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Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9

  • Authors:
    • Ryutaro Shimizu
    • Takahito Ohira
    • Takuki Yagyu
    • Tetsuya Yumioka
    • Noriya Yamaguchi
    • Hideto Iwamoto
    • Shuichi Morizane
    • Katsuya Hikita
    • Masashi Honda
    • Atsushi Takenaka
    • Hiroyuki Kugoh
  • View Affiliations / Copyright

    Affiliations: Division of Urology, Department of Surgery, Tottori University Faculty of Medicine, Yonago, Tottori 683‑8504, Japan, Department of Molecular and Cellular Biology, Division of Genome and Cellular Function, Tottori University, Yonago, Tottori 683‑8503, Japan
    Copyright: © Shimizu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 92
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    Published online on: January 27, 2022
       https://doi.org/10.3892/ol.2022.13212
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Abstract

Bladder cancer is divided into two molecular subtypes, luminal and basal, which form papillary and nodular tumors, respectively, and are identifiable by gene expression profiling. Although loss of heterozygosity (LOH) of the long arm of human chromosome 9 (9q) has been observed in the early development of both types of bladder cancer, the functional significance of LOH remains to be clarified. The present study introduced human chromosome 9q into basal bladder cancer cell line, SCaBER, using microcell‑mediated chromosome transfer to investigate the effect of LOH of 9q on molecular bladder cancer subtypes. These cells demonstrated decreased proliferation and migration capacity compared with parental and control cells. Conversely, transfer of human chromosome 4 did not change the cell phenotype. Expression level of peroxisome proliferator‑activated receptor (PPAR)γ, a marker of luminal type, increased 3.0‑4.4 fold in SCaBER cells altered with 9q compared with parental SCaBER cells. Furthermore, the expression levels of tumor suppressor PTEN, which regulates PPARγ, also increased in 9q‑altered cells. These results suggested that human chromosome 9q may carry regulatory genes for PPARγ that are involved in the progression of neoplastic transformation of bladder cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Shimizu R, Ohira T, Yagyu T, Yumioka T, Yamaguchi N, Iwamoto H, Morizane S, Hikita K, Honda M, Takenaka A, Takenaka A, et al: Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9. Oncol Lett 23: 92, 2022.
APA
Shimizu, R., Ohira, T., Yagyu, T., Yumioka, T., Yamaguchi, N., Iwamoto, H. ... Kugoh, H. (2022). Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9. Oncology Letters, 23, 92. https://doi.org/10.3892/ol.2022.13212
MLA
Shimizu, R., Ohira, T., Yagyu, T., Yumioka, T., Yamaguchi, N., Iwamoto, H., Morizane, S., Hikita, K., Honda, M., Takenaka, A., Kugoh, H."Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9". Oncology Letters 23.3 (2022): 92.
Chicago
Shimizu, R., Ohira, T., Yagyu, T., Yumioka, T., Yamaguchi, N., Iwamoto, H., Morizane, S., Hikita, K., Honda, M., Takenaka, A., Kugoh, H."Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9". Oncology Letters 23, no. 3 (2022): 92. https://doi.org/10.3892/ol.2022.13212
Copy and paste a formatted citation
x
Spandidos Publications style
Shimizu R, Ohira T, Yagyu T, Yumioka T, Yamaguchi N, Iwamoto H, Morizane S, Hikita K, Honda M, Takenaka A, Takenaka A, et al: Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9. Oncol Lett 23: 92, 2022.
APA
Shimizu, R., Ohira, T., Yagyu, T., Yumioka, T., Yamaguchi, N., Iwamoto, H. ... Kugoh, H. (2022). Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9. Oncology Letters, 23, 92. https://doi.org/10.3892/ol.2022.13212
MLA
Shimizu, R., Ohira, T., Yagyu, T., Yumioka, T., Yamaguchi, N., Iwamoto, H., Morizane, S., Hikita, K., Honda, M., Takenaka, A., Kugoh, H."Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9". Oncology Letters 23.3 (2022): 92.
Chicago
Shimizu, R., Ohira, T., Yagyu, T., Yumioka, T., Yamaguchi, N., Iwamoto, H., Morizane, S., Hikita, K., Honda, M., Takenaka, A., Kugoh, H."Activation of PPARγ in bladder cancer via introduction of the long arm of human chromosome 9". Oncology Letters 23, no. 3 (2022): 92. https://doi.org/10.3892/ol.2022.13212
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