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Article Open Access

Mismatch repair proteins expression and tumor‑infiltrating T‑cells in colorectal cancer

  • Authors:
    • Takahiro Shigaki
    • Kenji Fujiyoshi
    • Tomoya Sudo
    • Akihiro Kawahara
    • Hiroyuki Nakane
    • Takato Yomoda
    • Sachiko Nagasu
    • Tetsushi Kinugasa
    • Jun Akiba
    • Fumihiko Fujita
    • Yoshito Akagi
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Kurume University School of Medicine, Kurume, Fukuoka 830‑0011, Japan, Department of Pathology, Kurume University School of Medicine, Kurume, Fukuoka 830‑0011, Japan
    Copyright: © Shigaki et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 396
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    Published online on: September 21, 2022
       https://doi.org/10.3892/ol.2022.13516
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Abstract

Microsatellite instability (MSI) and tumor mutational burden (TMB) are indicators of the tumor mutational load, which can lead to immune cell recruitment. By contrast, the number of tumor‑infiltrating T cells (TITs) is indicative of the host immune response to tumor cells. The present study evaluated if the expression of mismatch repair (MMR) proteins can be used as a precise tool to assess immunogenicity in the tumor microenvironment. A total of 73 colorectal cancer cases were enrolled in the present study. MMR protein expression was assessed using four‑antibodies immunohistochemistry (IHC) targeting MLH1, MSH2, MSH6 and PMS2. TIT was assessed through IHC by counting CD3+ and CD8+ cells in tumor. The enrolled cases were classified into four groups according to MMR and TIT status i) Mismatch repair‑proficient (pMMR) and a high number of TITs (pMMR/TIT‑H); ii) pMMR and a low number of TITs (pMMR/TIT‑L); iii) mismatch repair‑deficient (dMMR) and TIT‑H (dMMR/TIT‑H); and iv) dMMR/TIT‑L]. The present study evaluated the clinicopathological characteristics of the four groups, in addition to the difference of TMB. TMB analysis was counted the number of the somatic mutations through multi‑genes panel using next‑generation sequencing. Clinicopathological characteristics, including age, sex, pathological depth of invasion and lymph node metastasis, were not found to be statistically different between dMMR/TIT‑H and dMMR/TIT‑L groups. Tumors among pMMR/TIT‑H group were associated with poorly differentiation compared with those in pMMR/TIT‑L group (P=0.025). The median TMB among the dMMR/TIT‑H group was the highest in four groups but the median TMB was <10 muts/Mb in dMMR/TIT‑L, pMMR/TIT‑H and pMMR/TIT‑L groups, respectively. However, one tumor in the pMMR/TIT‑H group showed high TMB. The present findings suggest that assessing MMR status alone may not be sufficient to precisely evaluate the antitumor immune response in the tumor microenvironment.
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Copy and paste a formatted citation
Spandidos Publications style
Shigaki T, Fujiyoshi K, Sudo T, Kawahara A, Nakane H, Yomoda T, Nagasu S, Kinugasa T, Akiba J, Fujita F, Fujita F, et al: Mismatch repair proteins expression and tumor‑infiltrating T‑cells in colorectal cancer. Oncol Lett 24: 396, 2022.
APA
Shigaki, T., Fujiyoshi, K., Sudo, T., Kawahara, A., Nakane, H., Yomoda, T. ... Akagi, Y. (2022). Mismatch repair proteins expression and tumor‑infiltrating T‑cells in colorectal cancer. Oncology Letters, 24, 396. https://doi.org/10.3892/ol.2022.13516
MLA
Shigaki, T., Fujiyoshi, K., Sudo, T., Kawahara, A., Nakane, H., Yomoda, T., Nagasu, S., Kinugasa, T., Akiba, J., Fujita, F., Akagi, Y."Mismatch repair proteins expression and tumor‑infiltrating T‑cells in colorectal cancer". Oncology Letters 24.5 (2022): 396.
Chicago
Shigaki, T., Fujiyoshi, K., Sudo, T., Kawahara, A., Nakane, H., Yomoda, T., Nagasu, S., Kinugasa, T., Akiba, J., Fujita, F., Akagi, Y."Mismatch repair proteins expression and tumor‑infiltrating T‑cells in colorectal cancer". Oncology Letters 24, no. 5 (2022): 396. https://doi.org/10.3892/ol.2022.13516
Copy and paste a formatted citation
x
Spandidos Publications style
Shigaki T, Fujiyoshi K, Sudo T, Kawahara A, Nakane H, Yomoda T, Nagasu S, Kinugasa T, Akiba J, Fujita F, Fujita F, et al: Mismatch repair proteins expression and tumor‑infiltrating T‑cells in colorectal cancer. Oncol Lett 24: 396, 2022.
APA
Shigaki, T., Fujiyoshi, K., Sudo, T., Kawahara, A., Nakane, H., Yomoda, T. ... Akagi, Y. (2022). Mismatch repair proteins expression and tumor‑infiltrating T‑cells in colorectal cancer. Oncology Letters, 24, 396. https://doi.org/10.3892/ol.2022.13516
MLA
Shigaki, T., Fujiyoshi, K., Sudo, T., Kawahara, A., Nakane, H., Yomoda, T., Nagasu, S., Kinugasa, T., Akiba, J., Fujita, F., Akagi, Y."Mismatch repair proteins expression and tumor‑infiltrating T‑cells in colorectal cancer". Oncology Letters 24.5 (2022): 396.
Chicago
Shigaki, T., Fujiyoshi, K., Sudo, T., Kawahara, A., Nakane, H., Yomoda, T., Nagasu, S., Kinugasa, T., Akiba, J., Fujita, F., Akagi, Y."Mismatch repair proteins expression and tumor‑infiltrating T‑cells in colorectal cancer". Oncology Letters 24, no. 5 (2022): 396. https://doi.org/10.3892/ol.2022.13516
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