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Article Open Access

CD19/CD22 bispecific chimeric antigen receptor‑NK‑92 cells are developed and evaluated

  • Authors:
    • Hyori Kim
    • Mina Han
    • Minsong Kim
    • Hyeri Kim
    • Ho Joon Im
    • Nayoung Kim
    • Kyung-Nam Koh
  • View Affiliations / Copyright

    Affiliations: Department of Convergence Medicine and Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea, Division of Pediatric Hematology/Oncology, Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 236
    |
    Published online on: April 19, 2023
       https://doi.org/10.3892/ol.2023.13822
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Abstract

Anti‑CD19 chimeric antigen receptor (CAR)‑T cells have improved the outcomes of patients with B cell leukemia and lymphoma. However, their applications and positive outcomes remain limited. CAR‑T cells are currently restricted to autologous blood as their source and their use can lead to downregulation of CD19 expression along with complications such as graft‑versus‑host disease and cytokine release syndrome. The present study aimed to develop anti‑CD19/CD22 bispecific CAR structures using an anti‑CD22 monoclonal antibody clone from chickens and analyze them in natural killer (NK)‑92 cells, a human NK cell line, in vitro and in vivo. Anti‑CD19/CD22 CAR‑NK‑92 cell cytotoxicity was assessed by the survival of target cells and counted using flow cytometry. Anti‑CD22/CD19 and loop‑structured anti‑CD19/CD22 bi‑specific CAR‑NK‑92 cells showed improved efficacy against OCI‑Ly7 cells, a human B cell lymphoma cell line, compared with other CAR structures. These results demonstrate the potential of anti‑CD19/CD22 bispecific CAR‑NK cells and suggested that optimizing CAR structures in NK cells can improve the efficacy of CAR therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Kim H, Han M, Kim M, Kim H, Im H, Kim N and Koh K: CD19/CD22 bispecific chimeric antigen receptor‑NK‑92 cells are developed and evaluated. Oncol Lett 25: 236, 2023.
APA
Kim, H., Han, M., Kim, M., Kim, H., Im, H., Kim, N., & Koh, K. (2023). CD19/CD22 bispecific chimeric antigen receptor‑NK‑92 cells are developed and evaluated. Oncology Letters, 25, 236. https://doi.org/10.3892/ol.2023.13822
MLA
Kim, H., Han, M., Kim, M., Kim, H., Im, H., Kim, N., Koh, K."CD19/CD22 bispecific chimeric antigen receptor‑NK‑92 cells are developed and evaluated". Oncology Letters 25.6 (2023): 236.
Chicago
Kim, H., Han, M., Kim, M., Kim, H., Im, H., Kim, N., Koh, K."CD19/CD22 bispecific chimeric antigen receptor‑NK‑92 cells are developed and evaluated". Oncology Letters 25, no. 6 (2023): 236. https://doi.org/10.3892/ol.2023.13822
Copy and paste a formatted citation
x
Spandidos Publications style
Kim H, Han M, Kim M, Kim H, Im H, Kim N and Koh K: CD19/CD22 bispecific chimeric antigen receptor‑NK‑92 cells are developed and evaluated. Oncol Lett 25: 236, 2023.
APA
Kim, H., Han, M., Kim, M., Kim, H., Im, H., Kim, N., & Koh, K. (2023). CD19/CD22 bispecific chimeric antigen receptor‑NK‑92 cells are developed and evaluated. Oncology Letters, 25, 236. https://doi.org/10.3892/ol.2023.13822
MLA
Kim, H., Han, M., Kim, M., Kim, H., Im, H., Kim, N., Koh, K."CD19/CD22 bispecific chimeric antigen receptor‑NK‑92 cells are developed and evaluated". Oncology Letters 25.6 (2023): 236.
Chicago
Kim, H., Han, M., Kim, M., Kim, H., Im, H., Kim, N., Koh, K."CD19/CD22 bispecific chimeric antigen receptor‑NK‑92 cells are developed and evaluated". Oncology Letters 25, no. 6 (2023): 236. https://doi.org/10.3892/ol.2023.13822
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