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Article Open Access

The role of miRNA in colorectal cancer diagnosis: A pilot study

  • Authors:
    • Maša Ždralević
    • Janja Raonić
    • Natasa Popovic
    • Ljiljana Vučković
    • Isidora Rovčanin Dragović
    • Batrić Vukčević
    • Vladimir Todorović
    • Filip Vukmirović
    • Flaviana Marzano
    • Apollonia Tullo
    • Nicoletta Guaragnella
    • Sergio Giannattasio
    • Miodrag Radunović
  • View Affiliations / Copyright

    Affiliations: Faculty of Medicine, University of Montenegro, 81000 Podgorica, Montenegro, Institute for Biomembranes, Bioenergetics and Molecular Biotechnologies, National Research Council, I-70126 Bari, Italy, Department of Biosciences, Biotechnologies and Environment, University of Bari ‘Aldo Moro’, I-70126 Bari, Italy
    Copyright: © Ždralević et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 267
    |
    Published online on: May 4, 2023
       https://doi.org/10.3892/ol.2023.13853
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Abstract

Despite recent advances in diagnosis and treatment, colorectal cancer (CRC) remains the third most common cancer worldwide, and has both a poor prognosis and a high recurrence rate, thus indicating the need for new, sensitive and specific biomarkers. MicroRNAs (miRNAs/miRs) are important regulators of gene expression, which are involved in numerous biological processes implicated in tumorigenesis. The objective of the present study was to investigate the expression of miRNAs in plasma and tissue samples from patients with CRC, and to examine their potential as CRC biomarkers. Using reverse transcription‑quantitative PCR, it was revealed that miR‑29a, miR‑101, miR‑125b, miR‑146a and miR‑155 were dysregulated in the formalin‑fixed paraffin‑embedded tissues of patients with CRC, compared with the surrounding healthy tissue, and these miRNAs were associated with several pathological features of the tumor. Bioinformatics analysis of overlapping target genes identified AGE‑RAGE signaling as a putative joint regulatory pathway. miR‑146a was also upregulated in the plasma of patients with CRC, compared with the healthy control group, and had a fair discriminatory power (area under the curve, 0.7006), with 66.7% sensitivity and 77.8% specificity. To the best of our knowledge, this distinct five‑miRNA deregulation pattern in tumor tissue, and upregulation of plasma miR‑146a, were shown for the first time in patients with CRC; however, studies on larger patient cohorts are warranted to confirm their potential to be used as CRC diagnostic biomarkers.
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Copy and paste a formatted citation
Spandidos Publications style
Ždralević M, Raonić J, Popovic N, Vučković L, Rovčanin Dragović I, Vukčević B, Todorović V, Vukmirović F, Marzano F, Tullo A, Tullo A, et al: The role of miRNA in colorectal cancer diagnosis: A pilot study. Oncol Lett 25: 267, 2023.
APA
Ždralević, M., Raonić, J., Popovic, N., Vučković, L., Rovčanin Dragović, I., Vukčević, B. ... Radunović, M. (2023). The role of miRNA in colorectal cancer diagnosis: A pilot study. Oncology Letters, 25, 267. https://doi.org/10.3892/ol.2023.13853
MLA
Ždralević, M., Raonić, J., Popovic, N., Vučković, L., Rovčanin Dragović, I., Vukčević, B., Todorović, V., Vukmirović, F., Marzano, F., Tullo, A., Guaragnella, N., Giannattasio, S., Radunović, M."The role of miRNA in colorectal cancer diagnosis: A pilot study". Oncology Letters 25.6 (2023): 267.
Chicago
Ždralević, M., Raonić, J., Popovic, N., Vučković, L., Rovčanin Dragović, I., Vukčević, B., Todorović, V., Vukmirović, F., Marzano, F., Tullo, A., Guaragnella, N., Giannattasio, S., Radunović, M."The role of miRNA in colorectal cancer diagnosis: A pilot study". Oncology Letters 25, no. 6 (2023): 267. https://doi.org/10.3892/ol.2023.13853
Copy and paste a formatted citation
x
Spandidos Publications style
Ždralević M, Raonić J, Popovic N, Vučković L, Rovčanin Dragović I, Vukčević B, Todorović V, Vukmirović F, Marzano F, Tullo A, Tullo A, et al: The role of miRNA in colorectal cancer diagnosis: A pilot study. Oncol Lett 25: 267, 2023.
APA
Ždralević, M., Raonić, J., Popovic, N., Vučković, L., Rovčanin Dragović, I., Vukčević, B. ... Radunović, M. (2023). The role of miRNA in colorectal cancer diagnosis: A pilot study. Oncology Letters, 25, 267. https://doi.org/10.3892/ol.2023.13853
MLA
Ždralević, M., Raonić, J., Popovic, N., Vučković, L., Rovčanin Dragović, I., Vukčević, B., Todorović, V., Vukmirović, F., Marzano, F., Tullo, A., Guaragnella, N., Giannattasio, S., Radunović, M."The role of miRNA in colorectal cancer diagnosis: A pilot study". Oncology Letters 25.6 (2023): 267.
Chicago
Ždralević, M., Raonić, J., Popovic, N., Vučković, L., Rovčanin Dragović, I., Vukčević, B., Todorović, V., Vukmirović, F., Marzano, F., Tullo, A., Guaragnella, N., Giannattasio, S., Radunović, M."The role of miRNA in colorectal cancer diagnosis: A pilot study". Oncology Letters 25, no. 6 (2023): 267. https://doi.org/10.3892/ol.2023.13853
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