. For the functional studies, lentivirus‑mediated short hairpin RNA against FHOD1 and FHOD1‑overexpression vectors were constructed to knockdown and overexpress the expression level of FHOD1 in human GC cell lines, respectively. The results indicated that FHOD1 knockdown inhibited the proliferation, colony formation and migratory and invasive abilities of GC cells. Conversely, overexpression of FHOD1 in GC cells promoted soft‑agar colony formation and migratory and invasive abilities. In addition, it was demonstrated that genes of which expression levels were correlated with FHOD1 were enriched in the Gene Ontology term of ‘extracellular matrix (ECM) structural constituent', suggesting that FHOD1 may serve an important role in the regulation of ECM. In conclusion, the present study demonstrated that FHOD1 may exert an oncogenic role in cultured GC cells and be inversely associated with the overall survival of patients with GC.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang C, Yuan B, Hang B, Mao J, Zou X and Wang P: FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells. Oncol Lett 22: 712, 2021.
APA
Jiang, C., Yuan, B., Hang, B., Mao, J., Zou, X., & Wang, P. (2021). FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells. Oncology Letters, 22, 712. https://doi.org/10.3892/ol.2021.12973
MLA
Jiang, C., Yuan, B., Hang, B., Mao, J., Zou, X., Wang, P."FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells". Oncology Letters 22.4 (2021): 712.
Chicago
Jiang, C., Yuan, B., Hang, B., Mao, J., Zou, X., Wang, P."FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells". Oncology Letters 22, no. 4 (2021): 712. https://doi.org/10.3892/ol.2021.12973
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang C, Yuan B, Hang B, Mao J, Zou X and Wang P: FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells. Oncol Lett 22: 712, 2021.
APA
Jiang, C., Yuan, B., Hang, B., Mao, J., Zou, X., & Wang, P. (2021). FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells. Oncology Letters, 22, 712. https://doi.org/10.3892/ol.2021.12973
MLA
Jiang, C., Yuan, B., Hang, B., Mao, J., Zou, X., Wang, P."FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells". Oncology Letters 22.4 (2021): 712.
Chicago
Jiang, C., Yuan, B., Hang, B., Mao, J., Zou, X., Wang, P."FHOD1 is upregulated in gastric cancer and promotes the proliferation and invasion of gastric cancer cells". Oncology Letters 22, no. 4 (2021): 712. https://doi.org/10.3892/ol.2021.12973
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