circular RNAs 0000515 and 0011385 as potential biomarkers for disease monitoring and determining prognosis in pancreatic ductal adenocarcinoma
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- Published online on: December 21, 2021 https://doi.org/10.3892/ol.2021.13174
- Article Number: 56
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Copyright: © Wu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Pancreatic ductal adenocarcinoma (PDAC) is one of the most fatal cancers worldwide (1). The incidence of PDAC has continuously increased during the past two decades, and its incidence has been reported to have increased from 12.1 per 100,000 in 1997–2000 to 15.3 per 100,000 in 2013–2016 (2). It has been reported that PDAC is the second leading cause of cancer-associated mortality worldwide (3,4). Currently, the therapeutic approaches used to treat patients with PDAC are still based on surgical resection, traditional chemotherapy and radiation (5). Despite advancements in these treatment modalities, the survival rate of patients with PDAC remains unsatisfactory with 3-year survival ranging between 16.9 and 25.4% during 1997–2016 (2,6). Along with the development of molecular science, investigators are now focusing on the molecular genetics of PDAC to identify its subtypes and design precision medicine strategies (7,8). Currently, several genetic variations have been identified in PDAC, such as mutations in the KRAS, cyclin-dependent kinase inhibitor 2A and TP53 genes (9,10). However, the effects of these mutations have been mostly restricted to the research level (9,11). Thus, the application of these findings on treating patients with PDAC is yet to be implemented (7,8).
circular RNAs (circRNAs), characterized by the closed coil structure resulting from a lack of Poly A tail, are a class of small endogenous molecules (12). Despite recent advancements in circRNA research, the origin and roles of circRNAs remain unclear. Several biological functions of circRNAs have been established, such as regulating the structure of protein complexes and the expression of their original genes, and sponging microRNAs (miRNAs/miRs) (13–15). With regards to the role of circRNAs in oncology, microarray analyses have indicated that partial circRNAs are aberrantly expressed in different types of cancer, such as lung adenocarcinoma, squamous cell carcinoma and glioma, and involved in their initiation and progression (16–18). Notably, circRNAs exhibit a more stable structure compared with other types of non-coding RNAs in human tissues and cells (19); therefore, several studies have been performed to investigate the expression profiles and the potential functions of circRNAs in patients with cancer (20,21). Previously, two studies analyzed the circRNA expression profiles in patients with PDAC; however, the differentially expressed circRNAs (DEcircRNAs) identified in these studies lacked validation in large scale clinical studies, and their associations with clinical characteristics or survival have not yet been investigated (22,23).
Based on the two datasets from the previous studies, GSE69362 and GSE79634, the present study performed a secondary analysis to determine the candidate circRNAs by identifying the DEcircRNAs with accordant expression trends in the two datasets. A total of nine candidate circRNAs were identified and validated in tissue samples from 60 patients with PDAC via reverse transcription-quantitative (RT-q)PCR analysis. The present study also investigated the correlation of these candidate circRNAs with clinicopathological characteristics, and their association with survival of patients with PDAC.
Materials and methods
Microarray data collection and analysis
To identify the DEcircRNAs in PDAC, the circRNA expression profile datasets from the National Center of Biotechnology Information Gene Expression Omnibus database were searched (NCBI GEO, http://www.ncbi.nlm.nih.gov/geo). A total of two datasets, GSE69362 and GSE79634, including the circRNA expression profiles in patients with PDAC were screened and downloaded from the GEO database. The GSE69362 dataset included circRNA expression profiles from six PDAC tissues and six paired adjacent normal tissues (24). The GSE79634 dataset included circRNA expression profiles from 20 PDAC tissues and 20 paired adjacent normal tissues (23).
The GEOquery (http://www.bioconductor.org/packages/release/bioc/html/GEOquery.html) and limma (http://www.bioconductor.org/packages/release/bioc/html/limma.html) packages in R software (version 3.3.2; http://www.r-project.org) were used to process the expression matrix and differential expression analysis, as previously described (25). Principal component analysis (PCA) was performed using the Factoextra package in R software (version 1.0.7; http://cran.r-project.org/web/packages/factoextra/index.html). Log2 [fold change (FC)]≥|1| and an adjusted P-value (Padj)≤0.05 were set as thresholds to identify the DEcircRNAs, which were presented as volcano plots using the ggplot2 package (version 3.3.5; http://cran.r-project.org/web/packages/ggplot2/index.html) in R software (version 3.3.2; http://www.r-project.org).
