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Article

Glycoprotein hormone α‑subunit promotes cell proliferation and tumorigenesis in breast cancer

  • Authors:
    • Jun Zhou
    • Xuan Zhu
    • Shijie Wu
    • Yiding Chen
  • View Affiliations / Copyright

    Affiliations: Department of Breast Surgery, The Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China, Department of Radiation Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China, Department of Surgical Oncology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, P.R. China
  • Article Number: 142
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    Published online on: March 11, 2022
       https://doi.org/10.3892/ol.2022.13263
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Abstract

The glycoprotein hormone α‑subunit (CGA) is implicated in the occurrence and progression of a number of solid tumors. However, its role in breast cancer remains unclear. The present study aimed to investigate the biological functions and mechanisms of action of CGA in breast cancer. CGA protein expression was evaluated in clinical breast cancer specimens using immunohistochemistry. The association between CGA expression and patient prognosis was determined using the Kaplan‑Meier method and Mantel‑Cox test. At the same time, CGA mRNA and protein expression was explored in a normal mammary epithelial cell line and breast cancer cell lines. Breast cancer cell lines overexpressing or deficient in CGA were established, and the effect of CGA on cell proliferation was evaluated in vitro, and in vivo using a mouse xenograft tumor model. Intracellular signaling pathway activities were evaluated using western blotting in CGA‑overexpressing or ‑depleted cells. Increased CGA protein expression was significantly associated with a poor prognosis in patients with breast cancer. Furthermore, while CGA mRNA and protein expression level was negligible in normal mammary epithelial cells, it was elevated in breast cancer cell lines. In vitro and in vivo experiments showed that CGA overexpression enhanced breast cancer cell proliferation via activation of the epidermal growth factor receptor, extracellular signal‑regulated kinase 1/2 and serine/threonine kinase Akt signaling cascades. The present results suggest that CGA is upregulated in breast cancer tissues and that it is associated with a poor prognosis. CGA may serve as a candidate for developing targeted therapies for breast cancer.
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1 

Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A and Bray F: Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 71:209–249. 2021. View Article : Google Scholar : PubMed/NCBI

2 

Ji P, Gong Y, Jin ML, Hu X, Di GH and Shao ZM: The burden and trends of breast cancer from 1990 to 2017 at the global, regional, and national levels: Results from the global burden of disease study 2017. Front Oncol. 10:6502020. View Article : Google Scholar : PubMed/NCBI

3 

Heer E, Harper A, Escandor N, Sung H, McCormack V and Fidler-Benaoudia MM: Global burden and trends in premenopausal and postmenopausal breast cancer: A population-based study. Lancet Glob Health. 8:e1027–e1037. 2020. View Article : Google Scholar : PubMed/NCBI

4 

Fiddes JC and Goodman HM: The gene encoding the common alpha subunit of the four human glycoprotein hormones. J Mol Appl Genet. 1:3–18. 1981.PubMed/NCBI

5 

Watkins PC, Eddy R, Beck AK, Vellucci V, Leverone B, Tanzi RE, Gusella JF and Shows TB: DNA sequence and regional assignment of the human follicle-stimulating hormone beta-subunit gene to the short arm of human chromosome 11. DNA. 6:205–212. 1987. View Article : Google Scholar : PubMed/NCBI

6 

Rivera RT, Pasion SG, Wong DT, Fei YB and Biswas DK: Loss of tumorigenic potential by human lung tumor cells in the presence of antisense RNA specific to the ectopically synthesized alpha subunit of human chorionic gonadotropin. J Cell Biol. 108:2423–2434. 1989. View Article : Google Scholar : PubMed/NCBI

7 

Bièche I, Latil A, Parfait B, Vidaud D, Laurendeau I, Lidereau R, Cussenot O and Vidaud M: CGA gene (coding for the alpha subunit of glycoprotein hormones) overexpression in ER alpha-positive prostate tumors. Eur Urol. 41:335–341. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Li YL, Yang TS, Ruan WM, Cui W, Jin Y and Zou XM: Effect of trichostatin a on SGC-7901 gastric cancer cells. Int J Clin Exp Med. 7:1958–1966. 2014.PubMed/NCBI

9 

Bieche I, Parfait B, Le Doussal V, Olivi M, Rio MC, Lidereau R and Vidaud M: Identification of CGA as a novel estrogen receptor-responsive gene in breast cancer: An outstanding candidate marker to predict the response to endocrine therapy. Cancer Res. 61:1652–1658. 2001.PubMed/NCBI

10 

Bièche I, Parfait B, Noguès C, Andrieu C, Vidaud D, Spyratos F, Lidereau R and Vidaud M: The CGA gene as new predictor of the response to endocrine therapy in ER alpha-positive postmenopausal breast cancer patients. Oncogene. 20:6955–6959. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Del Mastro L, Ceppi M, Poggio F, Bighin C, Peccatori F, Demeestere I, Levaggi A, Giraudi S, Lambertini M, D'Alonzo A, et al: Gonadotropin-releasing hormone analogues for the prevention of chemotherapy-induced premature ovarian failure in cancer women: Systematic review and meta-analysis of randomized trials. Cancer Treat Rev. 40:675–683. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Blumenfeld Z: Fertility preservation and GnRHa for chemotherapy: Debate. Cancer Manag Res. 6:313–315. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Day EK, Sosale NG and Lazzara MJ: Cell signaling regulation by protein phosphorylation: A multivariate, heterogeneous, and context-dependent process. Curr Opin Biotechnol. 40:185–192. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Walker RA: Significance of alpha-subunit HCG demonstrated in breast carcinomas by the immunoperoxidase technique. J Clin Pathol. 31:245–249. 1978. View Article : Google Scholar : PubMed/NCBI

