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Circular RNA hsa_circ_0006168: A potential biomarker for the diagnosis and prognosis of prostate cancer

  • Authors:
    • Ye Ding
    • Mao-Tong Liu
    • Na Yu
    • Liusijie Gao
    • Li-Ping Chen
  • View Affiliations / Copyright

    Affiliations: Department of Medical Laboratory, Affiliated Hospital 2 of Nantong University, Nantong First People's Hospital, Nantong, Jiangsu 226000, P.R. China, Department of Radiology, Affiliated Nantong Hospital 3 of Nantong University, Nantong Third People's Hospital, Nantong, Jiangsu 226000, P.R. China, Center for Reproductive Medicine, Affiliated Hospital 2 of Nantong University, Nantong First People's Hospital, Nantong, Jiangsu 226000, P.R. China
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 102
    |
    Published online on: January 9, 2026
       https://doi.org/10.3892/ol.2026.15455
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Abstract

The limited specificity of prostate‑specific antigen for prostate cancer (PCa) necessitates novel, non‑invasive biomarkers. The present study aimed to investigate the expression of the circular (circ)RNA hsa_circ_0006168 in PCa and its association with clinical parameters, and to evaluate its potential as a diagnostic and prognostic biomarker for PCa. Serum samples were collected from patients with PCa, benign prostatic hyperplasia (BPH) and healthy controls. Reverse transcription‑quantitative PCR was used to measure the expression level of hsa_circ_0006168 in the sera. The association between the expression of hsa_circ_0006168 and several clinicopathological parameters was subsequently analyzed, and univariate and multivariate Cox regression analyses were performed to identify factors affecting prognosis. The expression level of hsa_circ_0006168 in the sera of patients with PCa was revealed to be significantly higher compared with that in the patients with BPH and the healthy controls. The area under the receiver operating characteristic curve was 0.773. The clinicopathological parameter analysis revealed that a high expression level of hsa_circ_0006168 was positively associated with the Gleason score, consistent with circRNA sequencing data. Cox regression analysis revealed that tumor‑node‑metastasis staging was an independent risk factor affecting prognosis. Furthermore, the expression level of hsa_circ_0006168, the Gleason score and bone metastasis were identified as significant risk factors according to the univariate Cox regression analysis; however, no significant factors were identified in the multivariate Cox regression analysis. In conclusion, serum hsa_circ_0006168 may be a promising biomarker for the early diagnosis and prognosis evaluation of PCa.

View Figures

Figure 1

Identification of differentially
expressed circRNAs in prostate cancer. Heatmaps of (A) GSE15792 and
(B) GSE113153 datasets. Volcano plots of the (C) GSE15792 and (D)
GSE113153 datasets. (E) Venn diagram based on the intersection of
the differentially expressed genes. FC, fold change.

Figure 2

Expression levels and diagnostic value
of serum hsa_circ_0006168 in patients with PCa. (A) Expression
levels of hsa_circ_0006168 in the sera of patients with PCa or BPH,
and healthy controls. (B) hsa_circ_0006168 was shown to have good
diagnostic value as a PCa biomarker. ***P<0.001;
****P<0.0001. PCa, prostate cancer; BPH, benign prostatic
hyperplasia; NS, not significant; ROC, Receiver Operating
Characteristic.

Figure 3

Expression of hsa_circ_0006168 in the
cell lines utilized in the present study. *P<0.05;
***P<0.001vs. RWPE-1.

Figure 4

Methodological evaluation of
hsa_circ_0006168. The linearity of (A) hsa_circ_0006168 and (B)
GAPDH were found to be acceptable. The stability of
hsa_circ_0006168 (C) after repeated freezing and thawing, and (D)
at room temperature.

Figure 5

Construction and evaluation of a
nomogram for predicting overall survival in patients with prostate
cancer. (A) Nomogram for predicting survival probability. (B)
Calibration curve for survival prediction at 20 months. (C)
Decision curve analysis, highlighting clinical utility. (D)
Kaplan-Meier survival curves for patients with prostate cancer,
stratified according to the expression levels of hsa_circ_0006168.
TNM, tumor-node-metastasis; HR, hazard ratio; CI, confidence
interval.

Figure 6

Bioinformatics analysis of the
function, structure and targets of hsa_circ_0006168. (A) Gene
Ontology and (B) Kyoto Encyclopedia of Genes and Genomes pathway
analysis of hsa_circ_0006168. (C) Sequence and structural analysis
of hsa_circ_0006168. (D) Differential microRNA analysis in prostate
cancer tissue and cells.
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Copy and paste a formatted citation
Spandidos Publications style
Ding Y, Liu M, Yu N, Gao L and Chen L: <p>Circular RNA hsa_circ_0006168: A potential biomarker for the diagnosis and prognosis of prostate cancer</p>. Oncol Lett 31: 102, 2026.
APA
Ding, Y., Liu, M., Yu, N., Gao, L., & Chen, L. (2026). <p>Circular RNA hsa_circ_0006168: A potential biomarker for the diagnosis and prognosis of prostate cancer</p>. Oncology Letters, 31, 102. https://doi.org/10.3892/ol.2026.15455
MLA
Ding, Y., Liu, M., Yu, N., Gao, L., Chen, L."<p>Circular RNA hsa_circ_0006168: A potential biomarker for the diagnosis and prognosis of prostate cancer</p>". Oncology Letters 31.3 (2026): 102.
Chicago
Ding, Y., Liu, M., Yu, N., Gao, L., Chen, L."<p>Circular RNA hsa_circ_0006168: A potential biomarker for the diagnosis and prognosis of prostate cancer</p>". Oncology Letters 31, no. 3 (2026): 102. https://doi.org/10.3892/ol.2026.15455
Copy and paste a formatted citation
x
Spandidos Publications style
Ding Y, Liu M, Yu N, Gao L and Chen L: <p>Circular RNA hsa_circ_0006168: A potential biomarker for the diagnosis and prognosis of prostate cancer</p>. Oncol Lett 31: 102, 2026.
APA
Ding, Y., Liu, M., Yu, N., Gao, L., & Chen, L. (2026). <p>Circular RNA hsa_circ_0006168: A potential biomarker for the diagnosis and prognosis of prostate cancer</p>. Oncology Letters, 31, 102. https://doi.org/10.3892/ol.2026.15455
MLA
Ding, Y., Liu, M., Yu, N., Gao, L., Chen, L."<p>Circular RNA hsa_circ_0006168: A potential biomarker for the diagnosis and prognosis of prostate cancer</p>". Oncology Letters 31.3 (2026): 102.
Chicago
Ding, Y., Liu, M., Yu, N., Gao, L., Chen, L."<p>Circular RNA hsa_circ_0006168: A potential biomarker for the diagnosis and prognosis of prostate cancer</p>". Oncology Letters 31, no. 3 (2026): 102. https://doi.org/10.3892/ol.2026.15455
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