Gene Ontology (GO; http://geneontology.org) and Kyoto Encyclopedia of Genes and Genomes (KEGG; http://www.kegg.jp) enrichment analyses were performed to assess the DEcircRNAs, based on the identified genes. The overlapping upregulated and downregulated DEcircRNAs in both GSE69362 and GSE79634 datasets were displayed using Venn diagrams. A total of nine DEcircRNAs were identified. The circRNA-miRNA network, depicting the interactions between the nine candidate circRNAs and their target miRNAs, was predicted using Miranda software (version 3.3a; http://anaconda.org/bioconda/miranda).
Patients and samples
To further validate the correlation between the expression profiles of the nine candidate circRNAs and the clinicopathological characteristics of patients with PDAC, PDAC and paired adjacent normal tissues were collected from 60 patients with PDAC who underwent surgery between November 2016 and December 2019. Para-cancer tissues were taken 2 cm away from the tumor margin. There were 36 male patients and 24 female patients. The mean age of patients with PDAC was 63.3±7.2 years (range, 43–75 years). The surgery for patients with PDAC was performed at Union Hospital, Tongji Medical College, Huazhong University of Science and Technology (Wuhan, China). The inclusion criteria for all patients with PDAC were: i) Pathologically confirmed as PDAC; ii) age >18 years; iii) underwent surgical resection; iv) had snap-frozen PDAC and paired adjacent normal tissues; and v) without neoadjuvant therapy. The exclusion criteria were: i) Incomplete clinical data and survival data; and ii) complicated with other malignancies. The PDAC and paired adjacent normal tissues were snap-frozen in liquid nitrogen immediately after surgery. The specimens were stored at −80°C. All patients were followed up every 3–6 months by clinic visits or telephone calls. The median follow-up duration was 16.0 months (range, 2.0-36.0 months; between November 2016 and December 2019), and the last follow-up date was December 31, 2019. The present study was approved by the Ethics Committee of Tongji Medical College, Huazhong University of Science and Technology [approval no. (2014), ethics approval no. (S108)]. Written informed consent was provided by all patients or their legal representatives prior to the study start.
Clinical data collection
The clinical data of patients were reviewed, and the clinical characteristics [age, sex, smoking and drinking history, tumor location, pathological grade, tumor size, lymph node metastasis (LNM), T stage, N stage and TNM stage] were recorded. Tumor staging was performed according to the American Joint Committee on Cancer (version 8) TNM staging system (26). Survival data were extracted from the patients' follow-up documents, which were used to measure overall survival (OS) time. Follow-up lasted from November 2016 to December 2019. Patients were followed up every 3–6 months by clinic visits or telephone calls.
Determination of candidate circRNAs
The expression levels of the nine candidate circRNAs were detected in 60 PDAC tissues and paired adjacent normal tissues via RT-qPCR analysis. Total RNA was extracted from PDAC tissues and normal tissues using TRIzol® reagent (Thermo Fisher Scientific, Inc.). The linear RNA was subsequently digested using RNase R (Epicentre; Illumina, Inc.) and reverse transcribed into cDNA using the PrimeScript® RT reagent kit (Takara Biotechnology Co., Ltd.). qPCR was subsequently performed using TB Green® Fast qPCR Mix (Takara Biotechnology Co., Ltd.). The primer sequences used for qPCR are listed in Table SI. The specific conditions for RT were as follows: 37°C for 15 min and 85°C for 5 sec. The thermocycling conditions for qPCR were as follows: Initial denaturation at 95°C for 30 sec, followed by 40 cycles of 95°C for 5 sec and 61°C for 10 sec. The relative expression was calculated using the 2−ΔΔCq method (27).
Classification of candidate circRNAs
The candidate circRNAs were classified into high- and low-expression groups according to their median value in the tumor tissue, as follows: 3.345 for circ_0000515, 2.564 for circ_0000517, 2.114 for circ_0000520, 2.999 for circ_0000514, 4.675 for circ_0011385, 1.865 for circ_0055033, 2.395 for circ_0072088, 1.594 for circ_0003528, 1.086 for circ_0008514.
