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Mutations in the helix αC of the catalytic domain from the EGFR affect its activity in cervical cancer cell lines

  • Authors:
    • Arturo Valle‑Mendiola
    • Ricardo Bustos‑Rodríguez
    • Vanihamin Domínguez‑Melendez
    • Octavio Zerecero‑Carreón
    • Adriana Gutiérrez‑Hoya
    • Benny Weiss‑Steider
    • Isabel Soto‑Cruz
  • View Affiliations / Copyright

    Affiliations: Molecular Oncology Laboratory, Cell Differentiation and Cancer Research Unit, UMIEZ Campus II, FES Zaragoza, National University of Mexico, Iztapalapa, Mexico City 09230, Mexico, Center for Studies and Health Services, Veracruz University, Veracruz 91700, Mexico
    Copyright: © Valle‑Mendiola et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 71
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    Published online on: January 5, 2022
       https://doi.org/10.3892/ol.2022.13191
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Abstract

The EGFR is a protein that belongs to the ErbB family of tyrosine kinase receptors. The EGFR is often overexpressed in human carcinomas. Amplification of the EGFR gene and mutations in the EGFR tyrosine kinase domain occur in patients with cancer. In cervical cancer, the expression level of the EGFR protein appears to directly associate with human papillomavirus infection. Our previous research demonstrated that in the cervical cancer cell lines, CALO and INBL, the EGFR is non‑phosphorylated. The aim of the current study was to analyze the catalytic activity of the isolated EGFR and the presence of mutations in the control region αC. Catalytic activity was assessed by a universal in vitro kinase assay using polyGluTyr as a substrate, and the proteins were visualized by western blotting. For mutation analysis, DNA from CALO and INBL cell lines was isolated, and PCR was used to amplify the exons corresponding to the helix αC in the EGFR. The PCR products were visualized by agarose gel electrophoresis. The bands were isolated using a Zymoclean Gel DNA Recovery kit and directly sequenced. The EGFR, which was isolated and analyzed using the in vitro kinase assay, had catalytic activity. The receptor contained some mutations in the helix αC of the catalytic domain in both cell lines. The observed changes in the amino acid sequence may induce a different spatial arrangement and, therefore, a different conformation, which may confer different activities to this receptor. Thus, it was concluded that non‑phosphorylated EGFR has catalytic activity, and it bears some amino acid changes in the helix αC of the catalytic domain in the CALO and INBL cells. These results suggest that the EGFR may function as an activator of other ErbB family receptors in these cervical cancer cells.
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1 

Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A and Bray F: Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 71:209–249. 2021. View Article : Google Scholar : PubMed/NCBI

2 

Bouvard V, Baan R, Straif K, Grosse Y, Secretan B, El Ghissassi F, Benbrahim-Tallaa L, Guha N, Freeman C, Galichet L, et al: A review of human carcinogens-Part B: Biological agents. Lancet Oncol. 10:321–322. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Zur Hausen H: Papillomaviruses and cancer: From basic studies to clinical application. Nat Rev Cancer. 2:342–350. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Munoz N, Bosch FX, de Sanjose S, Herrero R, Castellsaque X, Shah KV, Snijders PJ and Meijer CJ; International Agency for Research on Cancer Multicenter Cervical Cancer Study Group, : Epidemiologic classification of human papillomavirus types associated with cervical cancer. N Engl J Med. 348:518–527. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Soonthornthum T, Arias-Pulido H, Joste N, Lomo L, Muller C, Rutledge T and Verschraegen C: Epidermal growth factor receptor as a biomarker for cervical cancer. Ann Oncol. 22:2166–2178. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Stöppler MC, Straight SW, Tsao G, Schlegel R and Mccance DJ: The E5 gene of HPV16 enhances keratinocyte immortalization by full-length DNA. Virology. 223:251–254. 1996. View Article : Google Scholar : PubMed/NCBI

