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β‑adrenergic receptor activation promotes the proliferation of HepG2 cells via the ERK1/2/CREB pathways

  • Authors:
    • Xingcheng Lin
    • Jingjing He
    • Fuhong Liu
    • Lehui Li
    • Longhua Sun
    • Liyan Niu
    • Haolin Xi
    • Yuan Zhan
    • Xiaohua Liu
    • Ping Hu

  • View Affiliations

  • Published online on: October 17, 2023     https://doi.org/10.3892/ol.2023.14106
  • Article Number: 519

  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Primary liver cancer is one of the most frequently diagnosed malignant tumors seen in clinics, and typically exhibits aggressive invasive behaviors, a poor prognosis, and is associated with high mortality rates. Long‑term stress exposure causes norepinephrine (NE) release and activates the β‑Adrenergic receptor (β‑AR), which in turn exacerbates the occurrence and development of different types of cancers; however, the molecular mechanisms of β‑AR in liver cancer are not fully understood. In the present study, reverse transcription (RT)‑PCR and RT‑quantitative PCR showed that β‑AR expression was upregulated in human liver cancer cells (HepG2) compared with normal liver cells (LO2). Moreover, NE treatment promoted the growth of HepG2 cells, which could be blocked by propranolol, a β‑AR antagonist. Notably, NE had no significant effect on the migration and epithelial‑mesenchymal transition in HepG2 cells. Further experiments revealed that NE increased the phosphorylation levels of the extracellular signal‑regulated kinase 1/2 (ERK1/2) and cyclic adenosine monophosphate response element‑binding protein (CREB), while inhibition of ERK1/2 and CREB activation significantly blocked NE‑induced cell proliferation. In summary, the findings of the present study suggested that β‑adrenergic receptor activation promoted the proliferation of HepG2 cells through ERK1/2/CREB signaling pathways.
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December-2023
Volume 26 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Lin X, He J, Liu F, Li L, Sun L, Niu L, Xi H, Zhan Y, Liu X, Hu P, Hu P, et al: β‑adrenergic receptor activation promotes the proliferation of HepG2 cells via the ERK1/2/CREB pathways. Oncol Lett 26: 519, 2023.
APA
Lin, X., He, J., Liu, F., Li, L., Sun, L., Niu, L. ... Hu, P. (2023). β‑adrenergic receptor activation promotes the proliferation of HepG2 cells via the ERK1/2/CREB pathways. Oncology Letters, 26, 519. https://doi.org/10.3892/ol.2023.14106
MLA
Lin, X., He, J., Liu, F., Li, L., Sun, L., Niu, L., Xi, H., Zhan, Y., Liu, X., Hu, P."β‑adrenergic receptor activation promotes the proliferation of HepG2 cells via the ERK1/2/CREB pathways". Oncology Letters 26.6 (2023): 519.
Chicago
Lin, X., He, J., Liu, F., Li, L., Sun, L., Niu, L., Xi, H., Zhan, Y., Liu, X., Hu, P."β‑adrenergic receptor activation promotes the proliferation of HepG2 cells via the ERK1/2/CREB pathways". Oncology Letters 26, no. 6 (2023): 519. https://doi.org/10.3892/ol.2023.14106