Statistical analysis
Data processing and graphs were plotted using R software (version 3.3.2; http://www.r-project.org), SPSS 22.0 software (IBM Corp.) or GraphPad Prism 7.02 software (GraphPad Software Inc.). Data are presented as the mean ± SD for continuous data with normal distribution, median with interquartile range for continuous data with skewed distribution and number with percentage for categorical data. Data distribution was determined using the Kolmogorov-Smirnov test. The Wilcoxon signed-rank sum test was used to compare differences in the expression levels of the candidate circRNAs between PDAC tissues and adjacent normal tissues. Receiver operating characteristic (ROC) curve analysis was performed to analyze the performance of the candidate circRNAs in distinguishing between PDAC tissues and adjacent normal tissues. Spearman's rank correlation analysis was performed to assess the correlation between the circRNAs and the clinicopathological characteristics of patients with PDAC. The OS time was measured from the date of surgery to mortality or the last follow-up via the Kaplan-Meier method and log-rank test. P<0.05 was considered to indicate a statistically significant difference.
Results
circRNA expression profiles and enrichment analysis
In the GSE79634 dataset, PCA analysis revealed that circRNAs were differentially expressed between 20 PDAC tissues and 20 paired adjacent normal tissues (Fig. 1A). The volcano plot revealed that there were 161 upregulated and 128 downregulated DEcircRNAs in PDAC tissues compared with paired adjacent normal tissues (Fig. 1B). Furthermore, GO enrichment analysis demonstrated that the DEcircRNAs were predominantly enriched in the ‘ATP binding’ in terms of molecular function, the ‘cytosol’ in terms of cellular component and the ‘G-protein coupled receptor signaling pathway’ in terms of biological function (Fig. 1C). In addition, the most significantly enriched pathways in the KEGG enrichment analysis associated with the candidate DEcircRNAs were the ‘regulation of actin cytoskeleton’, ‘protein processing in endoplasmic reticulum’, ‘cGMP-PKG signaling’ and ‘insulin signaling’ pathways (Fig. 1D). These results suggested that the circRNAs in the GSE79634 dataset were differently expressed between the tumor tissue and adjacent tissue with 161 upregulated and 128 downregulated DEcircRNAs, and they may be involved in several cellular process.
In the GSE69362 dataset, PCA analysis revealed that circRNAs were differentially expressed between six PDAC tissues and six paired adjacent normal tissues (Fig. 2A). The volcano plot revealed that there were 64 upregulated and 10 downregulated DEcircRNAs in PDAC tissues compared with normal tissues (Fig. 2B). Furthermore, GO enrichment analysis demonstrated that the DEcircRNAs were predominantly enriched in the ‘zinc ion binding’ in terms of molecular function, the ‘cytoplasm’ in terms of cellular component and the ‘mitotic nuclear envelope disassembly’ in terms of biological process (Fig. 2C). In addition, the most significantly enriched pathways in the KEGG enrichment analysis associated with the candidate DEcircRNAs were the ‘RNA transport’, ‘prostate cancer’, ‘melanogenesis’ and ‘insulin resistance’ signaling pathways (Fig. 2D). These results suggested that the circRNAs in the GSE69362 dataset were differently expressed between the tumor tissue and adjacent tissue with 64 upregulated and 10 downregulated DEcircRNAs, and they might be involved in several cellular process.
Selection of candidate circRNAs
Further analysis revealed that 22 DEcircRNAs were overlapped between the GSE79634 and GSE69362 datasets (Fig. 3A). Among the overlapping DEcircRNAs, nine were upregulated in PDAC tissues compared with paired adjacent normal tissues in both datasets (Fig. 3B). These nine DEcircRNAs were selected as candidate circRNAs and their target miRNAs were predicted. The regulatory network of the DEcircRNAs and their target miRNAs is presented in Fig. 4. The information of these nine candidate circRNAs is presented in Table I, which includes the probe name, located chromosome, start, end, type, gene symbol, expression trend, log2(FC), P-value and Padj-value in the GSE79634 and GSE69362 datasets. These results suggested that 9 upregulated in tumor tissue, overlapping DEcircRNAs between GSE79634 and GSE69362 datasets were identified as candidate circRNAs.
