TCF12 activates MAGT1 expression to regulate the malignant progression of pancreatic carcinoma cells

Wang,Ling; Tang,Yanjiao; Wu,Hongyi; Shan,Guiqiu

doi:10.3892/ol.2021.13180

TCF12 activates MAGT1 expression to regulate the malignant progression of pancreatic carcinoma cells

Authors:
- Ling Wang
- Yanjiao Tang
- Hongyi Wu
- Guiqiu Shan
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Affiliations: Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan, Shandong 250014, P.R. China, Medical Laboratory, Shenzhen Sami Medical Center, Shenzhen, Guangdong 518038, P.R. China, Medical Laboratory, Huizhou Municipal Central Hospital, Huizhou, Guangdong 516008, P.R. China, Department of Transfusion Medicine, General Hospital of Southern Theater Command, Guangzhou, Guangdong 510010, P.R. China
Published online on: December 27, 2021 https://doi.org/10.3892/ol.2021.13180
Article Number: 62
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

As a highly malignant gastrointestinal tumor, pancreatic carcinoma (PC) has poor prognosis due to its low early diagnosis rate, advanced tumor resection and chemotherapy resistance. Magnesium transporter 1 (MAGT1) is a magnesium ion transporter located on the cell membrane, which shows promotive effects on biological behaviors of multiple tumor cells. The aim of the present study was to investigate the role of MAGT1 in the progression of PC and its potential molecular mechanism. Based on the Gene Expression Profiling Interactive Analysis website, MAGT1 was highly expressed in tissues from patients with PC and was associated with poor prognosis. In functional experiments, MAGT1 was highly expressed in PC cell lines. The Cell Counting Kit‑8, gap closure and Transwell assays, and western blot analysis, were used to investigate the effects of MAGT1 overexpression or knockdown on the biological behaviors of PC cells. It was found that MAGT1 promoted the proliferation, migration and invasion of PC cells in vitro. According to the Encyclopedia of RNA Interactomes website, transcription factor 12 (TCF12) mRNA expression level was positively correlated with MAGT1 expression level in the tissues from patients with PC. Positive targeting regulation of MAGT1 by TCF12 was also confirmed using a dual‑luciferase gene reporter assay and chromatin immunoprecipitation. In addition, knockdown of TCF12 expression inhibited the proliferation and migration of PC cells, but overexpression of MAGT1 expression partly reversed this. These results suggested that TCF12 could promote the proliferation, migration and invasion of PC cells by activating MAGT1 expression, which was associated with poor prognosis. These findings suggest that MAGT1 could be a promising biomarker for the occurrence, progression and prognosis of PC.

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