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Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid

  • Authors:
    • Ana C. Luque‑Badillo
    • Griselda Hernandez‑Tapia
    • Dhamar A. Ramirez‑Castillo
    • Diego Espinoza‑Serrano
    • Alan M. Cortes‑Limon
    • Juan P. Cortes‑Gallardo
    • Daniel A. Jacobo‑Velázquez
    • Margarita L. Martinez‑Fierro
    • Clara P. Rios‑Ibarra
  • View Affiliations / Copyright

    Affiliations: School of Engineering and Sciences, Tecnologico de Monterrey, Campus Guadalajara, Jalisco 45138, Mexico, Molecular Medicine Laboratory, Academic Unit of Human Medicine and Health Sciences, Universidad Autónoma de Zacatecas, Zacatecas 98040, Mexico
    Copyright: © Luque‑Badillo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 742
    |
    Published online on: August 19, 2021
       https://doi.org/10.3892/ol.2021.13003
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Abstract

In the present study, the inhibitory effect of chlorogenic acid (CGA), a phenolic compound with potential antitumor effects, on circulating microRNA 31 (miR‑31), was evaluated in RKO colon cancer cells. The capacity of gold nanoparticles (AuNPs) to enhance miR‑31 quantification after treatment with CGA was assessed. RKO cells were treated with different concentrations of CGA for 24, 48 and 72 h, after which AuNPs coupled to CD81 were added to the supernatants. Total RNA was extracted, and miR‑31 was quantified by reverse transcription‑quantitative PCR. The results revealed an 85% decrease in miR‑31 level following treatment with 1,000 µM CGA for 72 h, and the highest capacity to detect miR‑31 (after treatment and isolation with AuNPs + CD81) was observed at 24 h. Furthermore, CGA decreased the expression of the miR‑31 oncogene in an in vitro colon cancer model, and the use of AuNPs enhanced the levels of miRNA detection. The results suggest that miR‑31 inhibition is one mechanism by which CGA decreases colon cancer cell proliferation. Moreover, AuNPs can increase the capacity of miR‑31 quantification, representing a new strategy to develop non‑invasive tools for the molecular diagnosis and monitoring of colon cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Luque‑Badillo AC, Hernandez‑Tapia G, Ramirez‑Castillo DA, Espinoza‑Serrano D, Cortes‑Limon AM, Cortes‑Gallardo JP, Jacobo‑Velázquez DA, Martinez‑Fierro ML and Rios‑Ibarra CP: Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid<em></em>. Oncol Lett 22: 742, 2021.
APA
Luque‑Badillo, A.C., Hernandez‑Tapia, G., Ramirez‑Castillo, D.A., Espinoza‑Serrano, D., Cortes‑Limon, A.M., Cortes‑Gallardo, J.P. ... Rios‑Ibarra, C.P. (2021). Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid<em></em>. Oncology Letters, 22, 742. https://doi.org/10.3892/ol.2021.13003
MLA
Luque‑Badillo, A. C., Hernandez‑Tapia, G., Ramirez‑Castillo, D. A., Espinoza‑Serrano, D., Cortes‑Limon, A. M., Cortes‑Gallardo, J. P., Jacobo‑Velázquez, D. A., Martinez‑Fierro, M. L., Rios‑Ibarra, C. P."Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid<em></em>". Oncology Letters 22.4 (2021): 742.
Chicago
Luque‑Badillo, A. C., Hernandez‑Tapia, G., Ramirez‑Castillo, D. A., Espinoza‑Serrano, D., Cortes‑Limon, A. M., Cortes‑Gallardo, J. P., Jacobo‑Velázquez, D. A., Martinez‑Fierro, M. L., Rios‑Ibarra, C. P."Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid<em></em>". Oncology Letters 22, no. 4 (2021): 742. https://doi.org/10.3892/ol.2021.13003
Copy and paste a formatted citation
x
Spandidos Publications style
Luque‑Badillo AC, Hernandez‑Tapia G, Ramirez‑Castillo DA, Espinoza‑Serrano D, Cortes‑Limon AM, Cortes‑Gallardo JP, Jacobo‑Velázquez DA, Martinez‑Fierro ML and Rios‑Ibarra CP: Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid<em></em>. Oncol Lett 22: 742, 2021.
APA
Luque‑Badillo, A.C., Hernandez‑Tapia, G., Ramirez‑Castillo, D.A., Espinoza‑Serrano, D., Cortes‑Limon, A.M., Cortes‑Gallardo, J.P. ... Rios‑Ibarra, C.P. (2021). Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid<em></em>. Oncology Letters, 22, 742. https://doi.org/10.3892/ol.2021.13003
MLA
Luque‑Badillo, A. C., Hernandez‑Tapia, G., Ramirez‑Castillo, D. A., Espinoza‑Serrano, D., Cortes‑Limon, A. M., Cortes‑Gallardo, J. P., Jacobo‑Velázquez, D. A., Martinez‑Fierro, M. L., Rios‑Ibarra, C. P."Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid<em></em>". Oncology Letters 22.4 (2021): 742.
Chicago
Luque‑Badillo, A. C., Hernandez‑Tapia, G., Ramirez‑Castillo, D. A., Espinoza‑Serrano, D., Cortes‑Limon, A. M., Cortes‑Gallardo, J. P., Jacobo‑Velázquez, D. A., Martinez‑Fierro, M. L., Rios‑Ibarra, C. P."Gold nanoparticles enhance microRNA 31 detection in colon cancer cells after inhibition with chlorogenic acid<em></em>". Oncology Letters 22, no. 4 (2021): 742. https://doi.org/10.3892/ol.2021.13003
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