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Anticancer effects of the Kiperin Purple Collagen complex: Evidence from HCT116 and MIA PaCa‑2 cell models

  • Authors:
    • Lutfiye Karcioglu Batur
    • Cuneyd Yavas
    • Nezih Hekim
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Biruni University, Istanbul 34015, Turkey
    Copyright: © Batur et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 560
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    Published online on: October 1, 2025
       https://doi.org/10.3892/ol.2025.15306
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Abstract

Purple Collagen is a bioactive complex containing double‑hydrolyzed collagens (types I, II, III, V and X) and various bioactive components (inulin, elderberry extract, magnesium citrate and malate, eggshell membrane, bromelain, black cumin extract and liposomal vitamin C). These bioactive compounds have attracted increasing scientific interest due to their ability to modulate key cancer‑associated pathways, including the inhibition of cell proliferation and migration, suppression of oxidative stress via free radical scavenging and induction of apoptosis through mitochondrial and caspase‑dependent mechanisms. While individual components of the Purple Collagen complex (PCC) have been associated with various health benefits, their combined effects on cancer cell behavior remain largely unexplored. The present study investigated the effects of PCC on cell proliferation, migration, oxidative stress and apoptosis in two cancer cell lines: HCT116 (colorectal carcinoma) and MIA PaCa‑2 (pancreas carcinoma). The cell viability, migration, oxidative stress [total oxidant status (TOS)/total antioxidant status (TAS)] and apoptotic and cell cycle regulatory markers (BAX, BCL2, TP53 and cyclin‑dependent kinase inhibitor 1) were analyzed following treatment with 1 µg/ml PCC. Experiments were performed in vitro, and statistical significance was assessed. Cell counts for viability and proliferation analyses were obtained with a Thoma hemocytometer after trypan blue staining. PCC treatment significantly reduced cell proliferation in HCT116 and MIA PaCa‑2 cells (P=0.0141 and P=0.0004, respectively). Migration assays demonstrated significant reductions at intermediate time points in both cell lines, HCT116 (P=0.0091) and MIA PaCa‑2 cells (P=0.01). TOS and TAS levels revealed a cell‑type‑specific response, with a marked TAS decrease in HCT116 (P=0.0095). PCC caused an apoptotic and cell cycle regulatory effect, affecting BCL2 and p21 expression levels significantly in both cell lines (P<0.05). PCC exhibits notable cell‑type‑specific effects, inhibiting proliferation and migration in colon and pancreas carcinoma cells while modulating oxidative stress and apoptosis. The findings highlight the potential of bioactive compounds as selective modulators of cancer cell behavior. Further in vivo studies are required to evaluate these effects and their clinical relevance. 
View Figures

Figure 1

Effect of 1 µg/ml Kiperin Purple
Collagen Complex treatment on cell proliferation in HCT116 and MIA
PaCa-2 cell lines. Bar graphs represent mean ± standard deviation
of three independent experiments. *P<0.05, ***P<0.01 vs.
control group. Treatment, 1 µg/ml Kiperin Purple Collagen complex;
Control, untreated.

Figure 2

Representative images of the wound
healing assay of untreated or Kiperin Purple Collagen
complex-treated MIA PaCa-2 cells.

Figure 3

Representative images of the wound
assay of untreated or Kiperin Purple Collagen complex-treated
HCT116 cells.

Figure 4

Expression levels of BAX, BCL2,
TP53 and p21 in HCT116 and MIA PaCa-2 cell groups
treated with 1 µg/ml Purple Collagen Complex from three independent
experiments. Data presented as mean ± standard deviation of three
independent experiments. **P<0.01, ***P<0.001 vs. control
group. ns, non-significant; Treatment, 1 µg/ml Kiperin Purple
Collagen complex; Control, untreated.
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Copy and paste a formatted citation
Spandidos Publications style
Batur LK, Yavas C and Hekim N: Anticancer effects of the Kiperin Purple Collagen complex: Evidence from HCT116 and MIA PaCa‑2 cell models. Oncol Lett 30: 560, 2025.
APA
Batur, L.K., Yavas, C., & Hekim, N. (2025). Anticancer effects of the Kiperin Purple Collagen complex: Evidence from HCT116 and MIA PaCa‑2 cell models. Oncology Letters, 30, 560. https://doi.org/10.3892/ol.2025.15306
MLA
Batur, L. K., Yavas, C., Hekim, N."Anticancer effects of the Kiperin Purple Collagen complex: Evidence from HCT116 and MIA PaCa‑2 cell models". Oncology Letters 30.6 (2025): 560.
Chicago
Batur, L. K., Yavas, C., Hekim, N."Anticancer effects of the Kiperin Purple Collagen complex: Evidence from HCT116 and MIA PaCa‑2 cell models". Oncology Letters 30, no. 6 (2025): 560. https://doi.org/10.3892/ol.2025.15306
Copy and paste a formatted citation
x
Spandidos Publications style
Batur LK, Yavas C and Hekim N: Anticancer effects of the Kiperin Purple Collagen complex: Evidence from HCT116 and MIA PaCa‑2 cell models. Oncol Lett 30: 560, 2025.
APA
Batur, L.K., Yavas, C., & Hekim, N. (2025). Anticancer effects of the Kiperin Purple Collagen complex: Evidence from HCT116 and MIA PaCa‑2 cell models. Oncology Letters, 30, 560. https://doi.org/10.3892/ol.2025.15306
MLA
Batur, L. K., Yavas, C., Hekim, N."Anticancer effects of the Kiperin Purple Collagen complex: Evidence from HCT116 and MIA PaCa‑2 cell models". Oncology Letters 30.6 (2025): 560.
Chicago
Batur, L. K., Yavas, C., Hekim, N."Anticancer effects of the Kiperin Purple Collagen complex: Evidence from HCT116 and MIA PaCa‑2 cell models". Oncology Letters 30, no. 6 (2025): 560. https://doi.org/10.3892/ol.2025.15306
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