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Biological response of adrenal carcinoma and melanoma cells to mitotane treatment

  • Authors:
    • Ewelina Stelcer
    • Hanna Komarowska
    • Karol Jopek
    • Agnieszka Żok
    • Dariusz Iżycki
    • Agnieszka Malińska
    • Beata Szczepaniak
    • Zhanat Komekbai
    • Marek Karczewski
    • Tomasz Wierzbicki
    • Wiktoria Maria Suchorska
    • Marek Ruchała
    • Marcin Ruciński
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, Poznan University of Medical Sciences, 61‑001 Poznan, Poland, Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, 60‑355 Poznan, Poland, Division of Philosophy of Medicine and Bioethics, Department of Social Sciences and Humanities, Poznan University of Medical Sciences, 60‑806 Poznan, Poland, Department of Cancer Immunology, Poznan University of Medical Sciences, 61‑866 Poznan, Poland, Department of Histology, West Kazakhstan Marat Ospanov Medical University, Aktobe 030019, Kazakhstan, Department of General and Transplantation Surgery, Poznan University of Medical Sciences, 60‑355 Poznan, Poland, Department of General, Endocrinological and Gastroenterological Surgery, Poznan University of Medical Sciences, 60‑355 Poznan, Poland, Radiobiology Laboratory, Greater Poland Cancer Centre, 61‑866 Poznan, Poland
    Copyright: © Stelcer et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 120
    |
    Published online on: February 10, 2022
       https://doi.org/10.3892/ol.2022.13240
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Abstract

A previous case report described an adrenal incidentaloma initially misdiagnosed as adrenocortical carcinoma (ACC), which was treated with mitotane. The final diagnosis was metastatic melanoma of unknown primary origin. However, the patient developed rapid disease progression after mitotane withdrawal, suggesting a protective role for mitotane in a non‑adrenal‑derived tumor. The aim of the present study was to determine the biological response of primary melanoma cells obtained from that patient, and that of other established melanoma and ACC cell lines, to mitotane treatment using a proliferation assay, flow cytometry, quantitative PCR and microarrays. Although mitotane inhibited the proliferation of both ACC and melanoma cells, its role in melanoma treatment appears to be limited. Flow cytometry analysis and transcriptomic studies indicated that the ACC cell line was highly responsive to mitotane treatment, while the primary melanoma cells showed a moderate response in vitro. Mitotane modified the activity of several key biological processes, including ‘mitotic nuclear division’, ‘DNA repair’, ‘angiogenesis’ and ‘negative regulation of ERK1 and ERK2 cascade’. Mitotane administration led to elevated levels of DNA double‑strand breaks, necrosis and apoptosis. The present study provides a comprehensive insight into the biological response of mitotane‑treated cells at the molecular level. Notably, the present findings offer new knowledge on the effects of mitotane on ACC and melanoma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Stelcer E, Komarowska H, Jopek K, Żok A, Iżycki D, Malińska A, Szczepaniak B, Komekbai Z, Karczewski M, Wierzbicki T, Wierzbicki T, et al: Biological response of adrenal carcinoma and melanoma cells to mitotane treatment. Oncol Lett 23: 120, 2022.
APA
Stelcer, E., Komarowska, H., Jopek, K., Żok, A., Iżycki, D., Malińska, A. ... Ruciński, M. (2022). Biological response of adrenal carcinoma and melanoma cells to mitotane treatment. Oncology Letters, 23, 120. https://doi.org/10.3892/ol.2022.13240
MLA
Stelcer, E., Komarowska, H., Jopek, K., Żok, A., Iżycki, D., Malińska, A., Szczepaniak, B., Komekbai, Z., Karczewski, M., Wierzbicki, T., Suchorska, W. M., Ruchała, M., Ruciński, M."Biological response of adrenal carcinoma and melanoma cells to mitotane treatment". Oncology Letters 23.4 (2022): 120.
Chicago
Stelcer, E., Komarowska, H., Jopek, K., Żok, A., Iżycki, D., Malińska, A., Szczepaniak, B., Komekbai, Z., Karczewski, M., Wierzbicki, T., Suchorska, W. M., Ruchała, M., Ruciński, M."Biological response of adrenal carcinoma and melanoma cells to mitotane treatment". Oncology Letters 23, no. 4 (2022): 120. https://doi.org/10.3892/ol.2022.13240
Copy and paste a formatted citation
x
Spandidos Publications style
Stelcer E, Komarowska H, Jopek K, Żok A, Iżycki D, Malińska A, Szczepaniak B, Komekbai Z, Karczewski M, Wierzbicki T, Wierzbicki T, et al: Biological response of adrenal carcinoma and melanoma cells to mitotane treatment. Oncol Lett 23: 120, 2022.
APA
Stelcer, E., Komarowska, H., Jopek, K., Żok, A., Iżycki, D., Malińska, A. ... Ruciński, M. (2022). Biological response of adrenal carcinoma and melanoma cells to mitotane treatment. Oncology Letters, 23, 120. https://doi.org/10.3892/ol.2022.13240
MLA
Stelcer, E., Komarowska, H., Jopek, K., Żok, A., Iżycki, D., Malińska, A., Szczepaniak, B., Komekbai, Z., Karczewski, M., Wierzbicki, T., Suchorska, W. M., Ruchała, M., Ruciński, M."Biological response of adrenal carcinoma and melanoma cells to mitotane treatment". Oncology Letters 23.4 (2022): 120.
Chicago
Stelcer, E., Komarowska, H., Jopek, K., Żok, A., Iżycki, D., Malińska, A., Szczepaniak, B., Komekbai, Z., Karczewski, M., Wierzbicki, T., Suchorska, W. M., Ruchała, M., Ruciński, M."Biological response of adrenal carcinoma and melanoma cells to mitotane treatment". Oncology Letters 23, no. 4 (2022): 120. https://doi.org/10.3892/ol.2022.13240
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