7 

Tomakidi P, Cheng H, Kohl A, Komposch G and Alonso A: Modulation of the epidermal growth factor receptor by the human papillomavirus type 16 E5 protein in raft cultures of human keratinocytes. Eur J Cell Biol. 79:407–412. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Jura N, Shan Y, Cao X, Shaw DE and Kuriyan J: Structural analysis of the catalytically inactive kinase domain of the human EGF receptor 3. Proc Natl Acad Sci USA. 106:21608–21613. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Sierke SL, Cheng K, Kim HH and Koland JG: Biochemical characterization of the protein tyrosine kinase homology domain of the ErbB3 (HER3) receptor protein. Biochem J. 322:757–763. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Wallasch C, Weiss FU, Niederfellner G, Jallal BA, Issing W and Ullrich A: Heregulin-dependent regulation of HER2/neu oncogenic signaling by heterodimerization with HER3. EMBO J. 14:4267–4275. 1995. View Article : Google Scholar : PubMed/NCBI

11 

Shi F, Telesco SE, Liu Y, Radhakrishnan R and Lemmon MA: ErbB3/HER3 intracellular domain is competent to bind ATP and catalyze autophosphorylation. Proc Natl Acad Sci USA. 107:7692–7697. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Lynch TJ, Bell DW, Sordella R, Gurubhagavatula S, Okimoto RA, Brannigan BW, Harris PL, Haserlat SM, Supko JG, Haluska FG, et al: Activating mutations in the epidermal growth factor underlying responsiveness of non-small-cell lung cancer to gefitinib. N Engl J Med. 350:2129–2139. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Iida K, Nakayama K, Rahman MT, Rahman M, Ishikawa M, Katagiri A, Yeasmin S, Otsuki Y, Kobayashi H, Nakayama S and Miyazaki K: EGFR gene amplification is related to adverse clinical outcomes in cervical squamous cell carcinoma, making the EGFR pathway a novel therapeutic target. Br J Cancer. 105:420–427. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Wolber L, Hess S, Bohlken H, Tennstedt P, Eulenburg C, Simon R, Gieseking F, Jaenicke F, Mahner S and Choschzinck M: EGFR gene copy number increase in vulvar carcinoma is linked with poor clinical outcome. J Clin Pathol. 65:133–139. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Li Q, Tang Y, Cheng X, Ji J, Zhang J and Zhou X: EGFR protein expression and gene amplification in squamous intraepithelial lesions and squamous cell carcinomas of the cervix. Int J Clin Exp Pathol. 7:733–741. 2014.PubMed/NCBI

16 

Conesa-Zamora P, Torres-Moreno D, Isaac MA and Pérez-Guillermo M: Gene amplification and immunohistochemical expression of ERBB2 and EGFR in cervical carcinogenesis. Correlation with cell-cycle markers and HPV presence. Exp Mol Pathol. 95:151–155. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Li Q, Cheng X, Ji J, Zhang J and Zhou X: Gene amplification of EGFR and its clinical significance in various cervical (lesions) lesions using cytology and FISH. Int J Clin Exp Pathol. 7:2477–2483. 2014.PubMed/NCBI

18 

Arias-Pulido H, Joste N, Chavez A, Muller CY, Dai D, Smith HO and Verschraegen CF: Absence of epidermal growth factor receptor mutations in cervical cancer. Int J Gynecol Cancer. 18:749–754. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Soto-Cruz I, Rangel-Corona R, Valle-Mendiola A, Moreno-Morales X, Santiago-Pérez R, Weiss-Steider B and Cáceres-Cortés JR: The tyrphostin B42 inhibits cell proliferation and HER-2 autophosphorylation in cervical carcinoma cell lines. Cancer Invest. 26:136–144. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Caceres-Cortes JR, Alvarado-Moreno JA, Waga K, Rangel-Corona R, Monroy-Garcia A, Rocha-Zavaleta L, Urdiales-Ramos J, Weiss-Steider B, Haman A, Hugo P, et al: Implication of tyrosine kinase receptor and steel factor in cell density-dependent growth in cervical cancers and leukemias. Cancer Res. 61:6281–6290. 2001.PubMed/NCBI

