Open Access

miR‑99a‑5p inhibits glycolysis and induces cell apoptosis in cervical cancer by targeting RRAGD

  • Authors:
    • Gang Wang
    • Yu Lu
    • Shi Di
    • Maohua Xie
    • Fang Jing
    • Xiaoyan Dai
  • View Affiliations

  • Published online on: May 27, 2022     https://doi.org/10.3892/ol.2022.13349
  • Article Number: 228
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cervical cancer (CC) is one of the most common gynecological malignancies that endangers women's health. A negative effect of glycolysis is that it contributes to abnormal tumor growth. MicroRNA (miR)‑99a expression has been found to be decreased in CC. The present study aimed to investigate the role of miR‑99a‑5p in glycolysis in CC. For this purpose, the association between miR‑99a and the prognosis of patients with CC from The Cancer Genome Atlas database was analyzed using Kaplan‑Meier analysis. miR‑99a‑5p expression and Ras‑related GTP binding D (RRAGD) expression were assessed using reverse transcription‑quantitative PCR and western blot analysis. Cell proliferation and apoptosis were examined using an MTT assay and flow cytometry, respectively. Glucose uptake, lactate concentration and extracellular acidification rate were measured using a glucose uptake colorimetric assay, a lactate colorimetric assay and a Seahorse XFe96 extracellular flux analyzer, respectively. The association between miR‑99a‑5p and RRAGD was predicted using TargetScan 7.1, and was confirmed using dual luciferase reporter assay. The results revealed that miR‑99a‑5p expression was decreased and that of RRAGD was increased in CC tissues and cell lines. RRAGD was negatively regulated by miR‑99a‑5p. The overexpression of miR‑99a‑5p induced apoptosis and inhibited glycolysis in CC cells by targeting RRAGD. On the whole, the present study revealed a novel mechanism through which miR‑99a‑5p regulates cell apoptosis and glycolysis in CC, thus providing a potential therapeutic target for CC.

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July-2022
Volume 24 Issue 1

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Copy and paste a formatted citation
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Spandidos Publications style
Wang G, Lu Y, Di S, Xie M, Jing F and Dai X: miR‑99a‑5p inhibits glycolysis and induces cell apoptosis in cervical cancer by targeting RRAGD. Oncol Lett 24: 228, 2022
APA
Wang, G., Lu, Y., Di, S., Xie, M., Jing, F., & Dai, X. (2022). miR‑99a‑5p inhibits glycolysis and induces cell apoptosis in cervical cancer by targeting RRAGD. Oncology Letters, 24, 228. https://doi.org/10.3892/ol.2022.13349
MLA
Wang, G., Lu, Y., Di, S., Xie, M., Jing, F., Dai, X."miR‑99a‑5p inhibits glycolysis and induces cell apoptosis in cervical cancer by targeting RRAGD". Oncology Letters 24.1 (2022): 228.
Chicago
Wang, G., Lu, Y., Di, S., Xie, M., Jing, F., Dai, X."miR‑99a‑5p inhibits glycolysis and induces cell apoptosis in cervical cancer by targeting RRAGD". Oncology Letters 24, no. 1 (2022): 228. https://doi.org/10.3892/ol.2022.13349