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PKMYT1 regulates the proliferation and epithelial‑mesenchymal transition of oral squamous cell carcinoma cells by targeting CCNA2

  • Authors:
    • Ye Cai
    • Weidong Yang
  • View Affiliations / Copyright

    Affiliations: Department of Endodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, Jiangsu 210008, P.R. China
    Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 63
    |
    Published online on: December 27, 2021
       https://doi.org/10.3892/ol.2021.13181
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Abstract

Oral squamous cell carcinoma (OSCC) has gradually become a global public health issue in recent years. Therefore, the current study aimed to explore the mechanism of OSCC development and to identify a potential target that may be used in its treatment. The expression of protein kinase, membrane‑associated tyrosine/threonine 1 (PKMYT1) and cyclin A2 (CCNA2) in SCC‑9 cells was determined prior to and following transfection with short hairpin RNA targeting PKMYT1. Cell proliferation, colony‑forming ability, migration and invasion were determined using Cell Counting Kit‑8, colony formation, wound healing and Transwell assays, respectively. Furthermore, the expression of epithelial‑mesenchymal transition (EMT)‑ and migration‑related proteins were evaluated using western blot analysis. Additionally, co‑immunoprecipitation was used to verify the binding of PKMYT1 and CCNA2. The results revealed that PKMYT1 was highly expressed in OSCC cells and that PKMYT1 knockdown could inhibit proliferation, colony formation, migration, invasion, EMT and CCNA2 expression in SCC‑9 cells. In addition, PKMYT1 was demonstrated to bind to CCNA2, and knocking down PKMYT1 resulted in inhibitory effects on cell proliferation, colony formation ability, migration, invasion and EMT by downregulating CCNA2 expression. PKMYT1 was observed to regulate the proliferation, migration and EMT of OSCC cells by targeting CCNA2, which may be used in the future to improve OSCC treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Cai Y and Yang W: PKMYT1 regulates the proliferation and epithelial‑mesenchymal transition of oral squamous cell carcinoma cells by targeting CCNA2. Oncol Lett 23: 63, 2022.
APA
Cai, Y., & Yang, W. (2022). PKMYT1 regulates the proliferation and epithelial‑mesenchymal transition of oral squamous cell carcinoma cells by targeting CCNA2. Oncology Letters, 23, 63. https://doi.org/10.3892/ol.2021.13181
MLA
Cai, Y., Yang, W."PKMYT1 regulates the proliferation and epithelial‑mesenchymal transition of oral squamous cell carcinoma cells by targeting CCNA2". Oncology Letters 23.2 (2022): 63.
Chicago
Cai, Y., Yang, W."PKMYT1 regulates the proliferation and epithelial‑mesenchymal transition of oral squamous cell carcinoma cells by targeting CCNA2". Oncology Letters 23, no. 2 (2022): 63. https://doi.org/10.3892/ol.2021.13181
Copy and paste a formatted citation
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Spandidos Publications style
Cai Y and Yang W: PKMYT1 regulates the proliferation and epithelial‑mesenchymal transition of oral squamous cell carcinoma cells by targeting CCNA2. Oncol Lett 23: 63, 2022.
APA
Cai, Y., & Yang, W. (2022). PKMYT1 regulates the proliferation and epithelial‑mesenchymal transition of oral squamous cell carcinoma cells by targeting CCNA2. Oncology Letters, 23, 63. https://doi.org/10.3892/ol.2021.13181
MLA
Cai, Y., Yang, W."PKMYT1 regulates the proliferation and epithelial‑mesenchymal transition of oral squamous cell carcinoma cells by targeting CCNA2". Oncology Letters 23.2 (2022): 63.
Chicago
Cai, Y., Yang, W."PKMYT1 regulates the proliferation and epithelial‑mesenchymal transition of oral squamous cell carcinoma cells by targeting CCNA2". Oncology Letters 23, no. 2 (2022): 63. https://doi.org/10.3892/ol.2021.13181
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