21 

Biasini M, Bienert S, Waterhouse A, Arnold K, Studer G, Schmidt T, Kiefer F, Cassarino TG, Bertoni M, Bordoli L and Schwede T: SWISS-MODEL: Modeling protein tertiary and quaternary structures using evolutionary information. Nucleic Acids Res. 42:W252–W258. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Arnold K, Bordoli L, Kopp J and Schwede T: The SWISS-MODEL workspace: A web-based environment for protein structure homology modeling. Bioinformatics. 22:195–201. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Benkert P, Biasini M and Schwede T: Toward the estimation of the absolute quality of individual protein structure models. Bioinformatics. 27:343–350. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Valle-Mendiola A, Weiss-Steider B, Rocha-Zavaleta L and Soto-Cruz I: IL-2 enhances cervical cancer cells proliferation and JAK3/STAT5 phosphorylation at low doses, while at high doses IL-2 has opposite effects. Cancer Invest. 32:115–125. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Yarden Y and Pines G: The ERBB network: At last, cancer therapy meets systems biology. Nat Rev Cancer. 12:553–563. 2012. View Article : Google Scholar : PubMed/NCBI

26 

London M and Gallo E: Epidermal growth factor receptor (EGFR) involvement in epitelial-derived cancers and its current antibody-based immunotherapies. Cell Biol Int. 44:1267–1282. 2020. View Article : Google Scholar : PubMed/NCBI

27 

Rosell R, Moran T, Queralt C, Porta R, Cardenal F, Camps C, Majem M, López-Vivanco G, Isla D, Provencio M, et al: Screening for epidermal growth factor receptor mutations in lung cancer. N Engl J Med. 361:958–977. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Ayati A, Moghimi S, Salarinejad S, Safavi M, Pouramiri B and Foroumadi A: A review on progression of epidermal growth factor receptor (EGFR) inhibitors as an efficient approach in cancer targeted therapy. Bioorg Chem. 99:1038112020. View Article : Google Scholar : PubMed/NCBI

29 

Anastasi S, Baietti MF, Frosi Y, Alema S and Segatto O: The evolutionarily conserved EBR module of RALT/MIG6 mediates suppression of the EGFR catalytic activity. Oncogene. 26:7833–7846. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Singh D, Attri BK, Gill RK and Bariwal J: Review on EGFR inhibitors: Critical update. Mini Rev Med Chem. 16:1134–1166. 2016. View Article : Google Scholar : PubMed/NCBI

31 

De Bondt HL, Rosenblatt J, Jancarik J, Jones HD, Morgan DO and Kim SH: Crystal structure of cyclin-dependent kinase 2. Nature. 363:595–602. 1993. View Article : Google Scholar : PubMed/NCBI

32 

Xu W, Doshi A, Lei M, Eck MJ and Harrison SC: Crystal structure of c-Src reveal features of its autoinhibitory mechanism. Mol Cell. 3:629–638. 1999. View Article : Google Scholar : PubMed/NCBI

33 

Deindl S, Kadlecek TA, Brdicka T, Cao X, Weiss A and Kuriyan J: Structural basis for the inhibition of tyrosine kinase activity of ZAP-70. Cell. 129:735–746. 2007. View Article : Google Scholar : PubMed/NCBI

34 

Wood ER, Truesdale AT, McDonald OB, Yuan D, Hassell A, Dickerson SH, Ellis B, Pennisi C, Horne E, Lackey K, et al: A unique structure for epidermal growth factor receptor bound to GW572016 (Lapatinib): relationship among protein conformation, inhibitor off-rate, and receptor activity in tumor cells. Cancer Res. 64:6652–6659. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Manning G, Whyte DB, Martinez R, Hunter T and Sudarsanam S: The protein kinase complement of the human genome. Science. 298:1912–1934. 2002. View Article : Google Scholar : PubMed/NCBI

36 

Guy PM, Platko JV, Cantley LC, Cerione RA and Carraway KL III: Insect cell-expressed p180erbB3 possesses an impaired tyrosine kinase activity. Proc Natl Acad Sci USA. 91:8132–8136. 1994. View Article : Google Scholar : PubMed/NCBI

37 

Zhang X, Gureasko J, Shen K, Cole PA and Kuriyan J: An allosteric mechanism for activation of the kinase domain of epidermal growth factor receptor. Cell. 125:1137–1149. 2006. View Article : Google Scholar : PubMed/NCBI

