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Article Open Access

Association between microsatellite instability status, clinicopathological features and mitochondrial DNA amplification in patients with colorectal cancer

  • Authors:
    • Junmi Lu
    • Hong Tan
    • Tao Guo
    • Xi Chen
    • Zhongyi Tong
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Department of Emergency Medicine, The Second Xiangya Hospital, Emergency Medicine and Difficult Diseases Institute, Central South University, Changsha, Hunan 410011, P.R. China, Department of Pediatrics, Children's Nutrition Research Center, Baylor College of Medicine, Houston, TX 77030, USA
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 564
    |
    Published online on: September 26, 2024
       https://doi.org/10.3892/ol.2024.14698
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Abstract

The relationship between BRAF‑V600E mutations, mitochondrial DNA amplification and microsatellite instability‑high (MSI‑H) in colorectal cancer (CRC) has yet to be fully elucidated. The aim of the present study was to assess the association between the MSI status and BRAF‑V600E gene mutations/clinicopathological features/mitochondrial DNA amplification in CRC. A non‑interventional study analysis was performed using the clinicopathological features of 455 patients with CRC. Immunohistochemistry was used to evaluate four mismatch repair proteins (MutS homolog 2, MutS homolog 6, MutL homolog 1 and postmeiotic segregation increased 2), Ki‑67 index, and programmed cell death protein 1 (PD‑1) and programmed cell death‑ligand 1 (PD‑L1) expression. Additionally, PCR coupled with capillary electrophoresis were used to ascertain the MSI status. Moreover, amplification refractory mutation system‑PCR was used to detect BRAF‑V600E gene mutation and fluorescence in situ hybridization analysis was used to assess mitochondrial DNA. A total of 455 patients were divided into the MSI high (MSI‑H) group (n=52) and microsatellite stability (MSS) group (n=403) based on their MSI status. Compared with the results of immunohistochemistry of four mismatch repair proteins, the consistency rate between mismatch repair protein deficiency and MSI was 94.23%. There were significant differences in PD‑L1, primary tumor site, clinical stage, degree of differentiation, tumor size, lymph node metastasis and the occurrence of multiple primary tumors between the MSI‑H group and MSS group (P<0.05 or P<0.001). However, there were no significant differences for sex, age, PD‑1, Ki‑67 expression and BRAF‑V600E. The 24‑60‑month survival rate of the patients in the MSI‑H group was significantly higher than that of those in the MSS group (P<0.05). Furthermore, the number of mitochondrial DNA was significantly amplified in the MSI‑H group. In conclusion, the present study demonstrated thatthecombined detection of PD‑L1 and MSI in patients with CRC can provide more accurate and effective guidance for personalized treatment.
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View References

1 

Scanu AM and De Miglio MR: Therapeutic landscapes in colorectal carcinoma. Medicina. 59:8212023. View Article : Google Scholar : PubMed/NCBI

2 

Riihimaki M, Hemminki A, Sundquist J and Hemminki K: Patterns of metastasis in colon and rectal cancer. Sci Rep. 6:297652016. View Article : Google Scholar : PubMed/NCBI

3 

Li F, Lin Y, Li R, Shen X, Xiang M, Xiong G, Zhang Ke, Xia T, Guo J, Miao Z, et al: Molecular targeted therapy for metastatic colorectal cancer: Current and evolving approaches. Fron Pharmacol. 14:11656662023. View Article : Google Scholar : PubMed/NCBI

4 

Booker BD, Markt SC, Schumacher FR, Rose J, Cooper G, Eva Selfridge J and Koroukian SM: Variation in KRAS/NRAS/BRAF-mutation status by age, sex, and race/ethnicity among a large cohort of patients with metastatic colorectal cancer (mCRC). J Gastrointest Cancer. 55:237–246. 2024. View Article : Google Scholar : PubMed/NCBI

5 

Lin KX, Istl AC, Quan D, Skaro A, Tang E and Zheng X: PD-1 and PD-L1 inhibitors in cold colorectal cancer: Challenges and strategies. Cancer Immunol Immunother. 72:3875–3893. 2023. View Article : Google Scholar : PubMed/NCBI

6 

Afrăsânie VA, Marinca MV, Alexa-Stratulat T, Gafton B, Păduraru M, Adavidoaiei AM, Miron L and Rusu C: KRAS, NRAS, BRAF, HER2 and microsatellite instability in metastatic colorectal cancer-Practical implications for the clinician. Radiol Oncol. 53:265–274. 2019. View Article : Google Scholar : PubMed/NCBI

7 

Boland CR and Goel A: Microsatellite instability in colorectal cancer. Gastroenterology. 138:2073–2087. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Taieb J, Svrcek M, Cohen R, Basile D, Tougeron D and Phelip JM: Deficient mismatch repair/microsatellite unstable colorectal cancer: Diagnosis, prognosis and treatment. Eur J Cancer. 175:136–157. 2022. View Article : Google Scholar : PubMed/NCBI

9 

Liu Y, Sun Y, Guo Y, Shi X, Chen X, Feng W, Wu LL, Zhang J, Yu S, Wang Y and Shi Y: An overview: The diversified role of mitochondria in cancer metabolism. Int J Biol Sci. 19:897–915. 2023. View Article : Google Scholar : PubMed/NCBI

