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Preferential radiosensitization to glioblastoma cancer stem cell‑like cells by a Hsp90 inhibitor, N‑vinylpyrrolidone‑AUY922

  • Authors:
    • Toshiaki Tani
    • Naomi Tojo
    • Ken Ohnishi
  • View Affiliations / Copyright

    Affiliations: Radiological Technology Section, QST Hospital, National Institutes for Quantum and Radiological Science and Technology, Chiba 263‑8555, Japan, Department of Biology, Ibaraki Prefectural University of Health Sciences, Inashiki, Ibaraki 300‑0394, Japan
    Copyright: © Tani et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 102
    |
    Published online on: January 31, 2022
       https://doi.org/10.3892/ol.2022.13222
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Abstract

The present study examined the radiosensitization induced by a heat shock protein 90 inhibitor, N‑vinylpyrrolidone (NVP)‑AUY922, in CD133‑positive cells in a hypoxic area of T98G spheroids. CD133‑positive cells that are induced in the hypoxic microenvironment of spheroids have previously been reported to exhibit cancer stem cell‑like properties. The present study used CD133‑positive cells from a glioblastoma cell line (T98G) as cancer stem cell‑like cells. CD133‑positive and negative cells were sorted from T98G spheroids using fluorescence‑activated cell sorting and used for colony formation assay. Colony formation assay results indicated that NVP‑AUY922 enhanced radiosensitivity more strongly in CD133‑positive cells compared with CD133‑negative cells. This result showed that NVP‑AUY922 was a preferential radiosensitization candidate targeting glioblastoma cancer stem cells. The mechanisms underlying radiosensitization by NVP‑AUY922 are discussed in relation to the properties of cancer stem cells. Overall, HIF‑1α inhibition by NVP‑AUY922 may induce higher sensitization of cancer stem cells to radiation.
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Copy and paste a formatted citation
Spandidos Publications style
Tani T, Tojo N and Ohnishi K: Preferential radiosensitization to glioblastoma cancer stem cell‑like cells by a Hsp90 inhibitor, N‑vinylpyrrolidone‑AUY922. Oncol Lett 23: 102, 2022.
APA
Tani, T., Tojo, N., & Ohnishi, K. (2022). Preferential radiosensitization to glioblastoma cancer stem cell‑like cells by a Hsp90 inhibitor, N‑vinylpyrrolidone‑AUY922. Oncology Letters, 23, 102. https://doi.org/10.3892/ol.2022.13222
MLA
Tani, T., Tojo, N., Ohnishi, K."Preferential radiosensitization to glioblastoma cancer stem cell‑like cells by a Hsp90 inhibitor, N‑vinylpyrrolidone‑AUY922". Oncology Letters 23.3 (2022): 102.
Chicago
Tani, T., Tojo, N., Ohnishi, K."Preferential radiosensitization to glioblastoma cancer stem cell‑like cells by a Hsp90 inhibitor, N‑vinylpyrrolidone‑AUY922". Oncology Letters 23, no. 3 (2022): 102. https://doi.org/10.3892/ol.2022.13222
Copy and paste a formatted citation
x
Spandidos Publications style
Tani T, Tojo N and Ohnishi K: Preferential radiosensitization to glioblastoma cancer stem cell‑like cells by a Hsp90 inhibitor, N‑vinylpyrrolidone‑AUY922. Oncol Lett 23: 102, 2022.
APA
Tani, T., Tojo, N., & Ohnishi, K. (2022). Preferential radiosensitization to glioblastoma cancer stem cell‑like cells by a Hsp90 inhibitor, N‑vinylpyrrolidone‑AUY922. Oncology Letters, 23, 102. https://doi.org/10.3892/ol.2022.13222
MLA
Tani, T., Tojo, N., Ohnishi, K."Preferential radiosensitization to glioblastoma cancer stem cell‑like cells by a Hsp90 inhibitor, N‑vinylpyrrolidone‑AUY922". Oncology Letters 23.3 (2022): 102.
Chicago
Tani, T., Tojo, N., Ohnishi, K."Preferential radiosensitization to glioblastoma cancer stem cell‑like cells by a Hsp90 inhibitor, N‑vinylpyrrolidone‑AUY922". Oncology Letters 23, no. 3 (2022): 102. https://doi.org/10.3892/ol.2022.13222
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