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MicroRNA‑20a promotes non‑small cell lung cancer proliferation by upregulating PD‑L1 by targeting PTEN

  • Authors:
    • Jiaomei Gong
    • Yong Shen
    • Fuguo Jiang
    • Yan Wang
    • Lulu Chu
    • Jinqi Sun
    • Pengxiao Shen
    • Maocai Chen
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory, Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450013, P.R. China, Department of Clinical Laboratory, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan 450008, P.R. China, Department of Clinical Laboratory, Jiaozuo People's Hospital, Jiaozuo, Henan 454000, P.R. China
    Copyright: © Gong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 148
    |
    Published online on: March 15, 2022
       https://doi.org/10.3892/ol.2022.13269
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Abstract

Non‑small cell lung cancer (NSCLC) remains one of the most common malignant tumors worldwide. The aim of the present study was to investigate the possibility of microRNA‑20a (miR‑20a) as a biomarker and therapeutic target for the diagnosis and treatment of NSCLC. Bioinformatics prediction, together with functional validation, confirmed miR‑20a bound to programmed death ligand‑1 (PD‑L1) 3'‑untranslated region to upregulate PD‑L1 expression. Both miR‑20a and PD‑L1 could promote the proliferation of NSCLC cells. The expression level of PD‑L1 was controlled by PTEN; however, further upstream regulation of PD‑L1 expression was largely unknown. The present study showed that miR‑20a could not restore the inhibition of PD‑L1 expression levels by PTEN. Knockdown of PTEN expression upregulated the expression level of PD‑L1 and promoted the proliferation of NSCLC cells. PTEN negatively regulated the Wnt/β‑catenin signaling pathway by inhibiting β‑catenin and Cyclin D1. Interestingly, PTEN could reverse miR‑20a‑mediated proliferation of NSCLC cells and the inhibitory effect was similar to that of XAV‑939. miR‑20a promotes the proliferation of NSCLC cells by inhibiting the expression level of PTEN and upregulating the expression level of PD‑L1. It is suggested that miR‑20a could be used as a biomarker and therapeutic target for the treatment of NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Gong J, Shen Y, Jiang F, Wang Y, Chu L, Sun J, Shen P and Chen M: MicroRNA‑20a promotes non‑small cell lung cancer proliferation by upregulating <em>PD‑L1</em> by targeting <em>PTEN</em>. Oncol Lett 23: 148, 2022.
APA
Gong, J., Shen, Y., Jiang, F., Wang, Y., Chu, L., Sun, J. ... Chen, M. (2022). MicroRNA‑20a promotes non‑small cell lung cancer proliferation by upregulating <em>PD‑L1</em> by targeting <em>PTEN</em>. Oncology Letters, 23, 148. https://doi.org/10.3892/ol.2022.13269
MLA
Gong, J., Shen, Y., Jiang, F., Wang, Y., Chu, L., Sun, J., Shen, P., Chen, M."MicroRNA‑20a promotes non‑small cell lung cancer proliferation by upregulating <em>PD‑L1</em> by targeting <em>PTEN</em>". Oncology Letters 23.5 (2022): 148.
Chicago
Gong, J., Shen, Y., Jiang, F., Wang, Y., Chu, L., Sun, J., Shen, P., Chen, M."MicroRNA‑20a promotes non‑small cell lung cancer proliferation by upregulating <em>PD‑L1</em> by targeting <em>PTEN</em>". Oncology Letters 23, no. 5 (2022): 148. https://doi.org/10.3892/ol.2022.13269
Copy and paste a formatted citation
x
Spandidos Publications style
Gong J, Shen Y, Jiang F, Wang Y, Chu L, Sun J, Shen P and Chen M: MicroRNA‑20a promotes non‑small cell lung cancer proliferation by upregulating <em>PD‑L1</em> by targeting <em>PTEN</em>. Oncol Lett 23: 148, 2022.
APA
Gong, J., Shen, Y., Jiang, F., Wang, Y., Chu, L., Sun, J. ... Chen, M. (2022). MicroRNA‑20a promotes non‑small cell lung cancer proliferation by upregulating <em>PD‑L1</em> by targeting <em>PTEN</em>. Oncology Letters, 23, 148. https://doi.org/10.3892/ol.2022.13269
MLA
Gong, J., Shen, Y., Jiang, F., Wang, Y., Chu, L., Sun, J., Shen, P., Chen, M."MicroRNA‑20a promotes non‑small cell lung cancer proliferation by upregulating <em>PD‑L1</em> by targeting <em>PTEN</em>". Oncology Letters 23.5 (2022): 148.
Chicago
Gong, J., Shen, Y., Jiang, F., Wang, Y., Chu, L., Sun, J., Shen, P., Chen, M."MicroRNA‑20a promotes non‑small cell lung cancer proliferation by upregulating <em>PD‑L1</em> by targeting <em>PTEN</em>". Oncology Letters 23, no. 5 (2022): 148. https://doi.org/10.3892/ol.2022.13269
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