Advances in transcriptomics and proteomics in differentiated thyroid cancer: An updated perspective (Review)
- Authors:
- Published online on: July 27, 2023 https://doi.org/10.3892/ol.2023.13982
- Article Number: 396
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Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Thyroid cancer (TC) is among the most prevalent endocrine malignancies globally, with epidemiological data indicating a 20% annual increase in incidence and a 1.1% annual mortality rate (1–3). This trend is primarily due to the rising occurrence and heightened mortality of papillary thyroid carcinoma (PTC), which, together with follicular thyroid carcinoma (FTC), comprises differentiated thyroid cancer (DTC), accounting for >95% of all TC cases (4,5). The completion of the Human Genome Project in 2003 ushered in the post-genomic era, characterized by the emergence of functional genomics, which seeks to uncover the functions and regulatory mechanisms of the genome (6). Following the advent of the post-genomic era, medical research transitioned into the precision medicine era, leveraging various-omics technologies to elucidate the molecular mechanisms underpinning human malignancies in numerous publications (7,8). DTC is fundamentally a genetic disease, with its development being protracted, multifaceted, staged and intricate, inextricably connected to multiple molecular and environmental factors (5,8). Transcriptomics and proteomics involve the comprehensive study of gene transcription or proteins in living organisms. The vast amount of data generated by high-throughput transcriptome sequencing enables the identification of gene-specific characteristics by analyzing genes correlated with phenotypic information. By contrast, proteomics, as a functional translation of the genome, offers direct insight into the mechanisms that instigate pathogenic effects at the genomic level (9). Consequently, transcriptomics and proteomics can be employed to identify molecular markers of DTC, monitor its progression and select and validate therapeutic targets, demonstrating their long-term potential in clinical diagnostics and treatment. The present review examined the crucial transcriptomic and proteomic data of DTC identified recently, offering novel perspectives for the advancement of DTC diagnosis and therapy.
Transcriptome analysis of DTC
Recent advancements in transcriptomics-based sequencing analysis have led to the identification of potential molecular markers for DTC at various levels, such as pathogenic risk (10,11), early diagnosis (12–14), metastasis and progression (15–19), prognosis prediction (20–25) and therapeutic targets (26–32) (Table I). This progress has increased our understanding of the molecular mechanisms underlying DTC and has helped resolve complex issues. However, only a few of these molecules have proved useful in clinical settings. The present review focused on the molecular classification of DTC and radioactive iodine (131I) refractory (RAIR)-DTC, which is DTC that does not respond to radioactive iodine.
Table I.Application of massively parallel sequencing methods to analyze the latest partial transcriptome studies of DTC. |
Molecular subtypes
DTC is categorized into distinct subtypes according to their gene expression levels, revealing differences in clinicopathological features, intracellular mechanisms and mutational profiles that contribute to a deeper understanding of TC. The initial integrative study by the Cancer Genome Atlas (TCGA) in 2014 (33) classified PTC into two molecular subtypes: BRAF-like (BVL) and RAS-like (RL), which are associated with BRAF or RAS mutations, respectively. BVL exhibits more aggressive clinical features compared with RL. This research marked the first transcriptome-based classification of PTC molecular subtypes. The study employed unsupervised clustering methods on four genomic datasets, each producing distinct subtypes that supported the overarching separation of BVL-PTC and RL-PTC. Patients in the RL-PTC group exhibited follicular variant histology, a relatively low recurrence risk and highly differentiated tumors associated with younger patients. By contrast, the different datasets divided the BVL-PTC group into varying numbers of subtypes that did not overlap, resulting in an inconsistent lower-level partitioning of BVL-PTC. To investigate signaling and differentiation in PTC, researchers developed a BRAFV600E-RAS score (BRS) and a thyroid differentiation score (TDS). The BRS quantifies the extent to which the gene expression profile of a given tumor resembles either the BRAFV600E- or RAS-mutant profiles, providing a continuous reference scale from most BVL to most RL to examine the signaling consequences of other, less common mutations. The TDS, derived from the expression levels of 16 highly correlated thyroid metabolic and functional genes within this cohort, plays a central role in TC. Based on the TDS and BRS results, the study (30) found that all BRAF fusions were BVL. Among the six EIF1AX mutations, four were RL, one was neutral and one was weakly BVL. All PAX8/peroxisome proliferator activated receptor γ (PPARG) fusions were RL, in line with their prevalence in follicular-patterned tumors. Almost all Ret Proto-Oncogene (RET) fusions were weakly BVL, while non-invasive follicular thyroid neoplasm (NTRK)1/3 and anaplastic lymphoma receptor tyrosine kinase (ALK) fusions were predominantly neutral. In conclusion, the comprehensive multi-platform molecular data and large sample size in this study offer an opportunity to refine the classification of PTC into molecular subtypes and correlate them with clinically relevant parameters. The BRS and TDS measures were utilized to elucidate the relationships between tumor cluster, histology, genotype, signaling and differentiation.
