Open Access

MicroRNA‑24 alleviates colorectal cancer progression via a rs28382740 single nucleotide polymorphism in the long noncoding region of X‑linked inhibitor of apoptosis protein

  • Authors:
    • Yu-Feng Tian
    • Chi-Jung Huang
    • Chih-Yi Liu
    • Shung-Haur Yang
    • Chih-Sheng Hung
    • Kai-Yuan Lin
    • Ching-Long Lai
    • Chun-Chao Chang
  • View Affiliations

  • Published online on: October 4, 2024     https://doi.org/10.3892/ol.2024.14724
  • Article Number: 591
  • Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Colorectal cancer (CRC) is one of the most prevalent malignant diseases worldwide. Recurrence is associated with the poor survival of patients with CRC. Targeted therapy and precision medicine for recurrent CRC may improve the clinical outcome. Therefore, finding biomarkers that can detect CRC early, assess its prognosis and survival, and predict its treatment response is key to improving the clinical prognosis. The aim of this study was to assess CRC recurrence by analyzing molecular differences using postoperative specimens. Whole‑exome sequencing was first used to evaluate the molecular differences in CRC tissues from patients with recurrent disease, and the results were then verified with tissue array methods. The regulation of single nucleotide polymorphisms (SNPs) in long noncoding regions of interest was analyzed in the presence of target microRNAs (miRs) using luciferase assays. The results demonstrated that in patients with recurrent CRC, the G allele was mainly detected at the rs28382740 SNP in the 3'‑untranslated region of the X‑linked inhibitor of apoptosis (XIAP)‑encoding gene. From the tissue arrays, 60% (3/5) of patients with the G allele of the rs28382740 SNP were diagnosed with CRC recurrence, whilst only 10% (1/10) of patients without the G allele had recurrent CRC (P=0.077). Furthermore, XIAP levels were high in non‑CRC (50%; 2/4) and CRC (75%; 3/4) tissues of patients with recurrent disease and CRC (54.5%; 6/11) tissues of patients without recurrent disease. However, but only 9.1% (1/11) of non‑CRC tissues of nonrecurrent patients had significantly high XIAP expression levels (P=0.022). Using a luciferase assay, it was demonstrated that miR‑24s (miR‑24‑1‑5p and miR‑24‑2‑5p) targeting the rs28382740 SNP reduced XIAP levels in CRC cells with rs28382740 SNP genotype G. These results indicate that apoptosis‑related proteins, such as XIAP, may be therapeutic targets or biomarkers for tumor development. The data from the present study support an inhibitory effect of miR‑24s on XIAP expression. However, this inhibitory potency depends on the rs28382740 SNP genotype and may alleviate CRC progression by regulating the expression of XIAP.
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December-2024
Volume 28 Issue 6

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Spandidos Publications style
Tian Y, Huang C, Liu C, Yang S, Hung C, Lin K, Lai C and Chang C: MicroRNA‑24 alleviates colorectal cancer progression via a rs28382740 single nucleotide polymorphism in the long noncoding region of X‑linked inhibitor of apoptosis protein. Oncol Lett 28: 591, 2024.
APA
Tian, Y., Huang, C., Liu, C., Yang, S., Hung, C., Lin, K. ... Chang, C. (2024). MicroRNA‑24 alleviates colorectal cancer progression via a rs28382740 single nucleotide polymorphism in the long noncoding region of X‑linked inhibitor of apoptosis protein. Oncology Letters, 28, 591. https://doi.org/10.3892/ol.2024.14724
MLA
Tian, Y., Huang, C., Liu, C., Yang, S., Hung, C., Lin, K., Lai, C., Chang, C."MicroRNA‑24 alleviates colorectal cancer progression via a rs28382740 single nucleotide polymorphism in the long noncoding region of X‑linked inhibitor of apoptosis protein". Oncology Letters 28.6 (2024): 591.
Chicago
Tian, Y., Huang, C., Liu, C., Yang, S., Hung, C., Lin, K., Lai, C., Chang, C."MicroRNA‑24 alleviates colorectal cancer progression via a rs28382740 single nucleotide polymorphism in the long noncoding region of X‑linked inhibitor of apoptosis protein". Oncology Letters 28, no. 6 (2024): 591. https://doi.org/10.3892/ol.2024.14724