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Article Open Access

TPX2 is a prognostic marker and promotes cell proliferation in neuroblastoma

  • Authors:
    • Yuhki Koike
    • Chengzeng Yin
    • Yuki Sato
    • Yuka Nagano
    • Akira Yamamoto
    • Takahito Kitajima
    • Tadanobu Shimura
    • Mikio Kawamura
    • Kohei Matsushita
    • Yoshinaga Okugawa
    • Keishiro Amano
    • Kohei Otake
    • Yoshiki Okita
    • Masaki Ohi
    • Mikihiro Inoue
    • Keiichi Uchida
    • Masahiro Hirayama
    • Yuji Toiyama
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal and Pediatric Surgery, Division of Reparative Medicine, Institute of Life Sciences, Mie University Graduate School of Medicine, Tsu, Mie 514‑8507, Japan, Department of Pediatrics, Mie University Graduate School of Medicine, Tsu, Mie 514‑8507, Japan, Department of Surgery, Mie Prefectural General Medical Center, Yokkaichi, Mie 510‑0885, Japan
    Copyright: © Koike et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 136
    |
    Published online on: February 28, 2022
       https://doi.org/10.3892/ol.2022.13256
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Abstract

Targeting protein for Xenopus kinesin‑like protein 2 (TPX2) is upregulated in various tumors, and several studies have demonstrated the role of TPX2 as a prognostic marker in cancer. However, the function of TPX2 in neuroblastoma (NB) has not been completely elucidated. In the present study, the clinical significance and functional role of TPX2 in NB was investigated. The Therapeutically Applicable Research to Generate Effective Treatments (TARGET)‑NB dataset was used. A total of 43 patients with NB were enrolled in the present study as the validation set. After evaluating the prognostic role of TPX2, the combined predictive effect of TPX2 and MYCN proto‑oncogene bHLH transcription factor (MYCN) gene amplification was assessed. Double immunofluorescence staining for TPX2 and N‑Myc was used to analyze colocalization, and multiple cell function tests were performed by means of in vitro experiments to elucidate the functional role of TPX2 using RNA interference technology in NB cell lines. In both the TARGET‑NB set and the validation set, it was found that upregulated of TPX2 was significantly associated with poor overall survival (OS) in patients with NB. The expression of TPX2 was higher in NB patients with MYCN gene amplification, and NB patients with high TPX2 expression and MYCN gene amplification had the poorest OS compared with patients with low TPX2 expression or a single copy of MYCN. In vitro experiments indicated that TPX2 positively regulated cell proliferation and the cell cycle, and promoted cell survival by increasing the resistance to apoptosis. The colocalization of TPX2 with N‑Myc in NB cells and tissue was observed. The findings of the present study indicate that TPX2 plays an oncogenic role in NB development and may be a potential prognostic indicator in patients with NB.
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Copy and paste a formatted citation
Spandidos Publications style
Koike Y, Yin C, Sato Y, Nagano Y, Yamamoto A, Kitajima T, Shimura T, Kawamura M, Matsushita K, Okugawa Y, Okugawa Y, et al: TPX2 is a prognostic marker and promotes cell proliferation in neuroblastoma. Oncol Lett 23: 136, 2022.
APA
Koike, Y., Yin, C., Sato, Y., Nagano, Y., Yamamoto, A., Kitajima, T. ... Toiyama, Y. (2022). TPX2 is a prognostic marker and promotes cell proliferation in neuroblastoma. Oncology Letters, 23, 136. https://doi.org/10.3892/ol.2022.13256
MLA
Koike, Y., Yin, C., Sato, Y., Nagano, Y., Yamamoto, A., Kitajima, T., Shimura, T., Kawamura, M., Matsushita, K., Okugawa, Y., Amano, K., Otake, K., Okita, Y., Ohi, M., Inoue, M., Uchida, K., Hirayama, M., Toiyama, Y."TPX2 is a prognostic marker and promotes cell proliferation in neuroblastoma". Oncology Letters 23.4 (2022): 136.
Chicago
Koike, Y., Yin, C., Sato, Y., Nagano, Y., Yamamoto, A., Kitajima, T., Shimura, T., Kawamura, M., Matsushita, K., Okugawa, Y., Amano, K., Otake, K., Okita, Y., Ohi, M., Inoue, M., Uchida, K., Hirayama, M., Toiyama, Y."TPX2 is a prognostic marker and promotes cell proliferation in neuroblastoma". Oncology Letters 23, no. 4 (2022): 136. https://doi.org/10.3892/ol.2022.13256
Copy and paste a formatted citation
x
Spandidos Publications style
Koike Y, Yin C, Sato Y, Nagano Y, Yamamoto A, Kitajima T, Shimura T, Kawamura M, Matsushita K, Okugawa Y, Okugawa Y, et al: TPX2 is a prognostic marker and promotes cell proliferation in neuroblastoma. Oncol Lett 23: 136, 2022.
APA
Koike, Y., Yin, C., Sato, Y., Nagano, Y., Yamamoto, A., Kitajima, T. ... Toiyama, Y. (2022). TPX2 is a prognostic marker and promotes cell proliferation in neuroblastoma. Oncology Letters, 23, 136. https://doi.org/10.3892/ol.2022.13256
MLA
Koike, Y., Yin, C., Sato, Y., Nagano, Y., Yamamoto, A., Kitajima, T., Shimura, T., Kawamura, M., Matsushita, K., Okugawa, Y., Amano, K., Otake, K., Okita, Y., Ohi, M., Inoue, M., Uchida, K., Hirayama, M., Toiyama, Y."TPX2 is a prognostic marker and promotes cell proliferation in neuroblastoma". Oncology Letters 23.4 (2022): 136.
Chicago
Koike, Y., Yin, C., Sato, Y., Nagano, Y., Yamamoto, A., Kitajima, T., Shimura, T., Kawamura, M., Matsushita, K., Okugawa, Y., Amano, K., Otake, K., Okita, Y., Ohi, M., Inoue, M., Uchida, K., Hirayama, M., Toiyama, Y."TPX2 is a prognostic marker and promotes cell proliferation in neuroblastoma". Oncology Letters 23, no. 4 (2022): 136. https://doi.org/10.3892/ol.2022.13256
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