MicroRNA‑545‑5p regulates apoptosis, migration and invasion of osteosarcoma by targeting dimethyladenosine transferase 1

Zhou,Hai-Zhen; Chen,Bo; Li,Xiao-Ju; Du,Juan-Juan; Zhang,Nan; Shao,Yu-Xiong; Zhang,Kun; Tong,Zhi-Chao

doi:10.3892/ol.2021.13024

MicroRNA‑545‑5p regulates apoptosis, migration and invasion of osteosarcoma by targeting dimethyladenosine transferase 1

Authors:
- Hai-Zhen Zhou
- Bo Chen
- Xiao-Ju Li
- Juan-Juan Du
- Nan Zhang
- Yu-Xiong Shao
- Kun Zhang
- Zhi-Chao Tong
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Affiliations: Department of Osteopathic Oncology, Honghui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710054, P.R. China
Published online on: September 6, 2021 https://doi.org/10.3892/ol.2021.13024
Article Number: 763
Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The metastasis of osteosarcoma is a major threat to both adolescents and young adults. Identifying novel targets that may prevent osteosarcoma metastasis is critical in developing advanced clinical therapies for treating this cancer. The present study aimed to explore the mechanism of microRNA (miR)‑545‑5p in the metastasis of osteosarcoma. The present study identified miR‑545‑5p as a potential target that was downregulated in both osteosarcoma clinical samples and cell lines, and in the latter, ectopically expressed miR‑545‑5p caused apoptosis. In addition, miR‑545‑5p exerted inhibitory effects in osteosarcoma migration and invasion. Overexpression of miR‑545‑5p induced xenograft growth inhibition in vivo. In addition, miR‑545‑5p targeted dimethyladenosine transferase 1 (DIMT1), an oncogenic protein that facilitates osteosarcoma proliferation, migration and invasion. Taken together, the results of the present study suggest that miR‑545‑5p functions as a tumor suppressor in osteosarcoma that promotes apoptosis, while inhibiting migration and invasion by targeting DIMT1. Taken together, the results of the present study suggest two potential novel targets for osteosarcoma treatment and metastasis prevention.

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