Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
February-2022 Volume 23 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2022 Volume 23 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Immune‑related gene expression in skin, inflamed and keloid tissue from patients with keloids

  • Authors:
    • Mengjie Shan
    • Hao Liu
    • Kexin Song
    • Shu Liu
    • Yan Hao
    • Youbin Wang
  • View Affiliations / Copyright

    Affiliations: Department of Plastic Surgery, Peking Union Medical College Hospital, Beijing 100730, P.R. China, Department of General Surgery, Civil Aviation General Hospital, Beijing 100123, P.R. China
    Copyright: © Shan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 72
    |
    Published online on: January 5, 2022
       https://doi.org/10.3892/ol.2022.13192
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Keloids are a tumor‑like fibroproliferative skin disease that could cause disfigurement and disability. The pathological mechanisms underlying this condition remain unclear, particularly the progression from normal healthy skin to inflammatory skin tissue, then keloid. In the present study, three immune‑related gene expression profiling datasets, were obtained from normal skin tissue (N group), inflamed tissue (I group) and keloid tissue samples from patients with keloids (K group). This sample grouping represents the primary steps of keloid formation, from normal to inflammatory, and finally to keloid tissue. The expression levels of immune‑related genes were analyzed, and the differentially expressed genes (DEGs) between the three groups were compared. Protein‑protein interaction networks were established using Cytoscape. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were carried out to determine the main functions associated with the DEGs and keloid‑­associated pathways. The results identified hub genes in the N and I groups, including C‑C motif chemokine receptor (CCR) 1, CCR7, CD40 ligand, C‑X‑C motif chemokine ligand 9, IL‑6 and IL‑10. The hub genes in the I and the K groups included IL‑10, IL‑6, IL‑13 and CD86. The expression levels of these genes were verified using reverse transcription‑­quantitative PCR. The results demonstrated that IL‑6 expression levels were significantly increased in the I group compared with the N group (P=0.0111). CCR7 levels significantly differed between all three groups (P<0.017). The results of GO analysis suggested that the hub genes in the I and N groups may be associated with ‘regulation of lymphocyte activation’ and ‘T‑cell activation’. Similar results were also observed between the I and K groups, which may play an important role in keloid initiation and formation. In conclusion, CCR7, IL‑10 and IL‑6 may be important in keloid initiation and formation. These findings provided insight into the pathogenesis of keloids and may help identify novel immune‑related therapeutic targets for this condition.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

Figure 9

View References

1 

Brown JJ, Ollier W, Arscott G, Ke X, Lamb J, Day P and Bayat A: Genetic susceptibility to keloid scarring: SMAD gene SNP frequencies in Afro-caribbeans. Exp Dermatol. 17:610–613. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Chung S, Nakashima M, Zembutsu H and Nakamura Y: Possible involvement of NEDD4 in keloid formation; its critical role in fibroblast proliferation and collagen production. Proc Jpn Acad Ser B Phys Biol Sci. 87:563–573. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Glass DA II: Current understanding of the genetic causes of keloid formation. J Investig Dermatol Symp Proc. 18:S50–S53. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Tsai CH and Ogawa R: Keloid research: Current status and future directions. Scars Burn Heal. 5:20595131198686592019.PubMed/NCBI

5 

Song KX, Liu S, Zhang MZ, Liang WZ, Liu H, Dong XH, Wang YB and Wang XJ: Hyperbaric oxygen therapy improves the effect of keloid surgery and radiotherapy by reducing the recurrence rate. J Zhejiang Univ Sci B. 19:853–862. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Ogawa R: Keloid and hypertrophic scars are the result of chronic inflammation in the reticular dermis. Int J Mol Sci. 18:6062017. View Article : Google Scholar : PubMed/NCBI

7 

Diaz A, Tan K, He H, Xu H, Cueto I, Pavel AB, Krueger JG and Guttman-Yassky E: Keloid lesions show increased IL-4/IL-13 signaling and respond to Th2-targeting dupilumab therapy. J Eur Acad Dermatol Venereol. 34:e161–e164. 2020. View Article : Google Scholar : PubMed/NCBI

8 

Shi CK, Zhao YP, Ge P and Huang GB: Therapeutic effect of interleukin-10 in keloid fibroblasts by suppression of TGF-β/Smad pathway. Eur Rev Med Pharmacol Sci. 23:9085–9092. 2019.PubMed/NCBI

