Propofol inhibits the malignant development of osteosarcoma U2OS cells via AMPK/FΟΧO1‑mediated autophagy

Dai,Lina; Li,Shimei; Li,Xi; Jiang,Bo

doi:10.3892/ol.2022.13430

Propofol inhibits the malignant development of osteosarcoma U2OS cells via AMPK/FΟΧO1‑mediated autophagy

Authors:
- Lina Dai
- Shimei Li
- Xi Li
- Bo Jiang
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Affiliations: Department of Anesthesiology, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou 550001, P.R. China, Department of Anesthesiology, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou 550001, P.R. China, Department of Orthopedic and Sports Medicine, Ningbo First Hospital, Ningbo, Zhejiang 315000, P.R. China
Published online on: July 15, 2022 https://doi.org/10.3892/ol.2022.13430
Article Number: 310
Copyright: © Dai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

It has previously been reported that propofol regulates the development of human osteosarcoma (OS). However, the specific molecular mechanisms underlying the effect of propofol on OS remain poorly understood. Therefore, the aim of the present study was to explore the effects of propofol on OS U2OS cells and the potential underlying mechanism. The Cell Counting Kit‑8 and colony formation assays were performed to assess cell viability and proliferation. Furthermore, cell apoptosis was assessed using the TUNEL assay and western blotting. Wound healing and Transwell assays were performed to evaluate OS cell migration and invasion abilities, respectively. The protein expression levels of epithelial‑mesenchymal transition (EMT)‑, autophagy‑ and adenosine monophosphate‑activated protein kinase (AMPK)/FOXO1 signaling pathway‑related proteins were also determined using western blotting. The results demonstrated that propofol significantly reduced the viability of OS cells and promoted autophagy in a dose‑dependent manner. Moreover, cell treatment with propofol significantly enhanced the protein expression levels of phosphorylated (p)‑AMPK and FOXO1, while decreasing the protein levels of p‑FOXO1. Furthermore, treatment with propofol significantly suppressed cell viability, migration and invasion abilities and the EMT of OS cells, and potentially promoted cell apoptosis via inducing autophagy via the AMPK/FOXO1 signaling pathway. In summary, the present study indicated that propofol potentially had an inhibitory effect on the development of OS cells via AMPK/FOXO1‑mediated autophagy. These results have therefore provided an experimental basis for further studies into the therapeutic effect of propofol on OS.

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