10 

Casanova A, Wevers A, Navarro-Ledesma S and Pruimboom L: Mitochondria: It is all about energy. Front Physiol. 14:11142312023. View Article : Google Scholar : PubMed/NCBI

11 

Tan H, Huang F, Huang M, Wu X and Tong Z: HSF1 attenuates the release of inflammatory cytokines induced by lipopolysaccharide through transcriptional regulation of ATG10. Microbiol Spectr. 11:e03059222023. View Article : Google Scholar : PubMed/NCBI

12 

Wang Y, Zhou J, Meng N, Yang B, Zhu C, Jiang B, Wang S and Chen X: The occurrence, progression and development of four types of gastric mucosal atrophic lesions and their histopathological characteristics. Gastric Cancer. 26:721–733. 2023. View Article : Google Scholar : PubMed/NCBI

13 

Fan A, Wang B, Wang X, Nie Y, Fan D, Zhao X and Lu Y: Immunotherapy in colorectal cancer: Current achievements and future perspective. Int J Biol Sci. 17:3837–3849. 2021. View Article : Google Scholar : PubMed/NCBI

14 

Mei WJ, Mi M, Qian J, Xiao N, Yuan Y and Ding PR: Clinicopathological characteristics of high microsatellite instability/mismatch repair-deficient colorectal cancer: A narrative review. Front Immunol. 13:10195822022. View Article : Google Scholar : PubMed/NCBI

15 

Lin A, Zhang J and Luo P: Crosstalk between the MSI status and tumor microenvironment in colorectal cancer. Frontiers Immuno. 11:20392020. View Article : Google Scholar : PubMed/NCBI

16 

Wang C, Kuang W, Zeng J, Ren Y, Liu Q, Sun H, Feng M and Liang D: A retrospective study of consistency between immunohistochemistry and polymerase chain reaction of microsatellite instability in endometrial cancer. Peer J. 11:e159202023. View Article : Google Scholar : PubMed/NCBI

17 

Chen J, Yan Q, Sun J, Wang Q, Tao Y, Xiao D and Xie B: Microsatellite status detection of colorectal cancer: Evaluation of inconsistency between PCR and IHC. J Cancer. 14:1132–1140. 2023. View Article : Google Scholar : PubMed/NCBI

18 

Jaffrelot M, Fares N, Brunac AC, Laurenty AP, Danjoux M, Grand D, Icher S, Meilleroux J, Mery E, Buscail E, et al: An unusual phenotype occurs in 15% of mismatch repair-deficient tumors and is associated with non-colorectal cancers and genetic syndromes. Mod Pathol. 35:427–437. 2022. View Article : Google Scholar : PubMed/NCBI

19 

Alikhani M, Saberi S, Esmaeili M, Michel V, Tashakoripour M, Abdirad A, Aghakhani A, Eybpoosh S, Vosough M, Mohagheghi MA, et al: Mitochondrial DNA copy number variations and serum pepsinogen levels for risk assessment in gastric cancer. Iran Biomed J. 25:323–333. 2021.PubMed/NCBI

20 

Copur MS and Manapuram S: Multiple primary tumors over a lifetime. Oncology. 33:6293842019.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lu J, Tan H, Guo T, Chen X and Tong Z: Association between microsatellite instability status, clinicopathological features and mitochondrial DNA amplification in patients with colorectal cancer. Oncol Lett 28: 564, 2024.
APA
Lu, J., Tan, H., Guo, T., Chen, X., & Tong, Z. (2024). Association between microsatellite instability status, clinicopathological features and mitochondrial DNA amplification in patients with colorectal cancer. Oncology Letters, 28, 564. https://doi.org/10.3892/ol.2024.14698
MLA
Lu, J., Tan, H., Guo, T., Chen, X., Tong, Z."Association between microsatellite instability status, clinicopathological features and mitochondrial DNA amplification in patients with colorectal cancer". Oncology Letters 28.6 (2024): 564.
Chicago
Lu, J., Tan, H., Guo, T., Chen, X., Tong, Z."Association between microsatellite instability status, clinicopathological features and mitochondrial DNA amplification in patients with colorectal cancer". Oncology Letters 28, no. 6 (2024): 564. https://doi.org/10.3892/ol.2024.14698
Copy and paste a formatted citation
x
Spandidos Publications style
Lu J, Tan H, Guo T, Chen X and Tong Z: Association between microsatellite instability status, clinicopathological features and mitochondrial DNA amplification in patients with colorectal cancer. Oncol Lett 28: 564, 2024.
APA
Lu, J., Tan, H., Guo, T., Chen, X., & Tong, Z. (2024). Association between microsatellite instability status, clinicopathological features and mitochondrial DNA amplification in patients with colorectal cancer. Oncology Letters, 28, 564. https://doi.org/10.3892/ol.2024.14698
MLA
Lu, J., Tan, H., Guo, T., Chen, X., Tong, Z."Association between microsatellite instability status, clinicopathological features and mitochondrial DNA amplification in patients with colorectal cancer". Oncology Letters 28.6 (2024): 564.
Chicago
Lu, J., Tan, H., Guo, T., Chen, X., Tong, Z."Association between microsatellite instability status, clinicopathological features and mitochondrial DNA amplification in patients with colorectal cancer". Oncology Letters 28, no. 6 (2024): 564. https://doi.org/10.3892/ol.2024.14698
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