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Article Open Access

SENP5 acts as a downstream target of androgen receptor and contributes to prostate cancer growth

  • Authors:
    • Ximin Chen
    • Xubo Gong
    • Jingcheng Zhang
    • Weiwei Liu
  • View Affiliations / Copyright

    Affiliations: Department of Medical Genetics and Prenatal Diagnosis, Sichuan Provincial Women's and Children's Hospital, The Affiliated Women's and Children's Hospital of Chengdu Medical College, Chengdu, Sichuan 610041, P.R. China, Department of Laboratory Medicine, Zhejiang University School of Medicine Second Affiliated Hospital, Hangzhou, Zhejiang 310009, P.R. China, Department of Hematology, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, Zhejiang 321000, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 51
    |
    Published online on: November 26, 2025
       https://doi.org/10.3892/ol.2025.15404
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Abstract

The androgen receptor (AR) signaling pathway plays an important role in prostate cancer (PCa) progression. In the present study, a significant co‑expression was found between SENPs and AR. In addition, SENP5 was obviously negatively correlated with overall survival and disease‑free survival in patients with PCa. Moreover, SENP5 silencing markedly inhibited the proliferation of PCa cells. Chromatin immunoprecipitation quantitative PCR assays further confirmed that AR could transcriptionally activate SENP5 expression. In summary, the present results suggested that SENP5 acts as a downstream target of AR and contributes to PCa growth, the underlying molecular mechanism of which needs further investigation.
View Figures

Figure 1

mRNA expression level of SENPs in
prostate cancer and adjacent normal tissues varies only slightly,
but there is significant co-expression with AR. (A) Gene expression
analysis of SENPs in normal and tumoral prostate tissues according
to the PRAD TCGA dataset. Results were obtained from the GEPIA
website. (B) Pearson's correlation analysis of SENPs with AR using
the PRAD TCGA dataset. Results were obtained from the GEPIA website
http://gepia2.cancer-pku.cn/. AR,
androgen receptor; PRAD, prostate adenocarcinoma; TCGA, The Cancer
Genome Atlas.

Figure 2

SENP5 acts as a downstream target of
AR and contributes to PCa growth. (A and B) Survival analysis of
patients with PCa stratified according high and low SENP5
expression using the prostate adenocarcinoma dataset from The
Cancer Genome Atlas. (A) Overall survival; (B) Disease-free
survival. (C and D) Immunofluorescence analysis of the expression
of SENP5 in PCa tissues. (C) Representative images (including low,
intermediate and high expression) from the immunofluorescence
analysis for SENP5 in primary PCa tissues and corresponding
adjacent normal prostate tissues. (D) Quantification of the
staining observed in 30 PCa cases. (E) LNCaP and LNCaP-AI cells
were transfected with siScr or siSENP5 for 48 h; western blots were
probed for levels of SENP5 and GAPDH was used as a loading control.
(F) Cell viability was determined using a Cell Counting Kit-8
assay. (G) LNCaP and LNCaP-AI cells were treated with MDV3100 (25
µM), DHT (10 nM) or vehicle alone for 48 h. The mRNA expression
level of SENP5 was measured using quantitative PCR. (H) LNCaP cells
were transfected with siAR or treated with MDV3100 (25 µM) for 48
h; then total protein cell lysates were prepared and immunoblot
analysis was performed using anti-SENP5 and anti- GAPDH antibodies.
(I) Chromatin immunoprecipitation-quantitative PCR showing the
occupancy of AR on the human SENP5 ARE region in LNCaP cells with
indicated treatment. All results are representative of three
experiments and expressed as the mean ± S.D. *P<0.05. AR,
androgen receptor; PCa, prostate cancer; si-, small interfering;
DHT, dihydrotestosterone.
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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Gong X, Zhang J and Liu W: SENP5 acts as a downstream target of androgen receptor and contributes to prostate cancer growth. Oncol Lett 31: 51, 2026.
APA
Chen, X., Gong, X., Zhang, J., & Liu, W. (2026). SENP5 acts as a downstream target of androgen receptor and contributes to prostate cancer growth. Oncology Letters, 31, 51. https://doi.org/10.3892/ol.2025.15404
MLA
Chen, X., Gong, X., Zhang, J., Liu, W."SENP5 acts as a downstream target of androgen receptor and contributes to prostate cancer growth". Oncology Letters 31.2 (2026): 51.
Chicago
Chen, X., Gong, X., Zhang, J., Liu, W."SENP5 acts as a downstream target of androgen receptor and contributes to prostate cancer growth". Oncology Letters 31, no. 2 (2026): 51. https://doi.org/10.3892/ol.2025.15404
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Gong X, Zhang J and Liu W: SENP5 acts as a downstream target of androgen receptor and contributes to prostate cancer growth. Oncol Lett 31: 51, 2026.
APA
Chen, X., Gong, X., Zhang, J., & Liu, W. (2026). SENP5 acts as a downstream target of androgen receptor and contributes to prostate cancer growth. Oncology Letters, 31, 51. https://doi.org/10.3892/ol.2025.15404
MLA
Chen, X., Gong, X., Zhang, J., Liu, W."SENP5 acts as a downstream target of androgen receptor and contributes to prostate cancer growth". Oncology Letters 31.2 (2026): 51.
Chicago
Chen, X., Gong, X., Zhang, J., Liu, W."SENP5 acts as a downstream target of androgen receptor and contributes to prostate cancer growth". Oncology Letters 31, no. 2 (2026): 51. https://doi.org/10.3892/ol.2025.15404
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