9 

Gankande TU, Wood FM, Edgar DW, Duke JM, DeJong HM, Henderson AE and Wallace HJ: A modified vancouver scar scale linked with TBSA (mVSS-TBSA): Inter-rater reliability of an innovative burn scar assessment method. Burns. 39:1142–1149. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Wang CH, Shan MJ, Liu H, Hao Y, Song KX, Wu HW, Meng T, Feng C, Qi Z, Wang Z and Wang YB: Hyperbaric oxygen treatment on keloid tumor immune gene expression. Chin Med J (Engl). 134:2205–2213. 2021.PubMed/NCBI

11 

Privé F, Luu K, Vilhjálmsson BJ and Blum M: Performing highly efficient genome scans for local adaptation with r package pcadapt version 4. Mol Biol Evol. 37:2153–2154. 2020. View Article : Google Scholar : PubMed/NCBI

12 

Kanehisa M and Goto S: KEGG: Kyoto encyclopedia of genes and genomes. Nucleic Acids Res. 28:27–30. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Huang DW, Sherman BT and Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 4:44–57. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Szklarczyk D, Gable AL, Lyon D, Junge A, Wyder S, Huerta-Cepas J, Simonovic M, Doncheva NT, Morris JH and Bork P: STRING v11: Protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets. Nucleic Acids Res. 47:D607–D613. 2019. View Article : Google Scholar : PubMed/NCBI

15 

Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B and Ideker T: Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Res. 13:2498–2504. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Ni M, Liu X, Wu J, Zhang D, Tian J, Wang T, Liu S, Meng Z, Wang K, Duan X, et al: Identification of candidate biomarkers correlated with the pathogenesis and prognosis of non-small cell lung cancer via integrated bioinformatics analysis. Front Genet. 9:4692018. View Article : Google Scholar : PubMed/NCBI

17 

Yan J, Zuo G, Sherchan P, Huang L, Ocak U, Xu W Travis Z, Wang W, Zhang J and Tang J: CCR1 Activation Promotes Neuroinflammation Through CCR1/TPR1/ERK1/2 Signaling Pathway After Intracerebral Hemorrhage in Mice. Neurotherapeutics. 17:1170–1183. 2020. View Article : Google Scholar : PubMed/NCBI

18 

Chin CH, Chen SH, Wu HH, Ho CW, Ko MT and Lin CY: cytoHubba: Identifying hub objects and sub-networks from complex interactome. BMC Syst Biol. 8 (Suppl 4):S112014. View Article : Google Scholar : PubMed/NCBI

19 

Song X, Du R, Gui H, Zhou M, Zhong W, Mao C and Ma J: Identification of potential hub genes related to the progression and prognosis of hepatocellular carcinoma through integrated bioinformatics analysis. Oncol Rep. 43:133–146. 2020.PubMed/NCBI

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-(Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Zhang Q, Yamaza T, Kelly AP, Shi S, Wang S, Brown J, Wang L, French SW, Shi S and Le AD: Tumor-like stem cells derived from human keloid are governed by the inflammatory niche driven by IL-17/IL-6 axis. PLoS One. 4:e77982009. View Article : Google Scholar : PubMed/NCBI

22 

Limandjaja GC, Niessen FB, Scheper RJ and Gibbs S: The keloid disorder: Heterogeneity, histopathology, mechanisms and models. Front Cell Dev Biol. 8:3602020. View Article : Google Scholar : PubMed/NCBI

23 

van den Broek LJ, Limandjaja GC, Niessen FB and Gibbs S: Human hypertrophic and keloid scar models: Principles, limitations and future challenges from a tissue engineering perspective. Exp Dermatol. 23:382–386. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Tan S, Khumalo N and Bayat A: Understanding keloid pathobiology from a quasi-neoplastic perspective: Less of a scar and more of a chronic inflammatory disease with cancer-like tendencies. Front Immunol. 10:18102019. View Article : Google Scholar : PubMed/NCBI

25 

Karnell JL, Rieder SA, Ettinger R and Kolbeck R: Targeting the CD40-CD40L pathway in autoimmune diseases: Humoral immunity and beyond. Adv Drug Deliv Rev. 141:92–103. 2019. View Article : Google Scholar : PubMed/NCBI