Table I.Information on the nine candidate circRNAs screened from the GSE79634 and GSE69362 datasets. |
Characteristics of patients with PDAC
Among the 60 patients with PDAC, 36 (60.0%) were men and 24 (40.0%) were women, with a mean age of 63.3±7.2 years, and the number of patients with tumor location at pancreas head, pancreas body and pancreas tail was 27 (45.0%), 21 (35.0%) and 12 (20.0%), respectively. The number of patients with G1, G2 and G3 pathological grade was 8 (13.3%), 26 (43.3%) and 26 (43.3%), respectively. The mean tumor size was 3.8±1.5 cm. A total of 42 patients (70.0%) had PDAC LNM. In addition, the number of patients with IA, IB, IIA, IIB and III TNM stage was 4 (6.7%), 4 (6.7%), 8 (13.3%), 28 (46.7%) and 16 (26.7%), respectively. All clinicopathological characteristics are presented in Table II. These results indicated the characteristics of patients with PDAC enrolled in the present study.
Candidate circRNA expression levels in patients with PDAC
Among the nine candidate circRNAs, the expression levels of circ_0000515 (P<0.001; Fig. 5A), circ_0000517 (P<0.001; Fig. 5B), circ_0000520 (P<0.001; Fig. 5C), circ-0000514 (P<0.001; Fig. 5D), circ_0011385 (P<0.001; Fig. 5E), circ_0055033 (P<0.001; Fig. 5F), circ_0072088 (P<0.001; Fig. 5G) and circ_0003528 (P<0.001; Fig. 5H) were significantly upregulated in PDAC tissues compared with paired adjacent normal tissues. However, circ_0008514 expression (P=0.155; Fig. 5I) was not significantly different between PDAC tissues and paired adjacent normal tissues. Furthermore, ROC curve analysis demonstrated that circ_0000515 (Fig. 6A), circ_0000517 (Fig. 6B), circ_0000520 (Fig. 6C), circ_0000514 (Fig. 6D), circ_0011385 (Fig. 6E) and circ_0072088 (Fig. 6G) exhibited good area under the curve (AUC) values to distinguish PDAC tissues from paired adjacent normal tissues. The AUC values for these circRNAs were 0.920, 0.922, 0.887, 0.899, 0.953 and 0.902, respectively. Conversely, circ-0055033 (Fig. 6F) and circ_0003528 (Fig. 6H) exhibited ordinary values in differentiating PDAC tissues from paired adjacent normal tissues, with AUC values of 0.697 and 0.727, respectively. Notably, circ_0008514 (Fig. 6I) failed to distinguish between PDAC tissues and paired adjacent normal tissues. These findings suggested that eight candidate circRNAs were highly expressed in PDAC tissues compared with paired adjacent normal tissues and they could distinguish PDAC tissues from paired adjacent normal tissues.
Correlation between the expression levels of the candidate circRNAs and the clinicopathological characteristics of patients with PDAC
Spearman's rank correlation analysis was performed to assess the correlation between the circRNAs and the clinicopathological characteristics of patients with PDAC. The results demonstrated that tumor circ_0000515 expression was positively correlated with T stage (P=0.038) and TNM stage (P=0.013) in patients with PDAC. In addition, tumor circ_0000514 expression was positively correlated with T stage (P=0.024), while tumor circ_0011385 expression was positively correlated with T stage (P=0.024), N stage (P=0.010) and TNM stage (P<0.001). Furthermore, tumor circ_0072088 expression was positively correlated with T stage (P=0.021) and TNM stage (P=0.011) in patients with PDAC (Table III). The aforementioned findings mentioned demonstrated that four candidate circRNAs were associated with aggravating clinicopathological characteristics of patients with PDAC.
Table III.Correlation between tumor circRNA expression levels and the clinicopathological characteristics of patients with pancreatic ductal adenocarcinoma. |
Association between the expression levels of the candidate circRNAs and OS time in patients with PDAC
The OS time was significantly shorter in patients with high circ_0000515 expression than those with low circ_0000515 expression (P=0.013; Fig. 7A). Furthermore, OS time was significantly shorter in patients with high circ_0011385 expression than those with low circ_0011385 expression (P=0.014; Fig. 7E). However, the expression levels of circ_0000517 (P=0.128; Fig. 7B), circ_0000520 (P=0.163; Fig. 7C), circ_0000514 (P=0.135; Fig. 7D), circ_0055033 (P=0.184; Fig. 7F), circ_0072088 (P=0.271; Fig. 7G), circ_0003528 (P=0.667; Fig. 7H) and circ_0008514 (P=0.862; Fig. 7I) in PDAC tissues were not associated with OS time. As aforementioned, high levels of these two candidate circRNAs (circ_0000515 high and circ_0011385 high) were associated with shorter OS times.