16 

Zhou J, Chen Y, Huang Y, Long J, Wan F and Zhang S: Serum follicle-stimulating hormone level is associated with human epidermal growth factor receptor type 2 and Ki67 expression in post-menopausal females with breast cancer. Oncol Lett. 6:1128–1132. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Wee P and Wang Z: Epidermal growth factor receptor cell proliferation signaling pathways. Cancers (Basel). 9:522017. View Article : Google Scholar : PubMed/NCBI

18 

Lawlor MA and Alessi DR: PKB/Akt: A key mediator of cell proliferation, survival and insulin responses? J Cell Sci. 114:2903–2910. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Gao J, Aksoy BA, Dogrusoz U, Dresdner G, Gross B, Sumer SO, Sun Y, Jacobsen A, Sinha R, Larsson E, et al: Integrative analysis of complex cancer genomics and clinical profiles using the cBioPortal. Sci Signal. 6:pl12013. View Article : Google Scholar : PubMed/NCBI

20 

Cerami E, Gao J, Dogrusoz U, Gross BE, Sumer SO, Aksoy BA, Jacobsen A, Byrne CJ, Heuer ML, Larsson E, et al: The cBio cancer genomics portal: An open platform for exploring multidimensional cancer genomics data. Cancer Discov. 2:401–404. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Warde-Farley D, Donaldson SL, Comes O, Zuberi K, Badrawi R, Chao P, Franz M, Grouios C, Kazi F, Lopes CT, et al: The GeneMANIA prediction server: biological network integration for gene prioritization and predicting gene function. Nucleic Acids Res. 38:W214–W220. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Xunyi Y, Zhentao Y, Dandan J and Funian L: Clinicopathological significance of PTPN12 expression in human breast cancer. Braz J Med Biol Res. 45:1334–1340. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Espejo R, Rengifo-Cam W, Schaller MD, Evers BM and Sastry SK: PTP-PEST controls motility, adherens junction assembly, and Rho GTPase activity in colon cancer cells. Am J Physiol Cell Physiol. 299:C454–C463. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Sun T, Aceto N, Meerbrey KL, Kessler JD, Zhou C, Migliaccio I, Nguyen DX, Pavlova NN, Botero M, Huang J, et al: Activation of multiple proto-oncogenic tyrosine kinases in breast cancer via loss of the PTPN12 phosphatase. Cell. 144:703–718. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Villa-Moruzzi E: Tyrosine phosphatases in the HER2-directed motility of ovarian cancer cells: Involvement of PTPN12, ERK5 and FAK. Anal Cell Pathol (Amst). 34:101–112. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Villa-Moruzzi E: PTPN12 controls PTEN and the AKT signalling to FAK and HER2 in migrating ovarian cancer cells. Mol Cell Biochem. 375:151–157. 2013.PubMed/NCBI

27 

Singletary SE and Greene FL; Breast Task Force, : Revision of breast cancer staging: The 6th edition of the TNM Classification. Semin Surg Oncol. 21:53–59. 2003. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhou J, Zhu X, Wu S and Chen Y: Glycoprotein hormone α‑subunit promotes cell proliferation and tumorigenesis in breast cancer. Oncol Lett 23: 142, 2022.
APA
Zhou, J., Zhu, X., Wu, S., & Chen, Y. (2022). Glycoprotein hormone α‑subunit promotes cell proliferation and tumorigenesis in breast cancer. Oncology Letters, 23, 142. https://doi.org/10.3892/ol.2022.13263
MLA
Zhou, J., Zhu, X., Wu, S., Chen, Y."Glycoprotein hormone α‑subunit promotes cell proliferation and tumorigenesis in breast cancer". Oncology Letters 23.5 (2022): 142.
Chicago
Zhou, J., Zhu, X., Wu, S., Chen, Y."Glycoprotein hormone α‑subunit promotes cell proliferation and tumorigenesis in breast cancer". Oncology Letters 23, no. 5 (2022): 142. https://doi.org/10.3892/ol.2022.13263
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou J, Zhu X, Wu S and Chen Y: Glycoprotein hormone α‑subunit promotes cell proliferation and tumorigenesis in breast cancer. Oncol Lett 23: 142, 2022.
APA
Zhou, J., Zhu, X., Wu, S., & Chen, Y. (2022). Glycoprotein hormone α‑subunit promotes cell proliferation and tumorigenesis in breast cancer. Oncology Letters, 23, 142. https://doi.org/10.3892/ol.2022.13263
MLA
Zhou, J., Zhu, X., Wu, S., Chen, Y."Glycoprotein hormone α‑subunit promotes cell proliferation and tumorigenesis in breast cancer". Oncology Letters 23.5 (2022): 142.
Chicago
Zhou, J., Zhu, X., Wu, S., Chen, Y."Glycoprotein hormone α‑subunit promotes cell proliferation and tumorigenesis in breast cancer". Oncology Letters 23, no. 5 (2022): 142. https://doi.org/10.3892/ol.2022.13263
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