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miR‑152‑mediated MKK7 downregulation is attenuated by MYCNOS in ovarian adenocarcinoma

  • Authors:
    • Guifang Zhang
    • Dan Zheng
    • Xiaoqing Chen
    • Li Li
    • Jingrong Yu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330008, P.R. China, Day Operation Center, Jiangxi Maternal and Child Health Hospital, Nanchang, Jiangxi 330003, P.R. China, Department of Oncology, The Fourth Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330003, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 841
    |
    Published online on: October 20, 2021
       https://doi.org/10.3892/ol.2021.13102
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Abstract

MYCN opposite strand (MYCNOS) acts as an oncogenic long non‑coding RNA in liver cancer. However, its role in other cancer types is unknown. The aim of the present study was to investigate the function of MYCNOS in ovarian adenocarcinoma (OA). MYCNOS expression in OA was determined using reverse transcription‑quantitative PCR (RT‑qPCR), and its prognostic value for OA was evaluated in a 5‑year follow‑up study. The predicted interaction between MYCNOS and microRNA (miR)‑152 was confirmed using a dual luciferase reporter assay. The association between MYCNOS and miR‑152 was also analyzed in overexpression experiments. The effects of MYCNOS and miR‑152 on mitogen‑activated protein kinase kinase 7 (MKK7) expression were explored using RT‑qPCR and western blotting. Cell proliferation was analyzed using a Cell Counting Kit‑8 assay. MYCNOS expression was found to be upregulated in OA and predicted poor survival. In addition, MYCNOS was predicted to interact with miR‑152, and a dual luciferase assay confirmed this interaction. However, MYCNOS and miR‑152 overexpression did not affect their mutual expression levels. MYCNOS overexpression upregulated MKK7, a target of miR‑152. Cell proliferation increased following simultaneous MYCNOS and MKK7 overexpression, but was reduced following miR‑152 overexpression. Moreover, MYCNOS overexpression attenuated the effects of miR‑152 overexpression. In conclusion, MYCNOS may act by sponging miR‑152 to upregulate MKK7 expression in OA, thereby promoting cell proliferation.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang G, Zheng D, Chen X, Li L and Yu J: miR‑152‑mediated MKK7 downregulation is attenuated by MYCNOS in ovarian adenocarcinoma. Oncol Lett 22: 841, 2021.
APA
Zhang, G., Zheng, D., Chen, X., Li, L., & Yu, J. (2021). miR‑152‑mediated MKK7 downregulation is attenuated by MYCNOS in ovarian adenocarcinoma. Oncology Letters, 22, 841. https://doi.org/10.3892/ol.2021.13102
MLA
Zhang, G., Zheng, D., Chen, X., Li, L., Yu, J."miR‑152‑mediated MKK7 downregulation is attenuated by MYCNOS in ovarian adenocarcinoma". Oncology Letters 22.6 (2021): 841.
Chicago
Zhang, G., Zheng, D., Chen, X., Li, L., Yu, J."miR‑152‑mediated MKK7 downregulation is attenuated by MYCNOS in ovarian adenocarcinoma". Oncology Letters 22, no. 6 (2021): 841. https://doi.org/10.3892/ol.2021.13102
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang G, Zheng D, Chen X, Li L and Yu J: miR‑152‑mediated MKK7 downregulation is attenuated by MYCNOS in ovarian adenocarcinoma. Oncol Lett 22: 841, 2021.
APA
Zhang, G., Zheng, D., Chen, X., Li, L., & Yu, J. (2021). miR‑152‑mediated MKK7 downregulation is attenuated by MYCNOS in ovarian adenocarcinoma. Oncology Letters, 22, 841. https://doi.org/10.3892/ol.2021.13102
MLA
Zhang, G., Zheng, D., Chen, X., Li, L., Yu, J."miR‑152‑mediated MKK7 downregulation is attenuated by MYCNOS in ovarian adenocarcinoma". Oncology Letters 22.6 (2021): 841.
Chicago
Zhang, G., Zheng, D., Chen, X., Li, L., Yu, J."miR‑152‑mediated MKK7 downregulation is attenuated by MYCNOS in ovarian adenocarcinoma". Oncology Letters 22, no. 6 (2021): 841. https://doi.org/10.3892/ol.2021.13102
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