A number of studies have supported the notion that there are three molecular subtypes of DTC: BVL, RL and non-BRAF-non-RAS (NBNR) (34,35). Yoo et al (34) conducted a study on follicular thyroid tumors, including CV-PTC, FV-PTC, minimally invasive FTC and FA, using molecular typing by TCGA. In addition to expanding the RL subtype, they analyzed and proposed another molecular subtype, NBNR. The BVL subtype consisted of BRAFV600E and fusion genes, including phosphatidylinositol binding clathrin assembly protein-BRAF, nuclear transcription factor y subunit α-BRAF, staphylococcal nuclease and tudor domain containing 1-BRAF, fibroblast growth factor receptor (FGFR) 2-tryptophanyl-tRNA synthetase, ETS variant transcription factor 6 (ETV6)-NTRK3, sequestosome 1-NTRK1, coiled-coil domain containing (CCDC)6-RET, nuclear receptor coactivator 4-RET and ring finger protein 213-solute carrier family (SLC) 26 member 11. The RL subtype consisted of H/K/NRAS and fusion genes, including Striatin-ALK, Ezrin-Erb-B2 receptor tyrosine kinase 4, fibroblast growth factor receptor 2-KIAA1598, ETV6-NTRK3 and CCDC6-RET. The NBNR subtype was associated with dicer 1, ribonuclease III, EIF1AX, isocitrate dehydrogenase [NADP(+)] 1, phosphatase and tensin homolog, PAX8-PPARG and other driver gene candidates. The study (32) also implemented TDS and ERK scores to explore tumor signaling and differentiation and clinical risk factors were integrated to obtain a progression model of TC. A similar perspective was obtained in a study by Song et al (36). Mutations in SPOP P94R and EZH1 Q571R in nonmalignant thyroid nodules were associated with BVL transcriptomic characteristics, further supporting the proposal of a third molecular subtype (12). Due to its inert biological behavior and molecular profile, enveloped PTC has been reclassified as non-invasive follicular thyroid neoplasm (NIFTP), which exhibits papillary nuclear characteristics (36–39). This reclassification demonstrates that stratification medicine can be achieved by reclassifying TC based on its mutational and transcriptional characteristics, regardless of its histological classification.
Yoo et al (40) proposed an updated view on the progression of DTC to advanced DTC and degenerative interstitial types using previous approaches for anaplastic thyroid cancer (ATC), poorly differentiated thyroid carcinoma, widely invasive FTC and metastatic PTC. The results showed that TERT, AKT1, PIK3CA and EIF1AX were frequently co-mutated with driver genes (BRAFV600E and RAS) in advanced DTCs similar to ATC, while tumor suppressors (e.g., TP53 and CDKN2A) were predominantly altered in ATC. CDKN2A loss was significantly associated with poor disease-specific survival in patients with ATC or advanced DTC and upregulation of programmed cell death 1 ligand (PDCD1LG) 1 and PDCD1LG 2. This led to the proposal of a fourth molecular subtype of TC, ATC-like (AL) and paved the way for targeted therapy of highly invasive and fatal TCs.
Since the 2014 publication of TCGA research on PTC (33), genomic studies of DTC using more advanced massively parallel sequencing have suggested that diverse subtypes involve different mutations, which coupled with their clinical and molecular features, show progressive activation from NBNR to RL to BVL and then to AL, leading to a series of more aggressive behaviors (8,14,33,34,40) (Fig. 1). The molecular subtypes of DTC correspond to different cell signaling and differentiation characteristics, allowing for more accurate pathological classification and diagnosis. However, only a few studies have investigated this topic and further large-scale validation is necessary.