RT-qPCR analysis demonstrated that among the nine candidate circRNAs in patients with PDAC, eight candidate circRNAs were markedly dysregulated in PDAC tissues compared with paired adjacent normal tissues; four were correlated with tumor stages and two were associated with OS in patients with PDAC (Table IV). Notably, the expression levels of circ_0000515 and circ_0011385 were upregulated in PDAC tissues compared with paired adjacent normal tissues, and were correlated with tumor stage, and associated with OS of patients with PDAC. Thus, these two candidate circRNAs may be used as potential biomarkers in the treatment of PDAC.
Discussion
The present study performed a secondary analysis based on datasets from two previous studies, namely GSE79634 and GSE69362, and candidate circRNAs were identified among the DEcircRNAs (22,28). The expression levels of the candidate circRNAs and their correlation with the clinicopathological characteristics of patients with PDAC were evaluated in a larger sample size. It was revealed that nine circRNAs exhibited the same expression profile in both datasets. These circRNAs were selected as candidate circRNAs and their expression profile in tissue samples from 60 patients with PDAC was assessed via RT-qPCR analysis. The results demonstrated that 8/9 candidate circRNAs exhibited differentiated expression profiles in tumor tissues compared with paired adjacent normal tissues. Furthermore, five candidate circRNAs were positively correlated with tumor stage, and two (circ_0000515 and circ_0011385) were negatively associated with OS in patients with PDAC. Notably, circ_0000515 and circ_0011385 were not only highly expressed in PDAC tissues compared with normal tissues, but were also correlated with more advanced TNM stage and shorter survival time in patients with PDAC.
The roles of circRNAs in PDAC remain unclear, unlike in other solid tumors, such as gastric carcinoma and nasopharyngeal carcinoma (29,30). A previous study reported that circ-ASH2-like histone lysine methyltransferase complex subunit (ASH2L) is upregulated in PDAC cells, which enhances PDAC cell proliferation, migration and invasion capacities (31). Another study demonstrated that circ-bifunctional apoptosis regulator (BFAR) elevates the proliferation, migration and invasion of PDAC cells, and promotes the growth and metastasis of tumors in PDAC animal models (32). Furthermore, circ-BFAR can positively regulate the expression levels of epithelial-to-mesenchymal transition markers by mediating the miR-34b-5p/mesenchymal-to-epithelial transition factor/protein kinase B (Akt) axis in PDAC cells (32). Only a few studies have investigated the correlation between the expression of circRNAs and the clinicopathological characteristics of patients with PDAC (33,34). However, a previous study reported that decreased circ_0001649 expression in PDAC tissues is associated with low tumor stage and increased differentiation level in patients (35). Taken together, these studies suggest that certain circRNAs are associated with disease progression and may serve as potential biomarkers in PDAC.
The present study identified nine circRNAs presenting with the same expression trends in the two datasets (GSE79634 and GSE69362), which were selected as candidate circRNAs for RT-qPCR analyses in a cohort of 60 patients with PDAC. The results demonstrated that 8/9 candidate circRNAs were notably upregulated in PDAC tissues compared with paired adjacent normal tissues, and five of them were positively associated with TNM stage in patients with PDAC. This may be due to that these circRNAs may promote the malignant behaviors of PDAC cells by interacting with other tumor-related factors or signaling pathways, which in turn can promote tumor progression, eventually leading to a more advanced tumor stage in patients with PDAC. For instance, as reported by a previous study about other circRNAs in PDAC, these circRNAs might sponge miRNAs to enhance PDAC cell proliferation, invasion and migration (32). However, regarding the five candidate circRNAs identified in the present study, no mechanistic studies have been reported to date; therefore, in vivo and in vitro studies are urgently required to verify their effects on PDAC.
