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Effects of 2,2',4'‑trihydroxychalcone on the proliferation, metastasis and apoptosis of A549 human lung cancer cells

  • Authors:
    • Jia-Lin Sun
    • Zhan-Qi Cao
    • Shi-Wei Sun
    • Zhong-Hua Sun
    • Shu-Hong Sun
    • Jin-Feng Ye
    • Ping Leng
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266000, P.R. China, Department of Natural Medicine and Pharmacognosy, School of Pharmacy, Qingdao University, Qingdao, Shandong 266071, P.R. China, School of Medicine and Pharmacy, Ocean University of China, Qingdao, Shandong 266100, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 116
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    Published online on: February 9, 2022
       https://doi.org/10.3892/ol.2022.13236
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Abstract

The aim of the present study was to evaluate the antitumor effects of 2,2',4'‑trihydroxychalcone (7a) on the A549 human lung cancer cell line. A549 cells were treated with different concentrations of 7a for different time periods. Cells without 7a were used as the negative control group. Cell proliferation, invasion, vasculogenic mimicry (VM) formation, heterogeneous adhesion and apoptosis were measured using Cell Counting Kit‑8, Transwell invasion, VM, adhesion and flow cytometric assays, respectively. In addition, the expression of related proteins was determined using western blot analysis or ELISA. The present study found that 7a had a significant inhibitory effect on the survival rate of the A549 lung cancer cells but almost no effect on BEAS‑2B human lung epithelial cells or human venous endothelial cells. The migration rate, VM length, invasion rate and heterogeneous adhesion number of cells treated with 7a significantly decreased as the concentration increased, while the apoptosis rate increased. Western blot analysis showed that 7a treatment significantly increased the expression levels of E‑cadherin, cleaved poly (ADP‑ribose) polymerase, Bax and caspase‑3 and simultaneously decreased the expression levels of metalloproteinase‑2/9, Bcl‑2, phosphorylated (p)‑PI3K, p‑AKT, p‑mTOR, vascular endothelial growth factor (VEGF), E‑selectin and N‑cadherin. At the same time, the ELISA results showed that the level of the pro‑angiogenic factor VEGF in the culture media was reduced in the presence of 7a. In addition, 7a could also reduce the nuclear NF‑κB protein expression, which could inhibit the gene transcription of tumor apoptosis and metastasis‑related proteins. Therefore, 7a may exert inhibitory effects on A549 cells by inhibiting cell proliferation, migration, VM formation and heterogeneous adhesion, as well as by inducing apoptosis through the suppression of the PI3K/AKT/NF‑κB signaling pathway; these findings suggested that 7a may be a promising agent for the treatment of lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Sun J, Cao Z, Sun S, Sun Z, Sun S, Ye J and Leng P: Effects of 2,2',4'‑trihydroxychalcone on the proliferation, metastasis and apoptosis of A549 human lung cancer cells. Oncol Lett 23: 116, 2022.
APA
Sun, J., Cao, Z., Sun, S., Sun, Z., Sun, S., Ye, J., & Leng, P. (2022). Effects of 2,2',4'‑trihydroxychalcone on the proliferation, metastasis and apoptosis of A549 human lung cancer cells. Oncology Letters, 23, 116. https://doi.org/10.3892/ol.2022.13236
MLA
Sun, J., Cao, Z., Sun, S., Sun, Z., Sun, S., Ye, J., Leng, P."Effects of 2,2',4'‑trihydroxychalcone on the proliferation, metastasis and apoptosis of A549 human lung cancer cells". Oncology Letters 23.4 (2022): 116.
Chicago
Sun, J., Cao, Z., Sun, S., Sun, Z., Sun, S., Ye, J., Leng, P."Effects of 2,2',4'‑trihydroxychalcone on the proliferation, metastasis and apoptosis of A549 human lung cancer cells". Oncology Letters 23, no. 4 (2022): 116. https://doi.org/10.3892/ol.2022.13236
Copy and paste a formatted citation
x
Spandidos Publications style
Sun J, Cao Z, Sun S, Sun Z, Sun S, Ye J and Leng P: Effects of 2,2',4'‑trihydroxychalcone on the proliferation, metastasis and apoptosis of A549 human lung cancer cells. Oncol Lett 23: 116, 2022.
APA
Sun, J., Cao, Z., Sun, S., Sun, Z., Sun, S., Ye, J., & Leng, P. (2022). Effects of 2,2',4'‑trihydroxychalcone on the proliferation, metastasis and apoptosis of A549 human lung cancer cells. Oncology Letters, 23, 116. https://doi.org/10.3892/ol.2022.13236
MLA
Sun, J., Cao, Z., Sun, S., Sun, Z., Sun, S., Ye, J., Leng, P."Effects of 2,2',4'‑trihydroxychalcone on the proliferation, metastasis and apoptosis of A549 human lung cancer cells". Oncology Letters 23.4 (2022): 116.
Chicago
Sun, J., Cao, Z., Sun, S., Sun, Z., Sun, S., Ye, J., Leng, P."Effects of 2,2',4'‑trihydroxychalcone on the proliferation, metastasis and apoptosis of A549 human lung cancer cells". Oncology Letters 23, no. 4 (2022): 116. https://doi.org/10.3892/ol.2022.13236
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