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Tetramethylpyrazine inhibits the proliferation, invasiveness and migration of cervical cancer C33A cells by retarding the Hedgehog signaling pathway

  • Authors:
    • Jing Ren
    • Jiping Cai
    • Changfeng Wang
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry, Shijiazhuang Medical College, Shijiazhuang, Hebei 050000, P.R. China
    Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 66
    |
    Published online on: January 3, 2022
       https://doi.org/10.3892/ol.2022.13185
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Abstract

Cervical carcinoma (CC) ranks among the top four most common cancers in women worldwide. Over the last 10 years, several studies have confirmed the inhibitory effects of tetramethylpyrazine (TMP) on numerous types of cancer. To investigate the inhibitory effect of TMP on the CC C33A cell line, MTT and colony formation assays were performed to determine how TMP affects C33A cell survival and proliferation. Proliferation‑, migration‑ and hedgehog (Hh) signaling pathway‑related protein expression levels were analyzed via western blotting. Wound‑healing and Transwell assays were used to detect the migration and invasion abilities of C33A cells, respectively. The results indicated that TMP markedly reduced the C33A cell survival rate compared with the cervical epithelial Ect1 cell line, which was unaffected by TMP treatment. C33A cell proliferation was downregulated by TMP treatment in a dose‑dependent manner. TMP treatment also significantly inhibited C33A cell migration and invasiveness in a dose‑dependent manner. Furthermore, TMP inhibited the Hh signaling pathway, as demonstrated by a dose‑dependent reduction in Hh‑related protein expression levels following TMP treatment. Subsequently, treatment with smoothened agonist increased the proliferation, invasiveness and migration abilities of TMP‑treated C33A cells. In conclusion, TMP inhibited the proliferation, migration and invasiveness of CC cells via inhibition of the Hh signaling pathway.
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View References

1 

Vu M, Yu J, Awolude OA and Chuang L: Cervical cancer worldwide. Curr Probl Cancer. 42:457–465. 2018. View Article : Google Scholar : PubMed/NCBI

2 

Ou R, Lv J, Zhang Q, Lin F, Zhu L, Huang F, Li X, Li T, Zhao L, Ren Y and Xu Y: circAMOTL1 Motivates AMOTL1 expression to facilitate cervical cancer growth. Mol Ther Nucleic Acids. 19:50–60. 2020. View Article : Google Scholar : PubMed/NCBI

3 

Ki EY, Lee KH, Park JS and Hur SY: A clinicopathological review of pulmonary metastasis from uterine cervical cancer. Cancer Res Treat. 48:266–272. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Hwang JH, Yoo HJ, Lim MC, Seo SS, Kang S, Kim JY and Park SY: Brain metastasis in patients with uterine cervical cancer. J Obstet Gynaecol Res. 39:287–291. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Hong JH, Tsai CS, Lai CH, Chang TC, Wang CC, Chou HH, Lee SP and Hsueh S: Recurrent squamous cell carcinoma of cervix after definitive radiotherapy. Int J Radiat Oncol Biol Phys. 60:249–257. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Li H, Wu X and Cheng X: Advances in diagnosis and treatment of metastatic cervical cancer. J Gynecol Oncol. 27:e432016. View Article : Google Scholar : PubMed/NCBI

7 

Wendel Naumann R and Leath CA III: Advances in immunotherapy for cervical cancer. Curr Opin Oncol. 32:481–487. 2020. View Article : Google Scholar : PubMed/NCBI

8 

Yang A, Farmer E, Wu TC and Hung CF: Perspectives for therapeutic HPV vaccine development. J Biomed Sci. 23:752016. View Article : Google Scholar : PubMed/NCBI

9 

Lopez MS, Baker ES, Maza M, Fontes-Cintra G, Lopez A, Carvajal JM, Nozar F, Fiol V and Schmeler KM: Cervical cancer prevention and treatment in Latin America. J Surg Oncol. 115:615–618. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Zhao Y, Liu Y and Chen K: Mechanisms and clinical application of tetramethylpyrazine (an interesting natural compound isolated from Ligusticum Wallichii): Current status and perspective. Oxid Med Cell Longev. 2016:21246382016. View Article : Google Scholar : PubMed/NCBI

