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Article

Predictive value analysis of CEA, CA125 and CYFRA21‑1 levels in evaluating the efficacy of tislelizumab combined with gemcitabine‑cisplatin chemotherapy in patients with advanced non‑small cell lung cancer

  • Authors:
    • Langyue Wang
    • Tianyu Gou
    • Yang Cui
    • Meng Li
    • Yu Wang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Medical Records, Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Medical Affairs, Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Medical Examination Center, Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
  • Article Number: 89
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    Published online on: December 30, 2025
       https://doi.org/10.3892/ol.2025.15442
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Abstract

While chemoimmunotherapy is the standard for advanced non‑small cell lung cancer (NSCLC), effective and accessible tools for monitoring early treatment response are needed. The present study evaluated the clinical efficacy and safety of tislelizumab + gemcitabine‑cisplatin (GP) chemotherapy vs. GP alone in NSCLC, and assessed serum CEA, CA125 and cytokeratin 19 fragment (CYFRA21‑1) levels for treatment monitoring. In total, 90 patients with advanced NSCLC (from January 2021 to December 2024) were randomized in a 1:1 manner to receive tislelizumab + GP (n=45) or GP chemotherapy alone (n=45). Outcomes included short‑term response [objective response rate (ORR) and disease control rate (DCR)], long‑term progression‑free survival (PFS) and overall survival (OS) (data cut‑off, May 31, 2025; median follow‑up, 23.5 months), 3‑month biomarker changes and adverse events (AEs). The tislelizumab + GP group demonstrated numerically higher ORR/DCR (P>0.05), significantly longer median PFS (20.8 vs. 10.0 months, P=0.03) and a trend toward improved OS (not reached vs. 16.0 months; P=0.095). Post‑treatment biomarker levels were significantly decreased in the study group (P<0.05) with comparable AEs. Receiver operating characteristic curves analyzed the diagnostic value of the combined biomarker panel compared with each biomarker individually for the prediction of short‑term efficacy, which demonstrated significantly enhanced performance for the combination (P<0.05). In conclusion, combined CEA, CA125 and CYFRA21‑1 effectively evaluated short‑term efficacy. Furthermore, tislelizumab + GP demonstrated favorable safety and improved survival outcomes (particularly PFS) within follow‑up.
View Figures

Figure 1

Receiver operating characteristic
curve analysis of serum biomarkers for the prediction of short-term
therapeutic efficacy of tislelizumab + gemcitabine-cisplatin
chemotherapy in patients with advanced non-small cell lung cancer.
CYFRA 21-1, cytokeratin 19 fragment.

Figure 2

Kaplan-Meier curve for PFS. The
present analysis compared PFS between the study group (tislelizumab
+ GP chemotherapy; n=45) and the control group (GP chemotherapy
alone; n=45). The study group exhibited a statistically significant
improvement in median PFS (20.8 vs. 10.0 months; hazard ratio,
0.66; 95% CI, 0.43–0.98; log-rank P=0.038). PFS, progression-free
survival; GP, gemcitabine-cisplatin.

Figure 3

Kaplan-Meier curve for OS. The
analysis compared OS between the study group (tislelizumab + GP
chemotherapy; n=45) and the control group (GP chemotherapy alone;
n=45). The study group exhibited a trend towards improved median
OS, although the difference was not statistically significant (not
reached vs. 16.0 months; hazard ratio, 0.71; 95% CI, 0.47–1.07;
log-rank P=0.095). OS, overall survival; GP,
gemcitabine-cisplatin.
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Copy and paste a formatted citation
Spandidos Publications style
Wang L, Gou T, Cui Y, Li M and Wang Y: Predictive value analysis of CEA, CA125 and CYFRA21‑1 levels in evaluating the efficacy of tislelizumab combined with gemcitabine‑cisplatin chemotherapy in patients with advanced non‑small cell lung cancer. Oncol Lett 31: 89, 2026.
APA
Wang, L., Gou, T., Cui, Y., Li, M., & Wang, Y. (2026). Predictive value analysis of CEA, CA125 and CYFRA21‑1 levels in evaluating the efficacy of tislelizumab combined with gemcitabine‑cisplatin chemotherapy in patients with advanced non‑small cell lung cancer. Oncology Letters, 31, 89. https://doi.org/10.3892/ol.2025.15442
MLA
Wang, L., Gou, T., Cui, Y., Li, M., Wang, Y."Predictive value analysis of CEA, CA125 and CYFRA21‑1 levels in evaluating the efficacy of tislelizumab combined with gemcitabine‑cisplatin chemotherapy in patients with advanced non‑small cell lung cancer". Oncology Letters 31.2 (2026): 89.
Chicago
Wang, L., Gou, T., Cui, Y., Li, M., Wang, Y."Predictive value analysis of CEA, CA125 and CYFRA21‑1 levels in evaluating the efficacy of tislelizumab combined with gemcitabine‑cisplatin chemotherapy in patients with advanced non‑small cell lung cancer". Oncology Letters 31, no. 2 (2026): 89. https://doi.org/10.3892/ol.2025.15442
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Gou T, Cui Y, Li M and Wang Y: Predictive value analysis of CEA, CA125 and CYFRA21‑1 levels in evaluating the efficacy of tislelizumab combined with gemcitabine‑cisplatin chemotherapy in patients with advanced non‑small cell lung cancer. Oncol Lett 31: 89, 2026.
APA
Wang, L., Gou, T., Cui, Y., Li, M., & Wang, Y. (2026). Predictive value analysis of CEA, CA125 and CYFRA21‑1 levels in evaluating the efficacy of tislelizumab combined with gemcitabine‑cisplatin chemotherapy in patients with advanced non‑small cell lung cancer. Oncology Letters, 31, 89. https://doi.org/10.3892/ol.2025.15442
MLA
Wang, L., Gou, T., Cui, Y., Li, M., Wang, Y."Predictive value analysis of CEA, CA125 and CYFRA21‑1 levels in evaluating the efficacy of tislelizumab combined with gemcitabine‑cisplatin chemotherapy in patients with advanced non‑small cell lung cancer". Oncology Letters 31.2 (2026): 89.
Chicago
Wang, L., Gou, T., Cui, Y., Li, M., Wang, Y."Predictive value analysis of CEA, CA125 and CYFRA21‑1 levels in evaluating the efficacy of tislelizumab combined with gemcitabine‑cisplatin chemotherapy in patients with advanced non‑small cell lung cancer". Oncology Letters 31, no. 2 (2026): 89. https://doi.org/10.3892/ol.2025.15442
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