In the present study, two candidate circRNAs, circ_0000515 and circ_0011385, were not only positively associated with TNM stage, but also negatively associated with OS time in patients with PDAC. Thus, it was hypothesized that these two candidate circRNAs may serve as prognostic factors in PDAC management. To the best of our knowledge, the present study was the first to report the potential role of these two circRNAs as biomarkers in PDAC. Similarly, other circRNAs, including circ_0030235 and circ_0007534, have been identified as prognostic biomarkers of PDAC (33–36). A previous study suggested that circ-ASH2L may serve as a prognostic biomarker in patients with PDAC as its high expression in PDAC tissues was associated with poor survival outcomes (31). Another study reported that circ_0001649 expression is downregulated in PDAC tissues compared with normal tissues, and multivariate regression analysis revealed that its expression may be an independent prognostic factor for survival (37). A previous study demonstrated that circ_0030235 expression is upregulated in PDAC tissues, and its expression is associated with poor prognosis (35). In terms of the circRNAs identified in the present study, circ_0000515 and circ_0011385 have not yet been identified in PDAC. However, they have been reported in different types of cancer. For example, an in vivo and in vitro study revealed that circ_0000515 enhances progression of cervical cancer by sponging miR-326 expression via increasing E-twenty six transcription factor ELK1 expression (38). Another study reported that circ_0011385 promotes thyroid cancer development by sponging miR-361-3p expression in vitro and in vivo (39). The results of the present study may be explained as follows: Several target miRNAs of these two candidate circRNAs function as antitumor factors in different types of cancer. For example, the target miRNA of circ_0000515, miR-939, and the target miRNA of circ_0011385, miR-615, are both considered tumor suppressors that inhibit cancer cell invasion and migration in various types of cancer (40–43). Thus, it was hypothesized that circ_0000515 and circ_0011385 may potentially function as regulators in enhancing the malignant behavior of PDAC by sponging their target miRNAs, which promotes the tumor progression of PDAC. In addition, circ_0000515 and circ_0011385 may serve as biomarkers for PDAC management in clinical practice.
The present study is not without limitations. First, the statistical power can be diminished due to the relatively small sample size of 60 patients with PDAC. Secondly, the molecular functions of the candidate circRNAs in PDAC were not investigated in the present study, which should be validated by in vitro and in vivo experiments. Thirdly, microarray analysis may present some bias (44); however, the present study performed RT-qPCR analysis to validate the expression trends of the candidate circRNAs in patients with PDAC. Furthermore, a control group of healthy individuals was not included in the present study. However, it was difficult to acquire pancreatic tissue samples from healthy individuals. Thus, prospective studies will aim to include this control cohort. Fourthly, the expression levels of the circRNAs were only assessed in tissue samples but not peripheral samples, which is inconvenient for clinical application. This was the case as circRNAs are abundantly expressed in tissue samples compared with peripheral blood (19,45).
In conclusion, the results of the present study provide evidence that circ_0000515 and circ_0011385 may serve as biomarkers for disease control and determine the prognosis of patients with PDAC, which implies their potential for guiding the management of patients with PDAC.
Supplementary Material
Supporting Data
Acknowledgements
Not applicable.
Funding
Funding: No funding was received.
Availability of data and materials
The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.
Authors' contributions
YX conceived, designed and supervised the present study, and revised the manuscript for important intellectual content. HW performed the experiments and provided technical support. BW and LW analyzed the data and drafted the initial manuscript. YX and HW confirm the authenticity of all the raw data. All authors have read and approved the final manuscript.
Ethics approval and consent to participate
The present study was approved by the Ethics Committee of Tongji Medical College, Huazhong University of Science and Technology [approval number (2014), ethics approval no. (S108)]. Written informed consent was provided by all patients or their legal representatives prior to the study commencement.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
Glossary
Abbreviations
Abbreviations:
PDAC |
pancreatic ductal adenocarcinoma |
circRNA |
circular RNA |
DEcircRNAs |
differentially expressed circRNAs |
GO |
Gene Ontology |
KEGG |
Kyoto Encyclopedia of Genes and Genomes |
LNM |
lymph node metastasis |
OS |
overall survival |
ROC |
receiver operating characteristic |
BFAR |
bifunctional apoptosis regulator |
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