Long non‑coding RNA SNHG17 promotes lung adenocarcinoma progression by targeting the microRNA‑193a‑5p/NETO2 axis

Zhang,Zhiwei; Yan,Yulan; Zhang,Bin; Ma,Yuchen; Chen,Chen; Wang,Changli

doi:10.3892/ol.2021.13079

Long non‑coding RNA SNHG17 promotes lung adenocarcinoma progression by targeting the microRNA‑193a‑5p/NETO2 axis

Authors:
- Zhiwei Zhang
- Yulan Yan
- Bin Zhang
- Yuchen Ma
- Chen Chen
- Changli Wang
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Affiliations: Department of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin 300060, P.R. China, Department of Thoracic Surgery, The Fifth Central Hospital of Tianjin, Tianjin 300450, P.R. China
Published online on: October 5, 2021 https://doi.org/10.3892/ol.2021.13079
Article Number: 818
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNAs (lncRNAs) play vital roles in human cancers. It has been reported that lncRNA SNHG17 expression is dysregulated in different types of cancer and involved in cancer progression. However, the role of SNHG17 in lung adenocarcinoma (LUAD) remains unclear. The present study aimed to investigate the role of SNHG17 in LUAD. Reverse transcription‑quantitative (RT‑q) PCR analysis was performed to detect SNHG17 expression in LUAD tissues and cells. The effects of SNHG17 on cancer cell migration, invasion, proliferation and epithelial‑to‑mesenchymal transition (EMT) were assessed via Transwell, MTT and western blot assays, respectively. The interactions between SNHG17 and microRNA (miRNA/miR)‑193a‑5p, miR‑193a‑5p and neuropilin and tolloid‑like 2 (NETO2) were assessed via the dual‑luciferase reporter assay. NETO2 expression and its potential role in LUAD were analyzed via RT‑qPCR analysis and the UALCAN database. The results demonstrated that SNHG17 expression was significantly upregulated in LUAD tissues and cells, and high SNHG17 expression was associated with tumor‑node‑metastasis stage and poor prognosis of patients with LUAD. SNHG17 knockdown inhibited cell migration, invasion, proliferation and the EMT process. In addition, the results revealed that SNHG17 functions as a competing endogenous RNA of miR‑193a‑5p. The results of the dual‑luciferase reporter assay confirmed that miR‑193a‑5p can directly target SNHG17. NETO2 was also predicted as a target protein of miR‑193a‑5p, which was confirmed via the dual‑luciferase reporter assay. The roles of NETO2 knockdown in cancer cells were rescued following transfection with miR‑193a‑5p inhibitor or overexpression of SNHG17. Notably, high NETO2 expression was associated with poor prognosis of patients with LUAD. Bioinformatics analysis demonstrated that the promoter methylation level of NETO2 decreased in LUAD. Taken together, the results of the present study suggest that SNHG17 expression is upregulated in LUAD tissues and cells, and SNHG17 exerts tumor promoting effect by targeting the miR‑193a‑5p/NETO2 axis.

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