38 

Jeffrey PD, Russo AA, Polyak K, Gibbs E, Hurwitz J, Massague J and Pavletich NP: Mechanism of CDK activation revealed by structure of a cyclinA-CDK2 complex. Nature. 376:313–320. 1995. View Article : Google Scholar : PubMed/NCBI

39 

Baselga J and Swain SM: Novel anticancer targets: Revisiting ERBB2 and discovering ERBB3. Nat Rev Cancer. 9:463–475. 2009. View Article : Google Scholar : PubMed/NCBI

40 

Sergina NV, Rausch M, Wang D, Blair J, Hann B, Shokat KM and Moasser MM: Escape from HER-family tyrosine kinase inhibitor therapy by the kinase-inactive HER3. Nature. 445:437–441. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Valle‑Mendiola A, Bustos‑Rodríguez R, Domínguez‑Melendez V, Zerecero‑Carreón O, Gutiérrez‑Hoya A, Weiss‑Steider B and Soto‑Cruz I: Mutations in the helix αC of the catalytic domain from the EGFR affect its activity in cervical cancer cell lines. Oncol Lett 23: 71, 2022.
APA
Valle‑Mendiola, A., Bustos‑Rodríguez, R., Domínguez‑Melendez, V., Zerecero‑Carreón, O., Gutiérrez‑Hoya, A., Weiss‑Steider, B., & Soto‑Cruz, I. (2022). Mutations in the helix αC of the catalytic domain from the EGFR affect its activity in cervical cancer cell lines. Oncology Letters, 23, 71. https://doi.org/10.3892/ol.2022.13191
MLA
Valle‑Mendiola, A., Bustos‑Rodríguez, R., Domínguez‑Melendez, V., Zerecero‑Carreón, O., Gutiérrez‑Hoya, A., Weiss‑Steider, B., Soto‑Cruz, I."Mutations in the helix αC of the catalytic domain from the EGFR affect its activity in cervical cancer cell lines". Oncology Letters 23.2 (2022): 71.
Chicago
Valle‑Mendiola, A., Bustos‑Rodríguez, R., Domínguez‑Melendez, V., Zerecero‑Carreón, O., Gutiérrez‑Hoya, A., Weiss‑Steider, B., Soto‑Cruz, I."Mutations in the helix αC of the catalytic domain from the EGFR affect its activity in cervical cancer cell lines". Oncology Letters 23, no. 2 (2022): 71. https://doi.org/10.3892/ol.2022.13191
Copy and paste a formatted citation
x
Spandidos Publications style
Valle‑Mendiola A, Bustos‑Rodríguez R, Domínguez‑Melendez V, Zerecero‑Carreón O, Gutiérrez‑Hoya A, Weiss‑Steider B and Soto‑Cruz I: Mutations in the helix αC of the catalytic domain from the EGFR affect its activity in cervical cancer cell lines. Oncol Lett 23: 71, 2022.
APA
Valle‑Mendiola, A., Bustos‑Rodríguez, R., Domínguez‑Melendez, V., Zerecero‑Carreón, O., Gutiérrez‑Hoya, A., Weiss‑Steider, B., & Soto‑Cruz, I. (2022). Mutations in the helix αC of the catalytic domain from the EGFR affect its activity in cervical cancer cell lines. Oncology Letters, 23, 71. https://doi.org/10.3892/ol.2022.13191
MLA
Valle‑Mendiola, A., Bustos‑Rodríguez, R., Domínguez‑Melendez, V., Zerecero‑Carreón, O., Gutiérrez‑Hoya, A., Weiss‑Steider, B., Soto‑Cruz, I."Mutations in the helix αC of the catalytic domain from the EGFR affect its activity in cervical cancer cell lines". Oncology Letters 23.2 (2022): 71.
Chicago
Valle‑Mendiola, A., Bustos‑Rodríguez, R., Domínguez‑Melendez, V., Zerecero‑Carreón, O., Gutiérrez‑Hoya, A., Weiss‑Steider, B., Soto‑Cruz, I."Mutations in the helix αC of the catalytic domain from the EGFR affect its activity in cervical cancer cell lines". Oncology Letters 23, no. 2 (2022): 71. https://doi.org/10.3892/ol.2022.13191
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