RAIR-DTC
RAIR-DTC constitutes ~1/3 of distant DTC metastases, which are the primary cause of TC mortality, with an average survival of 3–5 years and a 10-year survival rate of ~10% (41). However, molecular-level investigations on RAIR-DTC have been scarce due to the complexity and heterogeneity of RAIR-DTC development, the instability and irreproducibility of the methods employed and the challenges in acquiring clinical samples (42–44). Capdevila et al (45) questioned the limitations of DNA mutation profiles in stratifying prognostic factors. They correlated RNA expression patterns and patient survival in cases enrolled in the DECISION trial, discovering that although RNA expression patterns were associated with DNA mutation patterns and tumor histology, they exhibited considerable differences in multivariate analysis, correlating with prognosis while the other two factors did not. Notably, the RNA expression profiles of BVL and RL revealed 7–9% of BRAF or RAS mutated tumors with ‘unexpected’ expression profiles, suggesting that these classical driver mutations did not dictate the final RNA gene expression profile in these instances. Additionally, ~40% of cases in BVL and RL expression profiles lacked mutations in BRAF or RAS genes, necessitating the identification of potential genomic/epigenomic aberrations that might influence the final expression profile. These findings imply that prognostic biomarkers associated with DTC may become prevalent in individuals with RAIR status. Therefore, it is essential to identify genomic/epigenomic mutations that could potentially affect the eventual expression patterns, as a genome-only stratified prognostic analysis of RAIR-DTC is insufficient. This study (45) also has a limitation due to the use of formalin-fixed and paraffin-embedded (FFPE) samples, which, although representing most tumor sample materials, lack information such as TERT promoter mutations in the prognostic genome.
Another study supports the view of the RAI refractory state. Colombo et al (46) investigated gene/miRNA and molecular expression profiles of PTC primary foci, synchronous lymph node metastases (LNM) and RAI-LNM, noting that patient characteristics with RAI+/D+ (metastatic site with initial RAI uptake and disease persistence) and RAI-/D+ (metastatic site without RAI uptake and disease persistence) still possessed driving lesions (particularly BRAFV600E) of BRAF-/RAS-like subtypes with TDS. These results suggest that DTC maintains its classification as a molecular subtype based on transcriptome expression profiles, even in an RAIR environment. Furthermore, the study found transcriptome similarity in BRAFV600E mutation samples from primary and post-RAI LNM, indicating that RAI treatment has minimal influence on the expression profile of RAIR LNMs compared with primary tumors. This observation may offer a fresh perspective on the challenge of sample accessibility in future RAIR-DTC research.
A recent study utilizing whole-exome sequencing of matched sixty-six iodine-refractory and ninety-two iodine uptake PTC samples identified the APOBEC SBS13 mutation nomogram as an independent predictor of radioiodine resistance in PTC (47). When combined with SBS13 and TERTp mutations, this nomogram significantly increases the likelihood of prediction.
In contrast to adult PTC, molecular characterization of pediatric PTC is crucial for the development of molecule-targeted therapies for progressive RAIR PTC. A genome-wide and transcriptomic study comparing 106 RAIR-DTC children with adults (48) revealed a significant tumor response with the restoration of radioactive iodine uptake when exposed to NTRK and RET fusion-targeted drug therapy. The study also identified age-related drivers of pediatric PTC, thus, supporting fusion-targeted therapy for RAIR-DTC re-differentiation.
Research on RAIR-DTC is currently limited and more molecular studies are necessary to address this bottleneck as quickly as possible.
Proteome analysis of DTC
Recent technological advancements in molecular research have enabled scientists to analyze protein information in various specimens, such as cells, tissues, cell lines and body fluids, using different mass spectrometry techniques, thereby increasing proteome coverage. This has led to the replacement of 2D gel electrophoresis by mass spectrometry coupled with other techniques. DTC proteomics research has made significant strides in improving the accuracy of fine needle aspiration biopsy in diagnosing indeterminate thyroid nodule cases before surgery, as well as in predicting diagnosis and prognosis by identifying biomarkers (Fig. 2; Table II). These advances have facilitated a better understanding of the pathogenic molecular mechanisms associated with the occurrence and progression of DTC, thereby aiding in guiding treatment decisions' (49).
Table II.Application of high-throughput sequencing methods to analyze the latest partial proteomics of DTC. |
Diagnosis of auxiliary fine-needle aspiration biopsy (FNAB) and cytopathology
The study of biomarkers in DTC tissues poses a challenge due to the heterogeneous cellular components that can mask the aberrant expression of tumor proteins. Mass spectrometry imaging (MSI) can resolve certain limitations by accurately and locally determining protein levels within tissues (50), which is vital in classifying indeterminate lesions that cannot be correctly identified by FNAB and cytopathological examination.
