Open Access

Inhibition of p38 MAPK increases the sensitivity of 5‑fluorouracil‑resistant SW480 human colon cancer cells to noscapine

  • Authors:
    • Zheng Han
    • Liu Meng
    • Xiaodong Huang
    • Jie Tan
    • Weijie Liu
    • Wei Chen
    • Yanli Zou
    • Yishan Cai
    • Shasha Huang
    • Aifang Chen
    • Ting Zhan
    • Min Huang
    • Xiaoli Chen
    • Xia Tian
    • Qingxi Zhu
  • View Affiliations

  • Published online on: December 17, 2021     https://doi.org/10.3892/ol.2021.13170
  • Article Number: 52
  • Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A major cause of treatment failure in advanced colon cancer is resistance to chemotherapy. p38 mitogen‑activated protein kinase (MAPK) has been associated with cellular apoptosis and plays an important role in multidrug resistance (MDR) in cancer cells. In the present study the effect of p38 MAPK on the sensitivity of 5‑fluorouracil (5‑FU)‑resistant SW480 (SW480/5‑FU) human colon cancer cells to noscapine was investigated. Following p38 MAPK interference, the inhibitory effect of noscapine on cell viability and proliferation was increased in the SW480/5‑FU cells and there was also a decrease in the expression level of minichromosome maintenance proteins, recombinant Ki‑67 and proliferating cell nuclear antigen. Inhibition of p38 MAPK also enhanced noscapine‑induced G1‑phase cell cycle arrest in the SW480/5‑FU cells and there was also a decrease in the protein and mRNA expression level of cyclin D, cyclin E and cyclin‑dependent kinase 2, and an increase in the expression level of P57. Furthermore, p38 MAPK interference increased noscapine‑induced apoptosis of the SW480/5‑FU cells and there was an increase in the protein and mRNA expression level of caspases‑3 and 8 and Bax, and decreased Bcl‑2 expression level. The sensitivity of the SW480/5‑FU cells to noscapine was also increased following p38 MAPK interference, as demonstrated by MDR inhibition via decreased Akt activity and reduced protein expression level of the MDR proteins P‑glycoprotein, multidrug resistance protein 1 and ATP‑binding cassette G2. These observations indicated that inhibition of p38 MAPK increased the sensitivity of the SW480/5‑FU cells to noscapine by suppressing proliferation, induction of cell cycle arrest and apoptosis, and reversal of MDR in the SW480/5‑FU cells.
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February-2022
Volume 23 Issue 2

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Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Han Z, Meng L, Huang X, Tan J, Liu W, Chen W, Zou Y, Cai Y, Huang S, Chen A, Chen A, et al: Inhibition of p38 MAPK increases the sensitivity of 5‑fluorouracil‑resistant SW480 human colon cancer cells to noscapine. Oncol Lett 23: 52, 2022
APA
Han, Z., Meng, L., Huang, X., Tan, J., Liu, W., Chen, W. ... Zhu, Q. (2022). Inhibition of p38 MAPK increases the sensitivity of 5‑fluorouracil‑resistant SW480 human colon cancer cells to noscapine. Oncology Letters, 23, 52. https://doi.org/10.3892/ol.2021.13170
MLA
Han, Z., Meng, L., Huang, X., Tan, J., Liu, W., Chen, W., Zou, Y., Cai, Y., Huang, S., Chen, A., Zhan, T., Huang, M., Chen, X., Tian, X., Zhu, Q."Inhibition of p38 MAPK increases the sensitivity of 5‑fluorouracil‑resistant SW480 human colon cancer cells to noscapine". Oncology Letters 23.2 (2022): 52.
Chicago
Han, Z., Meng, L., Huang, X., Tan, J., Liu, W., Chen, W., Zou, Y., Cai, Y., Huang, S., Chen, A., Zhan, T., Huang, M., Chen, X., Tian, X., Zhu, Q."Inhibition of p38 MAPK increases the sensitivity of 5‑fluorouracil‑resistant SW480 human colon cancer cells to noscapine". Oncology Letters 23, no. 2 (2022): 52. https://doi.org/10.3892/ol.2021.13170