Delphinidin induces cell cycle arrest and apoptosis in HER‑2 positive breast cancer cell lines by regulating the NF‑κB and MAPK signaling pathways

Wu,Ailin; Zhu,Yanfeng; Han,Bin; Peng,Jiayuan; Deng,Xiaomin; Chen,Wei; Du,Jingchang; Ou,Yu; Peng,Xiaoli; Yu,Xiaoping

doi:10.3892/ol.2021.13093

Delphinidin induces cell cycle arrest and apoptosis in HER‑2 positive breast cancer cell lines by regulating the NF‑κB and MAPK signaling pathways

Authors:
- Ailin Wu
- Yanfeng Zhu
- Bin Han
- Jiayuan Peng
- Xiaomin Deng
- Wei Chen
- Jingchang Du
- Yu Ou
- Xiaoli Peng
- Xiaoping Yu
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Affiliations: Basic Medical School, Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China, Graduate School, Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China, School of Public Health, Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China, Division of Planning and Finance, Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China
Published online on: October 14, 2021 https://doi.org/10.3892/ol.2021.13093
Article Number: 832
Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Delphinidin is an anthocyanidin monomer, commonly found in vegetables and fruits, and has demonstrated antitumor effects in the HER‑2‑positive MDA‑MB‑453 breast cancer cell line, with low cytotoxicity on normal breast cells. However, the direct functional mechanisms underlying the effect of delphinidin on HER‑2‑positive breast cancer cells has not been fully characterized. In the present study, it was found that delphinidin could induce G2/M phase cell cycle arrest by inhibiting the protein expression level of cyclin B1 and Cdk1 in HER‑2‑positive breast cancer cell lines. In addition, delphinidin promoted the mitochondrial apoptosis pathway by inhibiting the ERK and NF‑κB signaling pathway and activating the JNK signaling pathway. Therefore, delphinidin markedly suppressed the viability of the HER‑2‑positive breast cancer cell lines by modulating the cell cycle and inducing apoptosis. Overall, the findings from the present study demonstrated that delphinidin treatment could induce the mitochondrial apoptosis pathway in human HER‑2‑positive breast cancer cell lines, providing an experimental basis for the prevention and treatment of HER‑2‑positive breast cancer by flavonoids.

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