11 

Shi J, Li R, Yang S, Phang Y, Zheng C and Zhang H: The protective effects and potential mechanisms of Ligusticum chuanxiong: Focus on anti-inflammatory, antioxidant, and antiapoptotic activities. Evid Based Complement Alternat Med. 2020:82059832020. View Article : Google Scholar : PubMed/NCBI

12 

Yuan Z, Zhang J and Yang C: Ligusticum wallichii extract inhibited the expression of IL-1β after AMI in Rats. Evid Based Complement Alternat Med. 2014:6203592014. View Article : Google Scholar : PubMed/NCBI

13 

Zhao Z and Moghadasian MH: Chemistry, natural sources, dietary intake and pharmacokinetic properties of ferulic acid: A review. Food Chem. 109:691–702. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Wu B, Liu M, Liu H, Li W, Tan S, Zhang S and Fang Y: Meta-analysis of traditional Chinese patent medicine for ischemic stroke. Stroke. 38:1973–1979. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Wang P, She G, Yang Y, Li Q, Zhang H, Liu J, Cao Y, Xu X and Lei H: Synthesis and biological evaluation of new ligustrazine derivatives as anti-tumor agents. Molecules. 17:4972–4985. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Lin LN, Wang WT and Xu ZJ: Clinical study on ligustrazine in treating myocardial ischemia and reperfusion injury. Zhongguo Zhong Xi Yi Jie He Za Zhi. 17:261–263. 1997.(In Chinese). PubMed/NCBI

17 

Zhou Y, Ji Z, Yan W, Zhou Z, Li H and Xiao Y: Tetramethylpyrazine inhibits prostate cancer progression by downregulation of forkhead box M1. Oncol Rep. 38:837–842. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Huang HH, Liu FB, Ruan Z, Zheng J, Su YJ and Wang J: Tetramethylpyrazine (TMPZ) triggers S-phase arrest and mitochondria-dependent apoptosis in lung cancer cells. Neoplasma. 65:367–375. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Wang S, Lei T and Zhang M: The reversal effect and its mechanisms of tetramethylpyrazine on multidrug resistance in human bladder cancer. PLoS One. 11:e01577592016. View Article : Google Scholar : PubMed/NCBI

20 

Zhou Y, Zhou Z, Ji Z, Yan W, Li H and Yu X: Tetramethylpyrazine reduces prostate cancer malignancy through inactivation of the DPP10AS1/CBP/FOXM1 signaling pathway. Int J Oncol. 57:314–324. 2020.PubMed/NCBI

21 

Shen J, Zeng L, Pan L, Yuan S, Wu M and Kong X: Tetramethylpyrazine regulates breast cancer cell viability, migration, invasion and apoptosis by affecting the activity of Akt and caspase-3. Oncol Lett. 15:4557–4563. 2018.PubMed/NCBI

22 

Skoda AM, Simovic D, Karin V, Kardum V, Vranic S and Serman L: The role of the Hedgehog signaling pathway in cancer: A comprehensive review. Bosn J Basic Med Sci. 18:8–20. 2018. View Article : Google Scholar : PubMed/NCBI

23 

Wu F, Zhang Y, Sun B, McMahon AP and Wang Y: Hedgehog signaling: From basic biology to cancer therapy. Cell Chem Biol. 24:252–280. 2017. View Article : Google Scholar : PubMed/NCBI

24 

Hu J, Cao G, Wu X, Cai H and Cai B: Tetramethylpyrazine inhibits activation of hepatic stellate cells through hedgehog signaling pathways in vitro. Biomed Res Int. 2015:6030672015. View Article : Google Scholar : PubMed/NCBI

25 

Huang C, Lu H, Li J, Xie X, Fan L, Wang D, Tan W, Wang Y, Lin Z and Yao T: SOX2 regulates radioresistance in cervical cancer via the hedgehog signaling pathway. Gynecol Oncol. 151:533–541. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Luan Y, Liu J, Liu X, Xue X, Kong F, Sun C, Wang J, Liu L and Jia H: Tetramethypyrazine inhibits renal cell carcinoma cells through inhibition of NKG2D signaling pathways. Int J Oncol. 49:1704–1712. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Feng J, Wang C, Liu T, Li J, Wu L, Yu Q, Li S, Zhou Y, Zhang J, Chen J, et al: Procyanidin B2 inhibits the activation of hepatic stellate cells and angiogenesis via the Hedgehog pathway during liver fibrosis. J Cell Mol Med. 23:6479–6493. 2019. View Article : Google Scholar : PubMed/NCBI

