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Downregulation of FTL decreases proliferation of malignant mesothelioma cells by inducing G1 cell cycle arrest

  • Authors:
    • Takahiro Kambara
    • Vishwa Jeet Amatya
    • Kei Kushitani
    • Yutaro Fujii
    • Ihiro Endo
    • Yukio Takeshima
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Hiroshima 734‑8551, Japan
    Copyright: © Kambara et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 174
    |
    Published online on: April 13, 2022
       https://doi.org/10.3892/ol.2022.13294
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Abstract

Pleural malignant mesothelioma is a malignant tumor with a poor prognosis that is strongly associated with asbestos exposure during its development. Because there is no adequate treatment for malignant mesothelioma, investigation of its molecular mechanism is important. The ferritin light chain (FTL) is a subunit of ferritin, and its high expression in malignant tumors, including malignant mesothelioma, has recently been reported; however, its role in malignant mesothelioma is unclear. The purpose of the present study was to clarify the function of FTL in malignant mesothelioma. The expression levels of FTL in malignant mesothelioma were examined using the Cancer Cell Line Encyclopedia database and our previous data. The short interfering (si)RNA against FTL was transfected into two mesothelioma cell lines, ACC‑MESO‑1 and CRL‑5915, and functional analysis was performed. Expression of p21, p27, cyclin‑dependent kinase 2 (CDK2) and phosphorylated retinoblastoma protein (pRb) associated with the cell cycle were examined as candidate genes associated with FTL. The expression levels of the FTL mRNA were higher in malignant mesothelioma compared with other tumors in the Cancer Cell Line Encyclopedia database, and among other genes in our previous study. Reverse transcription‑quantitative PCR and western blotting demonstrated suppression of FTL expression in two cell lines transfected with FTL siRNA compared with cells transfected with negative control (NC) siRNA. In the two cell lines transfected with FTL siRNA, proliferation was significantly suppressed, and cell cycle arrest was observed in the G1 phase. The levels of p21 and p27 were increased, while those of CDK2 and pRb were decreased compared with NC. However, no significant differences in invasion and migration ability were revealed between FTL siRNA‑transfected cells and NC. In conclusion, FTL may increase the proliferative capacity of malignant mesothelioma cells by affecting p21, p27, CDK2 and pRb, and promoting the cell cycle at the G1 phase.
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Copy and paste a formatted citation
Spandidos Publications style
Kambara T, Amatya VJ, Kushitani K, Fujii Y, Endo I and Takeshima Y: Downregulation of FTL decreases proliferation of malignant mesothelioma cells by inducing G<sub>1</sub> cell cycle arrest. Oncol Lett 23: 174, 2022.
APA
Kambara, T., Amatya, V.J., Kushitani, K., Fujii, Y., Endo, I., & Takeshima, Y. (2022). Downregulation of FTL decreases proliferation of malignant mesothelioma cells by inducing G<sub>1</sub> cell cycle arrest. Oncology Letters, 23, 174. https://doi.org/10.3892/ol.2022.13294
MLA
Kambara, T., Amatya, V. J., Kushitani, K., Fujii, Y., Endo, I., Takeshima, Y."Downregulation of FTL decreases proliferation of malignant mesothelioma cells by inducing G<sub>1</sub> cell cycle arrest". Oncology Letters 23.6 (2022): 174.
Chicago
Kambara, T., Amatya, V. J., Kushitani, K., Fujii, Y., Endo, I., Takeshima, Y."Downregulation of FTL decreases proliferation of malignant mesothelioma cells by inducing G<sub>1</sub> cell cycle arrest". Oncology Letters 23, no. 6 (2022): 174. https://doi.org/10.3892/ol.2022.13294
Copy and paste a formatted citation
x
Spandidos Publications style
Kambara T, Amatya VJ, Kushitani K, Fujii Y, Endo I and Takeshima Y: Downregulation of FTL decreases proliferation of malignant mesothelioma cells by inducing G<sub>1</sub> cell cycle arrest. Oncol Lett 23: 174, 2022.
APA
Kambara, T., Amatya, V.J., Kushitani, K., Fujii, Y., Endo, I., & Takeshima, Y. (2022). Downregulation of FTL decreases proliferation of malignant mesothelioma cells by inducing G<sub>1</sub> cell cycle arrest. Oncology Letters, 23, 174. https://doi.org/10.3892/ol.2022.13294
MLA
Kambara, T., Amatya, V. J., Kushitani, K., Fujii, Y., Endo, I., Takeshima, Y."Downregulation of FTL decreases proliferation of malignant mesothelioma cells by inducing G<sub>1</sub> cell cycle arrest". Oncology Letters 23.6 (2022): 174.
Chicago
Kambara, T., Amatya, V. J., Kushitani, K., Fujii, Y., Endo, I., Takeshima, Y."Downregulation of FTL decreases proliferation of malignant mesothelioma cells by inducing G<sub>1</sub> cell cycle arrest". Oncology Letters 23, no. 6 (2022): 174. https://doi.org/10.3892/ol.2022.13294
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