Ucal et al (51) used MSI-based label-free quantitative methods to investigate the roles of IQGAP proteins, actin cytoskeletal proteins and energy metabolism alterations in follicular and classical variants of PTC. Another study (52) evaluated the discriminatory ability of matrix-assisted laser desorption/ionization (MALDI) combined with MSI to classify classical variants of PTC using FFPE samples from CV-PTC, FV-PTC and NIFTP. The signal intensities of S100-A6, cytoplasmic actin 1 and vimentin were enhanced within FV-PTC, while 60S ribosomal protein L6/L8 and Prelamin A/C were enhanced in NIFTP and CV-PTC, respectively. The results revealed that the peptide profiles of NIFTP showed significant differences compared with invasive FV-PTC, supporting the renaming of non-invasive FV-PTC to ‘non-invasive follicular thyroid neoplasms with papillary nuclear features’ (NIFTP).
A study using liquid chromatography-tandem mass spectrometry to explore novel protein biomarkers found that upregulated mitochondrial proteins, particularly SUVLG2, differentiate follicular carcinoma (FC) from follicular adenoma (FA) with a sensitivity and specificity of 75 and 80% respectively (53). Furthermore, using mass spectrometry, molecular similarities were observed between seven thyroid specimens, including the major types of thyroid malignancies (FV-PTC, CV-PTC, FTC, ATC, medullary thyroid carcinoma and FA, as well as non-carcinoma thyroid tissue (54,55), demonstrating the high feasibility of MSI combined with various techniques in testing cancer types.
Serum samples offer a readily available, easily monitored and less invasive means of detecting biomarkers for pathological diagnosis in DTC (56). Zhang et al (57) used label-free analysis to identify and quantify the overall proteome, complete N-glycopeptides and desialic acid N-glycopeptides within urine and plasma samples of females with PTC, thus, establishing an integrated, highly reproducible and rapid approach for exploring potential non-invasive diagnostic and prognostic biomarkers and therapeutic targets for PTC. Wang et al (58) discovered that serum-based proteomic profiles showed significantly lower levels of CA4 and plasminogen (PLG) in the PTC group compared with the nodular goiter group. C4A and PLG were identified as excellent diagnostic biomarkers for PTC cases, with sensitivities of 91.67 and 87.50% and specificities of 83.33 and 75.00%, respectively. Another study combining the expression of oncogene NRASQ61R within Nthy-ori 3–1 cells (based on blood and cell lines) with a multi-dimensional proteomics technology DISER, the combination of 2D-difference gel electrophoresis (2D-DIGE) and serological proteome analysis (SERPA), established a comprehensive way to identify disease-related tumor-associated antigens (TAAs) in thyroid tumors, enabling the identification of both cell-based and TAA biomarkers. Among these biomarkers, autoantibodies against CNN3 and PGK1 were identified as tumor-specific biomarkers of the thyroid envelope follicular type/RAS-like phenotype (EFP/RLP), which can be used to differentiate tumors of diverse malignancy grades (59).
MALDI-MSI has shown promise in cytopathology for diagnosing thyroid nodules, using FNAB needle washes as a sample source (60). Furthermore, new diagnostic tools, such as nanoparticle-assisted proteomics approaches, are constantly being developed (61). Taken together, proteomics appears to be a very promising alternative for diagnosing thyroid nodules that are difficult for cytopathologists to identify.
Identification of biomarkers to improve diagnostic or prognostic accuracy
The progression of DTC is marked by invasion and metastasis events, particularly LNM in PTC, which heighten the risk of relapse and result in a dismal prognosis. The discovery of novel protein biomarkers for improved prediction of DTC prognostic risk is of utmost importance.
