Open Access

Metformin mitigates PLCε gene expression and modulates the Notch1/Hes and androgen receptor signaling pathways in castration‑resistant prostate cancer xenograft models

  • Authors:
    • Qi Li
    • Ke Xu
    • Jianguo Tian
    • Zhicheng Lu
    • Jianming Pu
  • View Affiliations

  • Published online on: August 8, 2021     https://doi.org/10.3892/ol.2021.12976
  • Article Number: 715
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study aimed to establish a mouse model of patient‑derived castration‑resistant prostate cancer (CRPC) xenograft tumors, and to evaluate the effects of various doses of metformin on phospholipase Cε (PLCε) expression and the neurogenic locus notch homolog protein 1 (Notch1)/hairy and enhancer of split 1 and androgen receptor (AR) signaling pathways via western blotting and reverse transcription‑quantitative PCR. Additionally, phorbol 12‑myristate 13‑acetate was used to activate PLC, and Jagged1 was used as a Notch activator to verify whether metformin could suppress CRPC development via the PLCε/Notch1/AR pathways. The results confirmed that metformin may serve critical roles in CRPC by significantly inhibiting the occurrence, growth and proliferation of CRPC tumors by decreasing PLCε/Notch1 expression and AR nucleation.
View Figures
View References

Related Articles

Journal Cover

October-2021
Volume 22 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Li Q, Xu K, Tian J, Lu Z and Pu J: Metformin mitigates PLCε gene expression and modulates the Notch1/Hes and androgen receptor signaling pathways in castration‑resistant prostate cancer xenograft models. Oncol Lett 22: 715, 2021
APA
Li, Q., Xu, K., Tian, J., Lu, Z., & Pu, J. (2021). Metformin mitigates PLCε gene expression and modulates the Notch1/Hes and androgen receptor signaling pathways in castration‑resistant prostate cancer xenograft models. Oncology Letters, 22, 715. https://doi.org/10.3892/ol.2021.12976
MLA
Li, Q., Xu, K., Tian, J., Lu, Z., Pu, J."Metformin mitigates PLCε gene expression and modulates the Notch1/Hes and androgen receptor signaling pathways in castration‑resistant prostate cancer xenograft models". Oncology Letters 22.4 (2021): 715.
Chicago
Li, Q., Xu, K., Tian, J., Lu, Z., Pu, J."Metformin mitigates PLCε gene expression and modulates the Notch1/Hes and androgen receptor signaling pathways in castration‑resistant prostate cancer xenograft models". Oncology Letters 22, no. 4 (2021): 715. https://doi.org/10.3892/ol.2021.12976