26 

Tokunaga R, Zhang W, Naseem M, Puccini A, Berger MD, Soni S, McSkane M, Baba H and Lenz HJ: CXCL9, CXCL10, CXCL11/CXCR3 axis for immune activation-A target for novel cancer therapy. Cancer Treat Rev. 63:40–47. 2018. View Article : Google Scholar : PubMed/NCBI

27 

Zhong L, Bian L, Lyu J, Jin H, Liu Z, Lyu L and Lu D: Identification and integrated analysis of microRNA expression profiles in keloid. J Cosmet Dermatol. 17:917–924. 2018. View Article : Google Scholar : PubMed/NCBI

28 

Ghazizadeh M: Essential role of IL-6 signaling pathway in keloid pathogenesis. J Nippon Med Sch. 74:11–22. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Jin Q, Gui L, Niu F, Yu B, Lauda N, Liu J, Mao X and Chen Y: Macrophages in keloid are potent at promoting the differentiation and function of regulatory T cells. Exp Cell Res. 362:472–476. 2018. View Article : Google Scholar : PubMed/NCBI

30 

Suzuki M, Yokota M, Matsumoto T and Ozaki S: Synergic effects of CD40 and CD86 silencing in dendritic cells on the control of allergic diseases. Int Arch Allergy Immunol. 177:87–96. 2018. View Article : Google Scholar : PubMed/NCBI

31 

Mburu YK, Wang J, Wood MA, Walker WH and Ferris RL: CCR7 mediates inflammation-associated tumor progression. Immunol Res. 36:61–72. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Huang H, Fu S and Liu D: Detection and analysis of the hedgehog signaling pathway-related long non-coding RNA (lncRNA) expression profiles in keloid. Med Sci Monit. 24:9032–9044. 2018. View Article : Google Scholar : PubMed/NCBI

33 

Spangler JB, Moraga I, Mendoza JL and Garcia KC: Insights into cytokine-receptor interactions from cytokine engineering. Annu Rev Immunol. 33:139–167. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Shan M, Liu H, Song K, Liu S, Hao Y and Wang Y: Immune‑related gene expression in skin, inflamed and keloid tissue from patients with keloids. Oncol Lett 23: 72, 2022.
APA
Shan, M., Liu, H., Song, K., Liu, S., Hao, Y., & Wang, Y. (2022). Immune‑related gene expression in skin, inflamed and keloid tissue from patients with keloids. Oncology Letters, 23, 72. https://doi.org/10.3892/ol.2022.13192
MLA
Shan, M., Liu, H., Song, K., Liu, S., Hao, Y., Wang, Y."Immune‑related gene expression in skin, inflamed and keloid tissue from patients with keloids". Oncology Letters 23.2 (2022): 72.
Chicago
Shan, M., Liu, H., Song, K., Liu, S., Hao, Y., Wang, Y."Immune‑related gene expression in skin, inflamed and keloid tissue from patients with keloids". Oncology Letters 23, no. 2 (2022): 72. https://doi.org/10.3892/ol.2022.13192
Copy and paste a formatted citation
x
Spandidos Publications style
Shan M, Liu H, Song K, Liu S, Hao Y and Wang Y: Immune‑related gene expression in skin, inflamed and keloid tissue from patients with keloids. Oncol Lett 23: 72, 2022.
APA
Shan, M., Liu, H., Song, K., Liu, S., Hao, Y., & Wang, Y. (2022). Immune‑related gene expression in skin, inflamed and keloid tissue from patients with keloids. Oncology Letters, 23, 72. https://doi.org/10.3892/ol.2022.13192
MLA
Shan, M., Liu, H., Song, K., Liu, S., Hao, Y., Wang, Y."Immune‑related gene expression in skin, inflamed and keloid tissue from patients with keloids". Oncology Letters 23.2 (2022): 72.
Chicago
Shan, M., Liu, H., Song, K., Liu, S., Hao, Y., Wang, Y."Immune‑related gene expression in skin, inflamed and keloid tissue from patients with keloids". Oncology Letters 23, no. 2 (2022): 72. https://doi.org/10.3892/ol.2022.13192
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team