Expression of oncogenic long noncoding RNA PSMG3‑antisense 1 in lung squamous cell carcinoma

Jin,E; Huang,Chao; Zhang,Lei; Chen,Shiyi; Zhao,Xiaochen; Ren,Zheng; Fu,Hong

doi:10.3892/ol.2021.13012

Expression of oncogenic long noncoding RNA PSMG3‑antisense 1 in lung squamous cell carcinoma

Authors:
- E Jin
- Chao Huang
- Lei Zhang
- Shiyi Chen
- Xiaochen Zhao
- Zheng Ren
- Hong Fu
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Affiliations: Department of Medical Oncology, The Fourth People's Hospital of Shenyang, Shenyang, Liaoning 110031, P.R. China, Department of Interventional Oncology, The Fourth People's Hospital of Shenyang, Shenyang, Liaoning 110031, P.R. China
Published online on: August 27, 2021 https://doi.org/10.3892/ol.2021.13012
Article Number: 751

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Abstract

Lung squamous cell carcinoma (LUSC) is one of the most common subtypes of lung cancer that accounts for ~50% of all lung cancer cases. Long noncoding RNA (lncRNA) PSMG3‑antisense (AS) 1 has been suggested to play an important role in various types of cancer. Therefore, the aim of the present study was to investigate the role of PSMG3‑AS1 using clinical specimens and data from 130 patients with LUSC. The expression levels of PSMG3‑AS1 and miR‑143‑3p were detected in LUSC specimens, and the correlation between lncRNA PSMG3‑AS1 expression and patient clinical characteristics was analyzed. Cell Counting Kit‑8, Transwell migration and invasion assays were used to investigate the functional role of PSMG3‑AS1 in LUSC. The mechanism of PSMG3‑AS1 on LUSC cells was also investigated using a luciferase activity assay with wild‑type or mutated PSMG3‑AS1. PSMG3‑AS1 was found to be upregulated in LUSC, and high expression was associated with positive lymph node metastasis and a higher TNM stage. The results of multivariate Cox regression analysis revealed that PSMG3‑AS1 may serve as an independent prognostic indicator in LUSC. Furthermore, inhibiting PSMG3‑AS1 expression reduced tumor cell proliferative, migratory and invasive abilities. Moreover, PSMG3‑AS1 was found to be closely associated with miR‑143‑3p in LUSC, and thus may become a potential prognostic marker and therapeutic target for the treatment of LUSC in the future.

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