28 

Shen X, Li L, He Y, Lv X and Ma J: Raddeanin A inhibits proliferation, invasion, migration and promotes apoptosis of cervical cancer cells via regulating miR-224-3p/Slit2/Robo1 signaling pathway. Aging. 13:7166–7179. 2021. View Article : Google Scholar : PubMed/NCBI

29 

de Sanjosé S, Brotons M and Pavón MA: The natural history of human papillomavirus infection. Best Pract Res Clin Obstet Gynaecol. 47:2–13. 2018. View Article : Google Scholar : PubMed/NCBI

30 

Menderes G, Black J, Schwab CL and Santin AD: Immunotherapy and targeted therapy for cervical cancer: An update. Expert Rev Anticancer Ther. 16:83–98. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Kaliff M, Karlsson MG, Sorbe B, Bohr Mordhorst L, Helenius G and Lillsunde-Larsson G: HPV-negative tumors in a swedish cohort of cervical cancer. Int J Gynecol Pathol. 39:279–288. 2020. View Article : Google Scholar : PubMed/NCBI

32 

Zhao S, Zhang Z, Yao Z, Shao J, Chen A, Zhang F and Zheng S: Tetramethylpyrazine attenuates sinusoidal angiogenesis via inhibition of hedgehog signaling in liver fibrosis. IUBMB Life. 69:115–127. 2017. View Article : Google Scholar : PubMed/NCBI

33 

Wu Z, Zou B, Zhang X and Peng X: Eupatilin regulates proliferation and cell cycle of cervical cancer by regulating hedgehog signalling pathway. Cell Biochem Funct. 38:428–435. 2020. View Article : Google Scholar : PubMed/NCBI

34 

Zhang F, Ren CC, Liu L, Chen YN, Yang L, Zhang XA, Wang XM and Yu FJ: SHH gene silencing suppresses epithelial-mesenchymal transition, proliferation, invasion, and migration of cervical cancer cells by repressing the hedgehog signaling pathway. J Cell Biochem. 119:3829–3842. 2018. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ren J, Cai J and Wang C: Tetramethylpyrazine inhibits the proliferation, invasiveness and migration of cervical cancer C33A cells by retarding the Hedgehog signaling pathway. Oncol Lett 23: 66, 2022.
APA
Ren, J., Cai, J., & Wang, C. (2022). Tetramethylpyrazine inhibits the proliferation, invasiveness and migration of cervical cancer C33A cells by retarding the Hedgehog signaling pathway. Oncology Letters, 23, 66. https://doi.org/10.3892/ol.2022.13185
MLA
Ren, J., Cai, J., Wang, C."Tetramethylpyrazine inhibits the proliferation, invasiveness and migration of cervical cancer C33A cells by retarding the Hedgehog signaling pathway". Oncology Letters 23.2 (2022): 66.
Chicago
Ren, J., Cai, J., Wang, C."Tetramethylpyrazine inhibits the proliferation, invasiveness and migration of cervical cancer C33A cells by retarding the Hedgehog signaling pathway". Oncology Letters 23, no. 2 (2022): 66. https://doi.org/10.3892/ol.2022.13185
Copy and paste a formatted citation
x
Spandidos Publications style
Ren J, Cai J and Wang C: Tetramethylpyrazine inhibits the proliferation, invasiveness and migration of cervical cancer C33A cells by retarding the Hedgehog signaling pathway. Oncol Lett 23: 66, 2022.
APA
Ren, J., Cai, J., & Wang, C. (2022). Tetramethylpyrazine inhibits the proliferation, invasiveness and migration of cervical cancer C33A cells by retarding the Hedgehog signaling pathway. Oncology Letters, 23, 66. https://doi.org/10.3892/ol.2022.13185
MLA
Ren, J., Cai, J., Wang, C."Tetramethylpyrazine inhibits the proliferation, invasiveness and migration of cervical cancer C33A cells by retarding the Hedgehog signaling pathway". Oncology Letters 23.2 (2022): 66.
Chicago
Ren, J., Cai, J., Wang, C."Tetramethylpyrazine inhibits the proliferation, invasiveness and migration of cervical cancer C33A cells by retarding the Hedgehog signaling pathway". Oncology Letters 23, no. 2 (2022): 66. https://doi.org/10.3892/ol.2022.13185
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