Open Access

LPS combined with CD47mAb enhances the anti‑osteosarcoma ability of macrophages

  • Authors:
    • Peng Yuan
    • Yukang Que
    • Yulei Liu
    • Peng He
    • Zehao Guo
    • Yong Hu
  • View Affiliations

  • Published online on: March 28, 2023     https://doi.org/10.3892/ol.2023.13777
  • Article Number: 190
  • Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Macrophages are abundant in tumor tissues, and they affect the biological properties of tumor cells. The present findings indicated that osteosarcoma (OS) has a high proportion of tumor‑promoting M2 macrophages. The CD47 protein can aid tumor cells in their immunological escape. It was identified that CD47 protein is abundant in both clinical OS tissues and OS cell lines. Lipopolysaccharide (LPS) is an activator of Toll‑like receptor 4 present on the surface of macrophages, and it induces the polarization towards a pro‑inflammatory phenotype; and macrophages of pro‑inflammatory phenotype may present antitumor potential. CD47 monoclonal antibody (CD47mAb) can block the CD47‑SIRPα signaling pathway, thus enhancing the antitumor ability of macrophages. Immunofluorescence staining confirmed that OS was rich in CD47 protein and M2 macrophages. In the present study, the antitumor potential of macrophages activated using LPS combined with the CD47mAb was assessed. LPS combined with CD47mAb greatly improved macrophages' capacity to phagocytize OS cells, according to the laser confocal experiments and flow cytometry. Furthermore, cell proliferation analysis, cell migration assay and apoptosis determination confirmed LPS‑polarized macrophages might efficiently suppress OS cells growth and migration while promoting apoptosis. Taken together, the results of present study demonstrated that LPS combined with CD47mAb enhanced the anti‑osteosarcoma ability of macrophages.
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May-2023
Volume 25 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Yuan P, Que Y, Liu Y, He P, Guo Z and Hu Y: LPS combined with CD47mAb enhances the anti‑osteosarcoma ability of macrophages. Oncol Lett 25: 190, 2023
APA
Yuan, P., Que, Y., Liu, Y., He, P., Guo, Z., & Hu, Y. (2023). LPS combined with CD47mAb enhances the anti‑osteosarcoma ability of macrophages. Oncology Letters, 25, 190. https://doi.org/10.3892/ol.2023.13777
MLA
Yuan, P., Que, Y., Liu, Y., He, P., Guo, Z., Hu, Y."LPS combined with CD47mAb enhances the anti‑osteosarcoma ability of macrophages". Oncology Letters 25.5 (2023): 190.
Chicago
Yuan, P., Que, Y., Liu, Y., He, P., Guo, Z., Hu, Y."LPS combined with CD47mAb enhances the anti‑osteosarcoma ability of macrophages". Oncology Letters 25, no. 5 (2023): 190. https://doi.org/10.3892/ol.2023.13777