Open Access

Farnesyl diphosphate synthase promotes cell proliferation by regulating gene expression and alternative splicing profiles in HeLa cells

  • Authors:
    • Lijuan Wang
    • Zhigang Chen
    • Dong Chen
    • Bo Kan
    • Yangfang He
    • Hanqing Cai
  • View Affiliations

  • Published online on: February 28, 2023     https://doi.org/10.3892/ol.2023.13731
  • Article Number: 145
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Farnesyl diphosphate synthase (FDPS), an essential enzyme involved in the mevalonate pathway, is implicated in various diseases, including multiple types of cancer. As an RNA‑binding protein (RBP), FDPS is also involved in transcriptional and post‑transcriptional regulation. However, to the best of our knowledge, transcriptome‑wide targets of FDPS still remain unknown. In the present study, FDPS expression patterns in pan‑cancer were analyzed. In addition, it was investigated how FDPS overexpression (FDPS‑OE) regulates the transcriptome in HeLa cells. FDPS‑OE increased the proliferation rate in HeLa cells by MTT assay. Using transcriptome‑wide high throughput sequencing and bioinformatics analysis, it was found that FDPS upregulated the expression levels of genes enriched in cell proliferation and extracellular matrix organization, including the laminin subunit γ2, interferon‑induced proteins with tetratricopeptide repeats 2 and matrix metallopeptidase 19 genes. According to alternative splicing (AS) analysis, FDPS modulated the splicing patterns of the bone morphogenic protein 1, semaphorin 4D, annexin A2 and sirtuin 2 genes, which are enriched in the cell cycle and DNA repair, and are related to cell proliferation. To corroborate the FDPS‑regulated transcriptome findings, FDPS was overexpressed in human osteosarcoma cells. Differentially expressed genes and regulated AS genes in the cells were both validated by reverse transcription‑quantitative PCR. The results suggested that, as an emerging RBP, FDPS may serve an important role in transcriptome profiles by altering gene expression and regulating AS. FDPS also affected the cell proliferation rate. These findings broaden the understanding of the molecular functions of FDPS, and the potential of FDPS as a target in therapy should be investigated.
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April-2023
Volume 25 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wang L, Chen Z, Chen D, Kan B, He Y and Cai H: Farnesyl diphosphate synthase promotes cell proliferation by regulating gene expression and alternative splicing profiles in HeLa cells. Oncol Lett 25: 145, 2023
APA
Wang, L., Chen, Z., Chen, D., Kan, B., He, Y., & Cai, H. (2023). Farnesyl diphosphate synthase promotes cell proliferation by regulating gene expression and alternative splicing profiles in HeLa cells. Oncology Letters, 25, 145. https://doi.org/10.3892/ol.2023.13731
MLA
Wang, L., Chen, Z., Chen, D., Kan, B., He, Y., Cai, H."Farnesyl diphosphate synthase promotes cell proliferation by regulating gene expression and alternative splicing profiles in HeLa cells". Oncology Letters 25.4 (2023): 145.
Chicago
Wang, L., Chen, Z., Chen, D., Kan, B., He, Y., Cai, H."Farnesyl diphosphate synthase promotes cell proliferation by regulating gene expression and alternative splicing profiles in HeLa cells". Oncology Letters 25, no. 4 (2023): 145. https://doi.org/10.3892/ol.2023.13731