UCHL3 plays an important role in the occurrence and development of melanoma

He,Runzhi; Zhou,Yajing; Liu,Jianmin; Zhang,Xiaochong; Zhao,Xiaoling; An,Lihui; Li,Zihan; Cheng,Fang

doi:10.3892/ol.2021.13017

UCHL3 plays an important role in the occurrence and development of melanoma

Authors:
- Runzhi He
- Yajing Zhou
- Jianmin Liu
- Xiaochong Zhang
- Xiaoling Zhao
- Lihui An
- Zihan Li
- Fang Cheng
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Affiliations: The Third Department of Neurosurgery, Xingtai People's Hospital, Xingtai, Hebei 054001, P.R. China, Department of Anesthesiology, Xingtai People's Hospital, Xingtai, Hebei 054001, P.R. China, Department of Orthopedics, Xingtai People's Hospital, Xingtai, Hebei 054001, P.R. China, Science and Education Section, Xingtai People's Hospital, Xingtai, Hebei 054001, P.R. China, Tumor Laboratory, Xingtai People's Hospital, Xingtai, Hebei 054001, P.R. China, Department of Dermatology, Xingtai People's Hospital, Xingtai, Hebei 054001, P.R. China
Published online on: September 1, 2021 https://doi.org/10.3892/ol.2021.13017
Article Number: 756
Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

It has been reported that ubiquitin C‑terminal hydrolase‑L3 (UCHL3) plays an important role in cancer development; however, the role of UCHL3 in melanoma remains unclear. The present study aimed to investigate the role of ubiquitin C‑terminal hydrolase‑L3 (UCHL3) and determine its underlying molecular mechanisms in melanoma occurrence and development using in vitro studies. Reverse transcription‑quantitative PCR analysis was performed to detect UCHL3 mRNA expression. The MTT assay was performed to assess cell proliferation. Cell apoptosis was analyzed via flow cytometry and the TUNEL assay. Cell ultrastructure was observed via transmission electron microscopy. LC3B protein expression was detected via cellular immunofluorescence, while neural precursor cell‑expressed developmentally downregulated protein 8 (NEDD8) and LC3 protein expression levels, and NEDD8 ubiquitination were detected via western blot analysis. The results demonstrated that transfection with small interfering (si)RNA‑UCHL3 significantly suppressed cell proliferation, whereas apoptosis was significantly enhanced, as well as autophagy, autophagosome formation and LC3B protein expression. In addition, NEDD8 protein expression and autophagosome numbers significantly decreased, while the LC3II/LC3I ratio significantly increased. NEDD8 knockdown via transfection with si‑NEDD8 had similar effects to si‑UCHL3, as well as si‑UCHL3+ si‑NEDD8. Taken together, the results of the present study suggest that UCHL3 knockdown decreases melanoma cell proliferation by increasing cell autophagy through regulating NEDD8 expression and autophagosome numbers.

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