Dai et al (62) employed TMT-based mass spectrometry and Gene Expression Omnibus and TCGA databases to identify differential proteins between PTC and invasive PTC phenotype (iPTC). They discovered that SLC 27 Member 6, a protein acting as a transporter mediating long-chain fatty acid uptake, was specifically upregulated in iPTC and may be significantly linked to PTC metastasis and staging. Similarly, LAMC2 and myosin IG were found to serve a role in PTC lymph node metastasis and, more importantly, their increased mRNA expression in tumor tissues demonstrated a positive correlation with unfavorable variables such as larger tumor size, LNM, high American Joint Committee on Cancer (AJCC) staging, BRAFV600E mutation and poor prognosis (63). Another study first revealed that PDZ and LIM domain 5 is highly expressed in PTC and could promote the migration, invasion and proliferation of PTC cells through the Ras-ERK pathway (64). Junctional adhesion molecule A, a transmembrane protein involved in various biological processes, including epithelial-to-mesenchymal transition, was shown to have its silencing enhance the proliferation and motility of TC cells, which is closely associated with the phosphorylation of P53 and GSK3α/β proteins. Each of these proteins could positively affect their potential as candidate biomarkers for predicting PTC prognosis (65). Luo et al (66) compared the proteomic profiles of serum purified exosomes (SPEs) of PTC cases (with and without LNM) to those of healthy individuals and observed overexpression of proteins like talin 1, SRC proto-oncogene, non-receptor tyrosine kinase, calpain small subunit 1 and integrin subunit β 2, which are associated with tumor cell migration. Furthermore, abnormal activation of integrin signaling in the SPE of PTC with LNM promoted the aggressiveness of BHT101 TC cells. These findings offer insight into the biological activities and features of serum exosomes during LNM of PTC; thus, aiding in understanding DTC-related molecular mechanisms.
The global increase in PTC morbidity is primarily associated with a rise in papillary thyroid microcarcinoma (PTMC) diagnosis. The need for surgical treatment of PTMC largely depends on LNM. Jin et al (67) studied PTMC with and without LNM and screened for proteins potentially associated with migration and invasion, such as α-1-antitrypsin, α-actinin-1, carbonic anhydrase 4, high mobility group protein HMGI-C and hepatocellular carcinoma-derived growth factor. ISG15 ubiquitin like modifier (ISG15), a type I interferon-regulated ubiquitin-like molecule that plays crucial roles in modulating cell growth and cancer progression, has been reported to be highly expressed in various cancers (68–71). Similarly, ISG15 was well distinguished between patients with lymph node metastatic and non-metastatic PTMC. ISG15 knockdown reduced proliferation and infiltration of human PTC-derived BCPAP cells in vitro. Additionally, silencing ISG15 inhibited xenografted tumor growth in immunodeficient nude mice (72).
Targeted therapy remains a primary treatment for managing advanced TC. However, resistance to multiple drugs restricts the clinical utility of targeted agents. A study by Mishall et al (73) highlight the need for targeting multiple pathways simultaneously. They developed TC cells resistant to four Src inhibitors (dasatinib) and proteomic analysis revealed that the MAPK and AKT/mTOR pathways were critical for resistance to single-drug Src inhibitors.
Through proteogenomic analysis of matched normal thyroid tissue, primary tumor foci and LNM in one case, Krishnan et al (74) discovered a novel TFG-RET gene fusion. This fusion involves the 5′-terminal of Trk fusion gene (TFG) exons 1–4 and the 3′-terminal of RET tyrosine kinase. Further studies have revealed the involvement of HUWE1 in RET-induced oncogenic conversion of PTC and suggest that targeting HUWE1 or deubiquitinating enzymes (DUBs) is a promising therapeutic strategy for preventing RET-induced tumors. Clinical trials targeting the ubiquitinome are currently underway in other tumors and appear promising for PTC (75).
Proteomics has proven to be an efficient approach to identify potential biomarkers for DTC, but further research is needed to expand our understanding of DTC at the molecular level beyond the two aspects discussed earlier.
Discussion
DTC is the most prevalent type of TC worldwide and early detection remains challenging due to the lack of reliable and specific markers. Thus, identifying biomarkers that can aid in better diagnostic stratification, prognosis prediction and precise treatment is crucial. Recent research has focused on exploring the molecular mechanisms of DTC at the transcript and protein abundance levels. With the advancement of high-resolution histology technologies, even small changes in transcriptional, translational and post-translational modifications have been uncovered, which were previously undetectable using genomic or proteomic approaches. These research findings can serve as a foundation for future studies aimed at translating these results into clinical practice, meeting the demand for clinical stratification of DTC management in the era of precision medicine.
Acknowledgements
Not applicable.
Funding
Funding: The present study was supported by the NSFC program of Prof. GZ: 177Lu-CNP-iRGD Nanoprobes for Tracing and Targeting Cervical Lymph Node Metastasis of Thyroid Papillary Carcinoma (grant no. 81860313).
Availability of data and materials
Data sharing is not applicable to this article, as no data sets were generated or analyzed during the current study.
Authors' contributions
SY conceived, designed, wrote and revised the manuscript. RH, DF and JF were involved in the design and revision of the manuscript. GZ reviewed and revised the manuscript. All authors have read and approved the final manuscript. Data authentication is not applicable.
Ethics approval and consent to participate
Not applicable.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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